List of Publications

Publications in Refereed Journals

  1. Makovitzki, A., Baram, Y., and Shai, Y. (2008) " Palmitoyl Di- and Tri-Cationic Antimicrobial Lipopeptides: In Vitro and In Vivo Activities, Self-Assembly to Nano Structures and a Plausible Mode of Action". Biochemistry, in press.

  2. Mangoni, M., Epand, R., Rosenfeld, Y., Pleg, A., Barra, D., Epand, R., and Shai, Y. (2008) "Lipopolysaccharide, a Key Molecule Involved in the Synergism Between Temporins in Inhibiting Bacterial Growth and in Endotoxin Neutralization". J. Biol. Chem., 283, 22907-22917.

  3. Vallon-Eberhard, A., Makovitzki, A., Beauvais, A., Latgé, J., Jung, S., and Shai, Y. (2008) "Efficient Clearance of Aspergillus Fumigatus by Ultrashort Anti-Microbial Lipopeptides in Murine Lungs". Antimicrob. Agents Chemother., 52, 3118-3126.

  4. Rosenfeld, Y., Sahl, H. G., and Shai, Y. (2008) "Parameters involved in antimicrobial and endotoxin detoxification activities of antimicrobial peptides". Biochemistry, 47, 6468-6478.

  5. Cohen, T., Pevsner-Fischer, M., Cohen, N., Cohen, I. R., and Shai, Y. (2008) "Characterization of the interacting domain of the HIV-1 fusion peptide with the transmembrane domain of the T-cell receptor". Biochemistry, 47, 4826-4833.

  6. Pag, U., Oedenkoven, M., Sass, V., Shai, Y., Shamova, O., Antcheva, N., Tossi, A., and Sahl, H. G. (2008) "Analysis of in vitro activities and modes of action of synthetic antimicrobial peptides derived from an {alpha}-helical 'sequence template'". J. Antimicrob. Chemother., 61, 341-352.

  7. Lev, N., and Shai, Y. (2007) "Fatty Acids can Substitute the HIV Fusion Peptide in Lipid Merging and Fusion: An Analogy between Viral and Palmitoylated Eukaryotic Fusion Proteins". J. Mol. Biol., 374, 220-230.

  8. Bloch, I., Quintana, F. J., Gerber, D., Cohen, T., Cohen, I. R., and Shai, Y. (2007) "T-cell inactivation and immunosuppressive activity induced by HIV gp41 via novel interacting motif". FASEB J., 21, 393-401.

  9. Huberman, L., Gollop, N., Mumcuoglu, K. Y., Breuer, E., Bhusare, S. R., Shai, Y., and Galun, R. (2007) Antibacterial substances of low molecular weight isolated from the blowfly, Lucilia sericata". Med. Vet. Entomol., 21, 127-131.

  10. Kristian, S. A., Timmer, A. M., Liu, G. Y., Lauth, X., Sal-Man, N., Rosenfeld, Y., Shai, Y., Gallo, R. L., and Nizet, V. (2007) "Impairment of innate immune killing mechanisms by bacteriostatic antibiotics". FABEB J., 21, 1107-1116.

  11. Lorin, A., Charloteaux, B., Fridmann-Sirkis, Y., Thomas, A., Shai, Y., and Brasseur, R. (2007) "Mode of membrane interaction and fusogenic properties of a de novo transmembrane model peptide depend on the length of the hydrophobic core". J. Biol. Chem., 282, 18388-18396.

  12. Makovitzki, A., Viterbo, A., Brotman, Y., Chet, I., and Shai, Y. (2007) "Inhibition of Fungal and Bacterial Plant Pathogens in Vitro and in Planta with Ultrashort Cationic Lipopeptides". Appl Environ Microbiol., in press.

  13. Quintana, F. J., Gerber, D., Bloch, I., Cohen, I. R., and Shai, Y. (2007) "A structurally altered D,L-amino acid TCRalpha transmembrane peptide interacts with the TCRalpha and inhibits T-cell activation in vitro and in an animal model". Biochemistry, 46, 2317-2325.

  14. Sal-Man, N., Gerber, D., Bloch, I., and Shai, Y. (2007) "Specificity in transmembrane helix-helix interactions mediated by aromatic residues". J. Biol. Chem., 282, 19753-19761.

  15. Wexler-Cohen, Y., and Shai, Y. (2007) "Demonstrating the C Terminal Boundary of the HIV 1 Fusion Conformation in a Dynamic Ongoing Fusion Process and Implication for Fusion Inhibition". FASEB J., in press.

  16. Wexler-Cohen, Y., Johnson, B. T., Puri, A., Blumenthal, R., and Shai, Y. (2006) "Structurally altered peptides reveal an important role for N-terminal heptad repeat binding and stability in the inhibitory action of HIV-1 peptide DP178". J. Biol. Chem., 281, 9005-9010.

  17. Tomasinsig, L., Skerlavaj, B., Papo, N., Giabbai, B., Shai, Y., and Zanetti, M. (2006) "Mechanistic and Functional Studies of the Interaction of a Proline-rich Antimicrobial Peptide with Mammalian Cells". J. Biol. Chem., 281, 383-391.

  18. Sackett, K., Wexler-Cohen, Y., and Shai, Y. (2006) "Characterization of the HIV N-terminal fusion peptide-containing region in context of key gp41 fusion conformations". J. Biol. Chem., 281, 21755-21762.

  19. Rosenfeld, Y., and Shai, Y. (2006) "Lipopolysaccharide (Endotoxin)-host defense antibacterial peptides interactions: role in bacterial resistance and prevention of sepsis". Biochim. Biophys. Acta., 1758, 1513-1522.

  20. Rosenfeld, Y., Barra, D., Simmaco, M., Shai, Y., and Mangoni, M. L. (2006) "A Synergism between Temporins toward Gram-negative Bacteria Overcomes Resistance Imposed by the Lipopolysaccharide Protective Layer". J. Biol. Chem., 281, 28565-28574.

  21. Rosenfeld, Y., Papo, N., and Shai, Y. (2006) "Endotoxin (Lipopolysaccharide) Neutralization by Innate Immunity Host-Defense Peptides: Peptide Properties and Plausible Modes of Action". J. Biol. Chem., 281, 1636-1643.

  22. Papo, N., Seger, D., Makovitzki, A., Kalchenko, V., Eshhar, Z., Degani, H., and Shai, Y. (2006) "Inhibition of tumor growth and elimination of multiple metastases in human prostate and breast xenografts by systemic inoculation of a host defense-like lytic peptide". Cancer Res., 66, 5371-5378.

  23. Mangoni, M. L., Papo, N., Saugar, J. M., Barra, D., Shai, Y., Simmaco, M., and Rivas, L. (2006) "Effect of natural L- to D-amino acid conversion on the organization, membrane binding, and biological function of the antimicrobial peptides bombinins H". Biochemistry, 45, 4266-4276.

  24. Makovitzki, A., Avrahami, D., and Shai, Y. (2006) "Ultrashort antibacterial and antifungal lipopeptides". Proc. Natl. Acad. Sci. U.S.A, 103, 15997-16002.

  25. Korazim, O., Sackett, K., and Shai, Y. (2006) "Functional and Structural Characterization of HIV-1 gp41 Ectodomain Regions in Phospholipid Membranes Suggests that the Fusion-active Conformation Is Extended". J. Mol. Biol., 364, 1103-1117.

  26. Carmieli, R., Papo, N., Zimmermann, H., Potapov, A., Shai, Y., and Goldfarb, D. (2006) "Utilizing ESEEM Spectroscopy to Locate the Position of Specific Regions of Membrane-Active Peptides within Model Membranes". Biophys. J., 90, 492-505.

  27. Wexler-Cohen, Y., Sackett, K. and Shai, Y. (2005) "The Role of the N-Terminal Heptad Repeat of HIV-1 in the Actual Lipid Mixing Step as Revealed by Its Substitution with Distant Coiled Coils". Biochemistry, 44, 5853-5861.

  28. Sal-Man, N., Gerber, D. and Shai, Y. (2005) "The identification of a minimal dimerization motif QxxS that enables homo- and hetero-association of TM helices in vivo". J. Biol. Chem., 280, 27449-27457.

  29. Sal-Man, N. and Shai, Y. (2005) "Arginine mutations within a transmembrane domain of Tar, an Escherichia coli aspartate receptor, can drive homodimer dissociation and heterodimer association in vivo". Biochem. J., 385, 29-36.

  30. Zelezetsky, I., Pacor, S., Pag, U., Papo, N., Shai, Y., Sahl, H.G. and Tossi, A. (2005) "Controlled alteration of the shape and conformational stability of alpha-helical cell-lytic peptides: effect on mode of action and cell specificity". Biochem. J., 390, 177-188.

  31. Sackett, K. and Shai, Y. (2005) "The HIV Fusion Peptide Adopts Intermolecular Parallel beta-Sheet Structure in Membranes when Stabilized by the Adjacent N-Terminal Heptad Repeat: A (13)C FTIR Study". J. Mol. Biol., 350, 790-805.

  32. Quintana, F.J., Gerber, D., Cohen, I.R. and shai, Y. (2005) "HIV-1 Fusion Peptide Targets the TCR and Inhibits Antigen-Specific T-Cell Activation". J. Clin. Invest., 115, 2149-2158.

  33. Papo, N. and Shai, Y. (2005) "A Molecular Mechanism for Lipopolysaccharide Protection of Gram-negative Bacteria from Antimicrobial Peptides". J. Biol. Chem., 280, 10378-10387.

  34. Malina, A. and Shai, Y. (2005) "Conjugation of fatty acids with different lengths modulates the antibacterial and antifungal activity of a cationic biologically inactive peptide". Biochem. J., in press.

  35. Makovitzki, A. and Shai, Y. (2005) "pH-Dependent Antifungal Lipopeptides and Their Plausible Mode of Action". Biochemistry, 44, 9775-9784.

  36. Gerber, D., Quintana, F.J., Bloch, I., Cohen, I.R. and Shai, Y. (2005) "D-enantiomer peptide of the TCR{alpha} transmembrane domain inhibits T-cell activation in vitro and in vivo". FASEB J., 19, 1190-1192.

  37. Sal-Man, N., Gerber, D. and Shai, Y. (2004) "Hetero-assembly Between All-l- and All-d-Amino Acid Transmembrane Domains: Forces Involved and Implication for Inactivation of Membrane Proteins". J. Mol. Biol., 344, 855-864.

  38. Gerber, D., Pritsker, M., Gunther-Ausborn, S., Johnson, B., Blumenthal, R. and Shai, Y. (2004) "Inhibition of HIV-1 Envelope Glycoprotein-mediated Cell Fusion by a DL-Amino Acid-containing Fusion Peptide: Possible Recognition of the Fusion Complex". J. Biol. Chem., 279, 48224-48230.

  39. Shnaper, S., Sackett, K., Gallo, S.A., Blumenthal, R. and Shai, Y. (2004) "The C- and the N-terminal regions of glycoprotein 41 ectodomain fuse membranes enriched and not enriched with cholesterol, respectively". J. Biol. Chem., 279, 18526-18534.

  40. Sal-Man, N., Gerber, D. and Shai, Y. (2004) "The Composition Rather than Position of Polar Residues (QxxS) Drives Aspartate Receptor Transmembrane Domain Dimerization in vivo". Biochemistry, 43, 2309-2313.

  41. Papo, N., Braunstein, A., Eshhar, Z. and Shai, Y. (2004) "Suppression of human prostate tumor growth in mice by a cytolytic D-, L-amino Acid Peptide: membrane lysis, increased necrosis, and inhibition of prostate-specific antigen secretion". Cancer Res., 64, 5779-5786.

  42. Papo, N. and Shai, Y. (2004) "Effect of Drastic Sequence Alteration and d-Amino Acid Incorporation on the Membrane Binding Behavior of Lytic Peptides". Biochemistry, 43, 6393-6403.

  43. Pag, U., Oedenkoven, M., Papo, N., Oren, Z., Shai, Y. and Sahl, H.G. (2004) "In vitro activity and mode of action of diastereomeric antimicrobial peptides against bacterial clinical isolates". J. Antimicrob. Chemother., 53, 230-239.

  44. Gerber, D., Sal-Man, N. and Shai, Y. (2004) "Structural Adaptation of the Glycophorin A Transmembrane Homodimer to d-Amino Acid Modifications" [Journal Cover Page]. J. Mol. Biol., 339, 243-250.

  45. Gerber, D., Sal-Man, N. and Shai, Y. (2004) "Two Motifs within a Transmembrane Domain, One for Homodimerization and the Other for Heterodimerization". J. Biol. Chem., 279, 21177-21182.

  46. Gallo, S.A., Sackett, K., Rawat, S.S., Shai, Y. and Blumenthal, R. (2004) "The stability of the intact envelope glycoproteins is a major determinant of sensitivity of HIV/SIV to peptidic fusion inhibitors". J. Mol. Biol., 340, 9-14.

  47. Braunstein, A., Papo, N. and Shai, Y. (2004) "In Vitro Activity and Potency of an Intravenously Injected Antimicrobial Peptide and Its DL Amino Acid Analog in Mice Infected with Bacteria. Antimicrob. Agents". Chemother., 48, 3127-3129.

  48. Avrahami, D. and Shai, Y. (2004) "A New Group of Antifungal and Antibacterial Lipopeptides Derived from Non-membrane Active Peptides Conjugated to Palmitic Acid". J. Biol. Chem., 279, 12277-12285.

  49. Sackett, K. and Shai, Y. (2003) "How structure correlates to function for membrane associated HIV-1 gp41 constructs corresponding to the N-terminal half of the ectodomain". J. Mol. Biol., 333, 47-58.

  50. Peisajovich, S.G., Gallo, S.A., Blumenthal, R. and Shai, Y. (2003) "C-terminal Octylation Rescues an Inactive T20 Mutant: implications for the mechanism of hiv/simian immunodeficiency virus-induced membrane fusion". J. Biol. Chem., 278, 21012-21017.

  51. Peisajovich, S.G., Blank, L., Epand, R.F., Epand, R.M. and Shai, Y. (2003) "On the Interaction Between gp41 and Membranes: The Immunodominant Loop Stabilizes gp41 Helical Hairpin Conformation". J. Mol. Biol., 326, 1489-1501.

  52. Papo, N. and Shai, Y. (2003) "New lytic peptides based on the D,L-amphipathic helix motif preferentially kill tumor cells compared to normal cells". Biochemistry, 42, 9346-9354.

  53. Papo, N., Shahar, M., Eisenbach, L. and Shai, Y. (2003) "A novel lytic peptide composed of DL-amino acids selectively kills cancer cells in culture and in mice". J. Biol. Chem., 278, 21018-21023.

  54. Papo, N. and Shai, Y. (2003) "Exploring Peptide Membrane Interaction Using Surface Plasmon Resonance: Differentiation between Pore Formation versus Membrane Disruption by Lytic Peptides". Biochemistry, 42, 458-466.

  55. Mangoni, M.L., Papo, N., Mignogna, G., Andreu, D., Shai, Y., Barra, D. and Simmaco, M. (2003) "Ranacyclins, a new family of short cyclic antimicrobial peptides: biological function, mode of action, and parameters involved in target specificity". Biochemistry, 42, 14023-14035.

  56. Avrahami, D. and Shai, Y. (2003) "Bestowing Antifungal and Antibacterial Activities by Lipophilic Acid Conjugation to d,l-Amino Acid-Containing Antimicrobial Peptides: A Plausible Mode of Action". Biochemistry, 42, 14946-14956.

  57. Sal-Man, N., Oren, Z. and Shai, Y. (2002) "Preassembly of Membrane-Active Peptides Is an Important Factor in Their Selectivity toward Target Cells". Biochemistry, 41, 11921-11930.

  58. Gerber, D. and Shai, Y. (2002) "Chirality-Independent Protein-Protein Recognition Between Transmembrane Domains In Vivo" [Journal Cover Page]. J. Mol. Biol., 322, 491-495.

  59. Peisajovich, S.G., Epand, R.F., Epand, R.M. and Shai, Y. (2002) "Sendai virus N-terminal fusion peptide consists of two similar repeats, both of which contribute to membrane fusion". Eur. J. Biochem., 269, 4342-4350.

  60. Papo, N., Oren, Z., Ulrike, P., Sahl, H.G. and Shai, Y. (2002) “The Consequence of Sequence Alteration of an Amphipathic α-Helical Antimicrobial Peptide and its Diastereomers”. J. Biol. Chem., 277, 33913-33921.

  61. Oren, Z., Ramesh, J., Avrahami, D., Suryaprakash, N., Shai, Y. and Jelinek, R. (2002) “Structures and Mode of Membrane Interaction of a Short α-Helical Lytic Peptide and Its Diastereomer Determined by NMR, FTIR, and Fluorescence Spectroscopy”. Eur. J. Biochem., 269, 3869-3880.

  62. Sackett, K. and Shai, Y. (2002) "The HIV-1 gp41 N-Terminal Heptad Repeat Plays an Essential Role in Membrane Fusion". Biochemistry, 2002, 41, 4678-4685.

  63. Avrahami, D. and Shai, Y. (2002) "Conjugation of a magainin analog with lipophilic acids controls hydrophobicity, solution assembly and cell-selectivity". Biochemistry, 41, 2254-2263.

  64. Peisajovich, S. G. and Shai, Y. (2002) "High similarity between reverse-oriented sequences from HIV and foamy virus envelope glycoproteins". AIDS Res. Hum. Retroviruses, 18309-18312.

  65. Avrahami, D., Oren, Z. and Shai, Y. (2001) "Effect of multiple aliphatic amino acids substitutions on the structure, function, and mode of action of diastereomeric membrane active peptides". Biochemistry, 40, 12591-12603.

  66. Gerber, D. and Shai, Y. (2001) "In vivo detection of hetero-association of glycophorin-A and its mutants within the membrane". J. Biol. Chem., 276, 31229-31232.

  67. Peisajovich, S.G. and Shai, Y. (2001) "SIV Gp41 Binds to Membranes both in the Monomeric and Trimeric States: Consequences for the Neuropathology and Inhibition of HIV Infection". J. Mol. Biol., 311, 249-254.

  68. Samuel, O. and Shai, Y. (2001) "Participation of Two Fusion Peptides in Measles Virus-Induced Membrane Fusion: Emerging Similarity with Other Paramyxoviruses". Biochemistry, 40, 1340-1349.

  69. Kliger, Y., Gallo, S.A., Peisajovich, S.G., Munoz-Barroso, I., Avkin, S., Blumenthal, R. and Shai, Y. (2001) "Mode of Action of an Antiviral Peptide from HIV-1. Inhibition at A Post-Lipid Mixing Stage". J. Biol. Chem., 276, 1391-1397.

  70. Unger, T., Oren, Z. and Shai, Y. (2001) "The Effect of Cyclization of Magainin 2 and Melittin Analogs on Structure, Function and Model Membrane Interactions: Implication to their Mode of Action". Biochemistry, 40, 6388-6397.

  71. Gerber, D. and Shai, Y. (2000) "Insertion and Organization Within Membranes of the {delta}-endotoxin Pore-Forming Domain, Helix 4-Loop-Helix 5, and Inhibition of its Activity by a Mutant Helix 4 Peptide". J. Biol. Chem., 275, 23602-23607.

  72. Hetru, C., Letellier, L., Oren, Z., Hoffmann, J.A. and Shai, Y. (2000) "Androctonin, a hydrophilic disulphide-bridged non-haemolytic anti- microbial peptide: a plausible mode of action". Biochem. J., 345, 653-664.

  73. Ghosh, J.K., Peisajovich, S.G. and Shai, Y. (2000) "Sendai Virus Internal Fusion Peptide: Structural and Functional Characterization and a Plausible Mode of Viral Entry Inhibition". Biochemistry, 39, 11581-11592.

  74. Kliger, Y. and Shai, Y. (2000) "Inhibition of HIV-1 Entry Before gp41 Folds Into its Fusion-active Conformation". J. Mol. Biol., 295, 163-168.

  75. Kliger, Y., Peisajovich, S.G., Blumenthal, R. and Shai, Y. (2000) "Membrane-induced Conformational Change During the Activation of HIV-1 gp41" [Journal Cover Page]. J. Mol. Biol., 301, 905-914.

  76. Peri, I., Mamrud-Brains, H., Rodin, S., Krizhanovsky, V., Shai, Y., Nir, S. and Naim, M. (2000) "Rapid entry of bitter and sweet tastants into liposomes and taste cells: implications for signal transduction". Am. J. Physiol., 278, C17-25.

  77. Oren, Z. and Shai, Y. (2000) "Cyclization of a Cytolytic Amphipathic alpha-Helical Peptide and Its Diastereomer: Effect on Structure, Interaction with Model Membranes, and Biological Function". Biochemistry, 39, 6103-6114.

  78. Peisajovich, S.G., Epand, R.F., Pritsker, M., Shai, Y. and Epand, R.M. (2000) "The Polar Region Consecutive to the HIV Fusion Peptide Participates in Membrane Fusion". Biochemistry, 39, 1826-1833.

  79. Peisajovich, S.G., Samuel, O. and Shai, Y. (2000) "Paramyxovirus F1 Protein Has Two Fusion Peptides: Implications for the Mechanism of Membrane Fusion" [Journal Cover Page]. . J. Mol. Biol., 296, 1353-1365.

  80. Sharon, M., Oren, Z., Shai, Y. and Anglister, J. (1999) "2D-NMR and ATR-FTIR Study of the Structure of a Cell-Selective Diastereomer of Melittin and Its Orientation in Phospholipids". Biochemistry, 38, 15305-15316.

  81. Oren, Z., Lerman, J.C., Gudmundsson, G.H., Agerberth, B. and Shai, Y. (1999) "Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis for its non- cell-selective activity". Biochem. J., 341, 501-513.

  82. Pritsker, M., Rucker, J., Hoffman, T.L., Doms, R.W. and Shai, Y. (1999) "Effect of nonpolar substitutions of the conserved Phe11 in the fusion peptide of HIV-1 gp41 on its function, structure, and organization in membranes". Biochemistry, 38, 11359-11371.

  83. La Rocca, P., Shai, Y. and Sansom, M.S. (1999) "Peptide-bilayer interactions: simulations of dermaseptin B, an antimicrobial peptide". Biophys. Chem., 76, 145-159.

  84. Hong, J., Oren, Z. and Shai, Y. (1999) "Structure and Organization of Hemolytic and Nonhemolytic Diastereomers of Antimicrobial Peptides in Membranes". Biochemistry, 38, 16963-16973.

  85. Ben Efraim, I., Kliger, Y., Hermesh, C. and Shai, Y. (1999) "Membrane-induced step in the activation of Sendai virus fusion protein". J. Mol. Biol., 285, 609-625.

  86. Ghosh, J.K. and Shai, Y. (1999) "Direct Evidence that the N-Terminal Heptad Repeat of Sendai Virus Fusion Protein Participates in Membrane Fusion". J. Mol. Biol., 292, 531-546.

  87. Oren, Z., Hong, J. and Shai, Y. (1999) "A comparative study on the structure and function of a cytolytic alpha- helical peptide and its antimicrobial beta-sheet diastereomer". Eur. J. Biochem., 259, 360-369.

  88. Gazit, E., La Rocca, P., Sansom, M.S. and Shai, Y. (1998) "The structure and organization within the membrane of the helices composing the pore-forming domain of Bacillus thuringiensis delta- endotoxin are consistent with an "umbrella-like" structure of the pore". Proc. Natl. Acad. Sci. USA, 95, 12289-12294.

  89. Ghosh, J.K., Peisajovich, S.G., Ovadia, M. and Shai, Y. (1998) "Structure-function study of a heptad repeat positioned near the transmembrane domain of Sendai virus fusion protein which blocks virus- cell fusion". J. Biol. Chem., 273, 27182-27190.

  90. Ghosh, J.K. and Shai, Y. (1998) "A peptide derived from a conserved domain of Sendai virus fusion protein inhibits virus-cell fusion. A plausible mode of action". J. Biol. Chem., 273, 7252-7259.

  91. Abu-Raya, S., Bloch-Shilderman, E., Shohami, E., Trembovler, V., Shai, Y., Weidenfeld, J., Yedgar, S., Gutman, Y. and Lazarovici, P. (1998) "Pardaxin, a new pharmacological tool to stimulate the arachidonic acid cascade in PC12 cells". J. Pharmacol. Exp. Ther., 287, 889-896.

  92. Pritsker, M., Jones, P., Blumenthal, R. and Shai, Y. (1998) "A synthetic all D-amino acid peptide corresponding to the N-terminal sequence of HIV-1 gp41 recognizes the wild-type fusion peptide in the membrane and inhibits HIV-1 envelope glycoprotein-mediated cell fusion". Proc. Natl. Acad. Sci. USA, 95, 7287-7292.

  93. Ghosh, J.K., Shaool, D., Guillaud, P., Ciceron, L., Mazier, D., Kustanovich, I., Shai, Y. and Mor, A. (1997) "Selective cytotoxicity of dermaseptin S3 toward intraerythrocytic Plasmodium falciparum and the underlying molecular basis". J. Biol. Chem., 272, 31609-31616.

  94. Gazit, E., Burshtein, N., Ellar, D.J., Sawyer, T. and Shai, Y. (1997) "Bacillus thuringiensis cytolytic toxin associates specifically with its synthetic helices A and C in the membrane bound state. Implications for the assembly of oligomeric transmembrane pores". Biochemistry, 36, 15546-15554.

  95. Ben Efraim, I. and Shai, Y. (1997) "The structure and organization of synthetic putative membranous segments of ROMK1 channel in phospholipid membranes". Biophys. J., 72, 85-96.

  96. Kliger, Y., Aharoni, A., Rapaport, D., Jones, P., Blumenthal, R. and Shai, Y. (1997) "Fusion peptides derived from the HIV type 1 glycoprotein 41 associate within phospholipid membranes and inhibit cell-cell Fusion. Structure- function study". J. Biol. Chem., 272, 13496-13505.

  97. Kliger, Y. and Shai, Y. (1997) "A leucine zipper-like sequence from the cytoplasmic tail of the HIV-1 envelope glycoprotein binds and perturbs lipid bilayers". Biochemistry, 36, 5157-5169.

  98. Ghosh, J.K., Ovadia, M. and Shai, Y. (1997) "A leucine zipper motif in the ectodomain of Sendai virus fusion protein assembles in solution and in membranes and specifically binds biologically-active peptides and the virus". Biochemistry, 36, 15451-15462.

  99. Oren, Z. and Shai, Y. (1997) "Selective lysis of bacteria but not mammalian cells by diastereomers of melittin: structure-function study". Biochemistry, 36, 1826-1835.

  100. Oren, Z., Hong, J. and Shai, Y. (1997) "A repertoire of novel antibacterial diastereomeric peptides with selective cytolytic activity". J. Biol. Chem., 272, 14643-14649.

  101. Rapaport, D., Peled, R., Nir, S. and Shai, Y. (1996) "Reversible surface aggregation in pore formation by pardaxin". Biophys. J., 70, 2502-2512.

  102. Peled-Zehavi, H., Arkin, I.T., Engelman, D.M. and Shai, Y. (1996) "Coassembly of synthetic segments of shaker K+ channel within phospholipid membranes". Biochemistry, 35, 6828-6838.

  103. Shai, Y. and Oren, Z. (1996) "Diastereoisomers of cytolysins, a novel class of potent antibacterial peptides". J. Biol. Chem., 271, 7305-7308.

  104. Vouldoukis, I., Shai, Y., Nicolas, P. and Mor, A. (1996) "Broad spectrum antibiotic activity of the skin-PYY". FEBS Lett., 380, 237-240.

  105. Gazit, E., Miller, I.R., Biggin, P.C., Sansom, M.S.P. and Shai, Y. (1996) "Structure and orientation of the mammalian antibacterial peptide cecropin P1 within phospholipid membranes". J. Mol. Biol., 258, 860-870.

  106. Ben Efraim, I. and Shai, Y. (1996) "Secondary structure, membrane localization, and coassembly within phospholipid membranes of synthetic segments derived from the N- and C- termini regions of the ROMK1 K+ channel". Protein Sci., 5, 2287-2297.

  107. Oren, Z. and Shai, Y. (1996) "A class of highly potent antibacterial peptides derived from pardaxin, a pore-forming peptide isolated from Moses sole fish Pardachirus marmoratus". Eur. J. Biochem., 237, 303-310.

  108. Ben Efraim, I., Shai, Y. and Attali, B. (1996) "Cytoplasmic and extracellular IsK peptides activate endogenous K+ and Cl- channels in Xenopus oocytes. Evidence for regulatory function". J. Biol. Chem., 271, 8768-8771.

  109. Rapaport, D., Ovadia, M. and Shai, Y. (1995) "A synthetic peptide corresponding to a conserved heptad repeat domain is a potent inhibitor of Sendai virus-cell fusion: an emerging similarity with functional domains of other viruses". EMBO J., 14, 5524-5531.

  110. Gazit, E., Boman, A., Boman, H.G. and Shai, Y. (1995) "Interaction of the mammalian antibacterial peptide cecropin P1 with phospholipid vesicles". Biochemistry, 34, 11479-11488.

  111. Pouny, Y. and Shai, Y. (1995) "Synthetic peptides corresponding to the four P regions of Electrophorus electricus Na+ channel: interaction with and organization in model phospholipid membranes". Biochemistry, 34, 7712-7721.

  112. Gazit, E. and Shai, Y. (1995) "The assembly and organization of the alpha 5 and alpha 7 helices from the pore-forming domain of Bacillus thuringiensis delta-endotoxin. Relevance to a functional model". J. Biol. Chem., 270, 2571-2578.

  113. Peled, H. and Shai, Y. (1994) "Synthetic S-2 and H-5 segments of the Shaker K+ channel: secondary structure, membrane interaction, and assembly within phospholipid membranes". Biochemistry, 33, 7211-7219.

  114. Rapaport, D. and Shai, Y. (1994) "Interaction of fluorescently labeled analogues of the amino-terminal fusion peptide of Sendai virus with phospholipid membranes". J. Biol. Chem., 269, 15124-15131.

  115. Strahilevitz, J., Mor, A., Nicolas, P. and Shai, Y. (1994) "Spectrum of antimicrobial activity and assembly of dermaseptin-b and its precursor form in phospholipid membranes". Biochemistry, 33, 10951-10960.

  116. Ben Efraim, I., Strahilevitz, J., Bach, D. and Shai, Y. (1994) "Secondary structure and membrane localization of synthetic segments and a truncated form of the IsK (minK) protein". Biochemistry, 33, 6966-6973.

  117. Gazit, E., Lee, W.J., Brey, P.T. and Shai, Y. (1994) "Mode of action of the antibacterial cecropin B2: a spectrofluorometric study". Biochemistry, 33, 10681-10692.

  118. Gazit, E., Bach, D., Kerr, I.D., Sansom, M.S., Chejanovsky, N. and Shai, Y. (1994) "The alpha-5 segment of Bacillus thuringiensis delta-endotoxin: in vitro activity, ion channel formation and molecular modelling". Biochem. J., 304, 895-902.

  119. Rapaport, D., Nir, S. and Shai, Y. (1994) "Capacities of pardaxin analogues to induce fusion and leakage of negatively charged phospholipid vesicles are not necessarily correlated". Biochemistry, 33, 12615-12624.

  120. Gazit, E. and Shai, Y. (1993) "Structural and functional characterization of the alpha 5 segment of Bacillus thuringiensis delta-endotoxin". Biochemistry, 32, 3429-3436.

  121. Ben Efraim, I., Bach, D. and Shai, Y. (1993) "Spectroscopic and functional characterization of the putative transmembrane segment of the minK potassium channel". Biochemistry, 32, 2371-2377.

  122. Rapaport, D., Hague, G.R., Pouny, Y. and Shai, Y. (1993) "pH- and ionic strength-dependent fusion of phospholipid vesicles induced by pardaxin analogues or by mixtures of charge-reversed peptides". Biochemistry, 32, 3291-3297.

  123. Peled, H. and Shai, Y. (1993) "Membrane interaction and self-assembly within phospholipid membranes of synthetic segments corresponding to the H-5 region of the shaker K+ channel". Biochemistry, 32, 7879-7885.

  124. Eshel, Y., Shai, Y., Vorherr, T., Carafoli, E. and Salomon, Y. (1993) "Synthetic peptides corresponding to the calmodulin-binding domains of skeletal muscle myosin light chain kinase and human erythrocyte Ca2+ pump interact with and permeabilize liposomes and cell membranes". Biochemistry, 32, 6721-6728.

  125. Gazit, E. and Shai, Y. (1993) "Structural characterization, membrane interaction, and specific assembly within phospholipid membranes of hydrophobic segments from Bacillus thuringiensis var. israelensis cytolytic toxin". Biochemistry, 32, 12363-12371.

  126. Rapaport, D., Danin, M., Gazit, E. and Shai, Y. (1992) "Membrane interactions of the sodium channel S4 segment and its fluorescently-labeled analogues". Biochemistry, 31, 8868-8875.

  127. Pouny, Y., Rapaport, D., Mor, A., Nicolas, P. and Shai, Y. (1992) "Interaction of antimicrobial dermaseptin and its fluorescently labeled analogues with phospholipid membranes". Biochemistry, 31, 12416-12423.

  128. Pouny, Y. and Shai, Y. (1992) "Interaction of D-amino acid incorporated analogues of pardaxin with membranes". Biochemistry, 31, 9482-9490.

  129. Rapaport, D. and Shai, Y. (1992) "Aggregation and organization of pardaxin in phospholipid membranes. A fluorescence energy transfer study". J. Biol. Chem., 267, 6502-6509.

  130. Eastman, R.C., Carson, R.E., Jacobson, K.A., Shai, Y., Channing, M.A., Dunn, B.B., Bacher, J.D., Baas, E., Jones, E., Kirk, K.L. and et al. (1992) "In vivo imaging of insulin receptors in monkeys using 18F-labeled insulin and positron emission tomography". Diabetes, 41, 855-860.

  131. Rapaport, D. and Shai, Y. (1991) "Interaction of fluorescently labeled pardaxin and its analogues with lipid bilayers". J. Biol. Chem., 266, 23769-23775.

  132. Shai, Y., Hadari, Y.R. and Finkels, A. (1991) "pH-dependent pore formation properties of pardaxin analogues". J. Biol. Chem., 266, 22346-22354.

  133. Shai, Y., Bach, D. and Yanovsky, A. (1990) "Channel formation properties of synthetic pardaxin and analogues". J. Biol. Chem., 265, 20202-20209.

  134. Shai, Y., Kirk, K.L., Channing, M.A., Dunn, B.B., Lesniak, M.A., Eastman, R.C., Finn, R.D., Roth, J. and Jacobson, K.A. (1989) "18F-labeled insulin: a prosthetic group methodology for incorporation of a positron emitter into peptides and proteins". Biochemistry, 28, 4801-4806.

  135. Shai, Y., Brunck, T.K. and Chaiken, I.M. (1989) "Antisense peptide recognition of sense peptides: sequence simplification and evaluation of forces underlying the interaction". Biochemistry, 28, 8804-8811.

  136. Shai, Y., Fox, J., Caratsch, C., Shih, Y.L., Edwards, C. and Lazarovici, P. (1988) "Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity". FEBS Lett., 242, 161-166.

  137. Shai, Y., Flashner, M. and Chaiken, I.M. (1987) "Anti-sense peptide recognition of sense peptides: direct quantitative characterization with the ribonuclease S-peptide system using analytical high-performance affinity chromatography". Biochemistry, 26, 669-675.

  138. Shai, Y. and Warshawsky, A. (1985) "Stability constants of oximes of 4-substituted 2-acetylphenol". Isr. J. Chem., 25, 233-235.

  139. Shai, Y., Jacobson, K. and Patchornik, A. (1985) "A mediator methodology for peptide and nucleotide synthesis using dipolymeric system". J. Am. Chem. Soc., 107, 4249-4252.

  140. Warshawsky, A., Deshe, A. and Shai, Y. (1985) "Transition metal chelating agents from 3,4-disubstituted benzyl halides". Isr. J. Chem., 26, 48-55.

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Invited Reviews in Refereed Journals

  1. Shai, Y. (1994) "Pardaxin: channel formation by a shark repellant peptide from fish". Toxicology, 87, 109-129.

  2. Shai, Y. (1995) "Molecular recognition between membrane-spanning polypeptides". Trends. Biochem. Sci. (TIBS), 20, 460-464.

  3. Epand, R.M., Shai, Y., Segrest, J.P. and Anantharamaiah, G.M. (1995) "Mechanisms for the modulation of membrane bilayer properties by amphipathic helical peptides". Biopolymers, 37, 319-338.

  4. Durell, S.R., Martin, I., Ruysschaert, J.M., Shai, Y. and Blumenthal, R. (1997) "What studies of fusion peptides tell us about viral envelope glycoprotein-mediated membrane fusion" (review). Mol. Membr. Biol., 14, 97-112.

  5. Shai, Y. (1997) "Topology and organization of ion channels: A spectroscopic study using a synthetic peptide approach". Chemtracts-Biochem and Molecular Biology, 10, 207-224.

  6. Oren, Z. and Shai, Y. (1998) "Mode of action of linear amphipathic alpha-helical antimicrobial peptides". Biopolymers, 47, 451-463.

  7. Shai, Y. (1999) "Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides". Biochem. Biophys. Acta., 1462, 55-70.

  8. Shai, Y. (2000) "Functional Domains within Fusion Proteins: Prospectives for Development of Peptide Inhibitors of Viral-Cell Fusion". Bioscience Reports, 20, 535-555.

  9. Aronson, A.I. and Shai, Y. (2001) "Why Bacillus thuringiensis insecticidal toxins are so effective: unique features of their mode of action". FEMS Microbiol. Lett., 195, 1-8.

  10. Shai, Y. and Oren, Z. (2001) "From 'carpet' mechanism to de-novo designed diastereomeric cell-selective antimicrobial peptides". Peptides, 22, 1629-1641.

  11. Shai, Y. (2001) "Molecular recognitioin within the membrane milieu: Implication for the structure and function of membrane proteins". J. Membrane Biol., 182, 91-104.

  12. Peisajovich S.J. and Shai, Y. (2002) "New Insights into the Mechanism of Virus-Induced Membrane Fusion Trends". Biochem. Sci. (TIBS), 2002, 27, 183-90.

  13. Shai, Y. (2002) "From Innate Immunity to de-Novo Designed Antimicrobial Peptides". Curr. Pharmaceutical Design, 2002, 8, 715-25.

  14. Papo, N. and Shai, Y. (2003) "Can we predict biological activity of antimicrobial peptides from their interactions with model phospholipid membranes?" Peptides, 24, 1693-1703.

  15. Shai, Y. Guest Editor, special Issue on Membrane Fusion. BBA., 2003.

  16. Peisajovich, S. G. & Shai, Y. (2003) "Viral fusion proteins: multiple regions contribute to membrane fusion". Biochim. Biophys. Acta 1614, 122-129.

  17. Papo, N. and Shai, Y. (2005) "Host defense peptides as new weapons in cancer treatment". Cell. Mol. Life Sci., 62, 784-790.

  18. Rosenfeld, Y., and Shai, Y. (2006) "Lipopolysaccharide (Endotoxin)-host defense antibacterial peptides interactions: Role in bacterial resistance and prevention of sepsis". Biochim. Biophys. Acta, in press.

  19. Shai, Y., Makovitzki, A., and Avrahami, D. (2006) "Host Defense Peptides and Lipopeptides: Modes of Action and Potential Candidates for the Treatment of Bacterial and Fungal Infections". Current Protein & Peptide Science (CPPS), in press.

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Chapters in Books

  1. Patchornik, A., Nov, E., Jacobson, A. and Shai, Y. (1986) “Polymeric transfer reagents for organic synthesis with self control: towards automation in organic synthesis”. In: Polymeric Reagents and Catalysts (Ford, W.T., ed.). pp. 231-246. ACS, Washington.

  2. Shai, Y, Flashner, M. and Chaiken, I.M. (1987). “Anti-sense peptides designing recognition molecules”. In: Protein Structure and Design, Vol. 69 (Oxender, E., ed.) pp. 439-452. New York, Alan R. Lyss.

  3. Shai, Y. “Mechanism of membrane permeation: Pore formation by the shark repellent neurotoxin pardaxin, versus membrane disruption by antibacterial polypeptides” In: Biochemical Aspects of Marine Pharmacology, Lazarovici, P., Spira, M. E., Zlotkin, E. pp. 175-186. Alaken, Inc. Fort Collins, Colorado, USA.

  4. Chaiken, I.M., Ando, S., Shai, Y., Fassina, G. and Xun, L. (1988) “Synthetic peptides and the desing of peptide and protein recognition surfaces”. In: Macromolecules Biorecognition - Principles and Methods, (Chaiken, I.M., Chaincone, E., Fontana, A., Neir, P., eds.) pp. 29-50. Clifton, NJ, The Humana Press.

  5. Chaiken, I.M., Shai, Y., Fassina, G. and Caliceti, P. (1988) “Evaluation of analytical affinity chromatography - Desing and biotechnological uses of molecular recognition surfaces”. Makromol. Chem. Macromol. Symp., 17, 269-279.

  6. Lazarovici, P., Primor, N., Caratasch, C., Lelkes, P.I., Fox, J., Shai, Y., McPhie, P. and Shih, Y.L. and Edwards, C. (1988) “Action of paradaxin, a new presynaptic excitatory neurotoxin with ionophore activity on neuromuscular junction, synaptozomes and pheochromocytoma PC 12 cells”. In: Neurotoxins as Tools in Neurochemistry, (Holly, O., ed.) pp. 219-240.

  7. Chaiken, I.M., Fassina, G. and Shai, Y. (1989) “Analytical affinity chromatography, anti-sense peptides, and the synthetic design of recognition molecules". In: Immobilized Ligands, (Hutchens, T.W., ed.) Alan Lyss, NY.

  8. Chaiken, I.M., Ando, S., Fassina, G. and Shai, Y. “Sequence simplification and randomization and the design of peptide recognition surfaces”. In: Peptide Structure and Function, Proc. of the 10th American Peptide Symposium, Pierce, ILL.

  9. Lazarovici, P., Primor, N., Genaro, J., Fox, J., Shai. Y., Lelkes, P. I., Caratsch, C.G., Raghunathan, G., Guy, H.R., Shih, Y. L. and Edwards, C. (1990) “Origin, chemistry and mechanism of action of paradaxin, a shark repellent, presynaptic excitatory - ionophore polypeptide”. In: Marine Toxins; Origins, Structure and Pharmacology, (Holl, S., & Strichartz, J. ed.) pp. 347-364. A.C.S. series 418, Washington, DC.

  10. Gazit, E. and Shai, Y. (1992) “Membrane Interaction and Hemolytic Activity of a-5 helix of d-endotoxin”. In: Recent Advances in Molecular and Biochemical Research On Proteins, (Wei, W. -H., Chen, C. -S. & Su, J. -C. eds.) pp. 145-153. World Scientific Publishing Co. NJ.

  11. Gazit, E. and Shai, Y. (1994) “On the Mechanism of Action of the Antibacterial Cecropin B2” in: Antibacterial peptides. Ciba Foundation Symposium 186, John Wiley & Sons.

  12. Cosette, P., Helluin, O., Breed, J, Pouny, Y., Shai, Y., Sanson, M. S. P. and Duclohier, H. (1997) “Functional, conformational and molecular modelling studies of voltage-sensitivity and selectivity of synthetic peptides derived from ion channels”. pp. 41-62. (Eds. Tori and Conti), Plenum Press, New York.

  13. Shai, Y. (1996). “Mechanism of membrane permeation: Pore formation by the shark repellent neurotoxin pardaxin, versus membrane permeation by antibacterial polypeptides”. In: Biochemical Aspects of Marine Pharmacology, (Eds. Lazarovici, P., Spira, M.E. and Zlotkin, E.) pp.175-186. Alaken Inc. Fort Collins, Colorado, USA.

  14. Shai, Y. (1997) “Mode of action of anitmicrobial peptides". In: Molecular Mechanisms of Immune Responses in Insects, (Eds. Brey, P. T. and Hultmark, D.) pp. 111-134. Chapman & Hall, London.

  15. Shai, Y. (2000) “Bacterial membrane as a target for a novel class of diastereomers of cytolytic peptides”. In: Membrane Structure in Disease and Drug Therapy. (Ed. Zimmer. G.) pp 27-44. Marcel Becker Inc. New York.

  16. Shai, Y. (2001) “Molecular recognition in the membrane: Role in the folding of membrane proteins". In: Self-assembling Peptide Systems in Biology, Medicine and Engineering, (Eds. Aggeli, A., Boden, N. and Zhang, S.). pp. 273-294. Kluwer Academic Publishers, Dordrecht, The Netherlands.

  17. Oren, Z, and Shai, Y. (2001) “Molecular mechanism of cell selectivity by linear amphipathic α-helical peptides and diasteriomeric antimicrobial peptides”. In: Development of Novel Antimicrobial Agents: Emerging strategies, (Ed. Lohner, K.) pp.183-204. Horizen Scientific Press, Norfolk, England.

  18. Gerber, D. & Shai, Y. (2002). “Delta-Endotoxin Mode of Action”. In: Pore-forming Peptides and Protein Toxins, (Eds. Menestrina, G. & Lazarovici, P.). Series on "Cellular and Molecular Mechanisms of Toxin Action". Harwood Academic Publishers. In press.

  19. Shai, Y. (2004). “Mechanism of Membrane Permeation by Antimicrobial Peptides”. In: Protein – lipid Interactions, (ed. Tamm, LK). Wiley-VCH Publishers.

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Patents

  1. Shai, Y., and Oren, Z. (1997) "Antipathogenic peptides and compositions comprising them".

  2. Shai, Y., Oren, Z., Eyal, N., and Shachar, M. (2001) "Diastereomeric peptides and pharmaceutical compositions comprising them".

  3. Shai, Y. (2003) "Diasteriomeric peptides useful as inhibitors of membrane protein assembly".

  4. Shai, Y., and Avrahami, D. (2003) "Antimicrobial and anticancer lipopeptides".

  5. Shai, Y., Cohen, I. R., Quintana, F., and Gerber, D. (2005) "HIV-1 gp41 fusion peptides for immunomodulation".

  6. Shai, Y., and Makovitzki, A. (2005) "Histidine containing diastereomers (d,l-amino acids) of lytic peptides for cancer treatment".