Selected Publications

• Tokuriki N, Tawfik DS. “Protein dynamism and evolvability.” Science, 2009 Apr 10;324(5924):203-7.

• Goldsmith M, Tawfik DS. “Potential role of phenotypic mutations in the evolution of protein expression and stability.” Proc Natl Acad Sci U S A., 2009 Apr 14;106(15):6197-202.

• Tokuriki N, Oldfield CJ, Uversky VN, Berezovsky IN, Tawfik DS. ”Do viral proteins possess unique biophysical features?” Trends Biochem Sci., 2009 Feb;34(2):53-9.

• Gupta RD, Tawfik DS. “Directed enzyme evolution via small and effective neutral drift libraries.” Nat Methods., 2008 Nov;5(11):939-42.

• Bershtein S, Tawfik DS. “Ohno's model revisited: measuring the frequency of potentially adaptive mutations under various mutational drifts.” Mol Biol Evol., 2008 Nov;25(11):2311-8.

• Shimon Bershtein, Korina Goldin, Tawfik DS. "Intense Neutral Drifts Yield Robust and Evolvable Consensus Proteins." J Mol Biol., June 2008;379(5):1029–1044.

• Röthlisberger D, Khersonsky O, Wollacott AM, Jiang L, DeChancie J, Betker J, Gallaher JL, Althoff EA, Zanghellini A, Dym O, Albeck S, Houk KN, Tawfik DS, Baker D. "Kemp elimination catalysts by computational enzyme design." Nature, 2008 May 8;453(7192):190-195.

• Bershtein S, Tawfik DS. "Advances in laboratory evolution of enzymes." Curr Opin Chem Biol., 2008 Apr;12(2):151-158.

• Tokuriki N, Stricher F, Serrano L, Tawfik DS. "How protein stability and new functions trade off." PLoS Comput Biol., 2008 Feb 29;4(2):2-7.

• Goldsmith M, Kiss C, Bradbury AR, Tawfik DS. "Avoiding and controlling double transformation artifacts." Protein Eng Des Sel., 2007 Jul;20(7):315-318.

• Peisajovich SG, Tawfik DS. "Protein engineers turned evolutionists." Nat. Methods Dec 2007;4(12):991-994.

• Amitai G, Devi Gupta R, Tawfik DS. "Latent evolutionary potentials under the neutral mutational drift of an enzyme." HFSP Journal, May 2007;1(1):67-78.

• Tokuriki N, Stricher F, Schymkowitz J, Serrano L, Tawfik DS. "The stability effects of protein mutations appear to be universally distributed." J. Mol. Biol. 2007 Jun 22;369(5):1318-1332.

• Gaidukov L, Tawfik DS. "The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activity." J. Lipid Res., 2007 Jul;48(7):1637-1646.

• Herman A, Tawfik DS. "Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries." Protein Eng. Des. Sel. 2007 May;20(5):219-226.

• Bershtein S, Segal M, Bekerman R, Tokuriki N, Tawfik DS. "Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein." Nature, 2006 Dec 14;444(7121):929-932.

• Yadid I, Tawfik DS. "Reconstruction of functional β-propeller lectins via homo-oligomeric assembly of shorter fragments." J Mol Biol., 2007 Jan 5;365(1):10-17.

• Afriat L, Roodveldt C, Manco G, Tawfik DS. "The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase." Biochemistry, 2006 Nov 21;45(46):13677-13686.

• Khersonsky O, Roodveldt C, Tawfik DS. (2006) "Enzyme promiscuity: evolutionary and mechanistic aspects." Curr Opin Chem Biol., 2006 Oct;10(5):498-508.

• Griffiths AD, Tawfik DS. (2006) "Miniaturising the laboratory in emulsion droplets." Trends Biotechnol., 2006 Jul 13;30(10):1-8..

• Miller OJ, Bernath K, Agresti JJ, Amitai G, Kelly BT, Mastrobattista E, Taly V, Magdassi S, Tawfik DS, Griffiths AD. (2006) "Directed evolution by in vitro compartmentalization." Nat Methods., 2006 Jul;3(7):561-570.

• Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD. (2006) "Amplification of complex gene libraries by emulsion PCR." Nat Methods, 2006 Jul;3(7):545-550.

• Amitai G, Gaidukov L, Adani R, Yishay S, Yacov G, Kushnir M, Teitlboim S, Lindenbaum M, Bel P, Khersonsky O, Tawfik DS, Meshulam H. (2006) "Enhanced stereoselective hydrolysis of toxic organophosphates by directly evolved variants of mammalian serum paraoxonase." FEBS J., 2006 May;273(9):1906-1919.

• Tawfik DS. (2006) "Loop grafting and the origins of enzyme species." Science. 2006 Jan 27;311(5760):475-476.

• Peisajovich SG, Rockah L, Tawfik DS. (2006) "Evolution of new protein topologies through multistep gene rearrangements." Nat Genet. 2006 Feb;38(2):168-174.

• Khersonsky O, Tawfik DS. (2006) "The histidine 115-histidine 134 dyad mediates the lactonase activity of mammalian serum paraoxonases." J Biol Chem. 2006 Mar 17;281(11):7649-7656.

• Rosenblat M, Gaidukov L, Khersonsky O, Vaya J, Oren R, Tawfik DS, Aviram M. (2006) "The catalytic histidine dyad of high density lipoprotein-associated serum paraoxonase-1 (PON1) is essential for PON1-mediated inhibition of low density lipoprotein oxidation and stimulation of macrophage cholesterol efflux." J Biol Chem.2006 Mar 17;281(11):7657-7665.

• Aharoni A, Amitai G, Bernath K, Magdassi S, Tawfik DS. "High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments." (2005) Chem Biol., 2005 Dec;12(12):1281-1289.

• Khersonsky O, Tawfik DS. "Chromogenic and fluorogenic assays for the lactonase activity of serum paraoxonases." (2006) Chembiochem., 2006 Jan;7(1):49-53.

• Roodveldt C, Tawfik DS. "Shared promiscuous activities and evolutionary features in various members of the amidohydrolase superfamily." (2005) Biochemistry, 2005 Sep 27;44(38):12728-12736.

• James LC, Tawfik DS. "Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition." (2005) Proc Natl Acad Sci U S A., 2005 Sep 6;102(36):12730-12735.

• Gaidukov L, Tawfik DS. "High affinity, stability, and lactonase activity of serum paraoxonase PON1 anchored on HDL with ApoA-I." (2005) Biochemistry, 2005 Sep 6;44(35):11843-11854.

• Roodveldt C, Aharoni A, Tawfik DS. (2005) "Directed evolution of proteins for heterologous expression and stability." Curr Opin Struct Biol., 2005 Feb;15(1):50-56.

• Roodveldt C, Tawfik DS. (2005) "Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression in Escherichia coli results in stabilization of the metal-free state." Protein Eng Des Sel., 2005 Jan;18(1):51-58.

• Khersonsky O, Tawfik DS. (2005) "Structure-reactivity studies of serum paraoxonase PON1 suggest that its native activity is lactonase." Biochemistry, 2005 Apr 26;44(16):6371-6382.

• Aharoni A, Griffiths AD, Tawfik DS. (2005) "High-throughput screens and selections of enzyme-encoding genes." Curr Opin Chem Biol., 2005 Apr 9;(2):210-216.

• Gould SM, Tawfik DS. (2005) "Directed evolution of the promiscuous esterase activity of carbonic anhydrase II." Biochemistry, 2005 Apr 12;44(14):5444-5452.

• Bernath, K., Magdassi, S. & Tawfik, D.S. (2005) "Directed evolution of immunity protein inhibitors of colicin DNA-nucleases by in vitro compartmentalization (IVC) and nanodroplet delivery." J. Mol. Biol., Feb 4;345(5):1015-1026..

• Aharoni, A., Gaidukov, L., Khersonsky, O., Gould, McQ. S., Roodveldt, C. & Tawfik, D.S. (2005) "The evolvability of promiscuous protein functions." Nature Genetics 37:73-76.

• Harel, M., Aharoni, A., Gaidukov, L., Brumshtein, B., Khersonsky, O., Meged, R., Dvir, H., Ravelli, R.B.G., McCarthy, A., Toker, L., Silman, I., Sussman, J.L. & Tawfik, D.S. (2004) "Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotc enzymes." Nature Struct. & Mol. Biol. 11:412-419.

• Aharoni, A. & Tawfik, D.S. (2004) "Directed evolution of recombinant serum paraoxonase (PON) variants." Discovery Medicine 4:120-124.

• Bernath, K., Hai, M., Mastrobattista, E., Griffiths,A.D., Magdassi, S. & Tawfik, D.S. (2004) "In vitro compartmentalization by double emulsions: Sorting and gene enrichment by fluorescent activated cell sorting (FACS)." Anal. Biochem. 325:151-157.

• Bernath, K., Magdassi, S, & Tawfik, D.S. (2004) "In vitro compartmentalization (IVC): A high-throughput screening technology using emulsions and FACS. Discovery Medicine 4: 49-53.

• Cohen, H.M., Tawfik, D.S. & Griffiths, A.D. (2004) "Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization." PEDS 17:3-11.

• Aharoni, A., Gaidukov, L., Yagur, S., Toker, L., Silman, I. & Tawfik, D.S. (2004) "Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization." Proc. Natl. Acad. Sci. USA., 101: 482-487.

• James, L.C & Tawfik, D.S. (2003) "The specificity of cross-reactivity: Promiscuous antibody binding involves specific hydrogen-bonds rather than non-specific hydrophobic stickiness." Protein Sci. 12:2183-2193.

• James, L.C & Tawfik, D.S. (2003) "Conformational diversity and protein evolution – a 60 year old hypothesis revisited." Trends Biochem. Sci. (TiBS) 28:361-368.

• James, L.C., Roversi, P. & Tawfik, D.S. (2003) "Antibody multi-specificity mediated by conformational diversity." Science 299:1362-1367.

• Griffiths, A.D. & Tawfik, D.S. (2003) "Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalisation." EMBO J. 22:24-35.

• Sepp, A., Tawfik, D.S. & Griffiths, A.D. (2002) "Microbead display by in vitro compartmentalisation: Selection for binding using flow cytometry." FEBS Lett 532:455-458.

• Lee, Y-F., Tawfik, D.S. & Griffiths, A.D. (2002) "Investigating the target recognition of DNA cytosine-5-methyltransferase HhaI by library selection using in vitro compartmentalisation (IVC)." Nucleic Acid Res. 30:4937-4944.

• Cohen, H.M., Tawfik, D.S. & Griffiths, A.D. (2002) "Promiscuous methylation of many non-canonical sites by HaeIII methyltransferase." Nucleic Acid Res. 30:3880-3885.

• Lindner, A.B., Kim, S.-H., Schindler, D., Eshhar, Z. & Tawfik, D.S. (2002) "Esterolytic antibodies as mechanistic and structural models of hydrolases – a quantitative analysis." J. Mol. Biol. 320:559-572.

• James, L.C. & Tawfik, D.S. (2001) "Catalytic and binding poly-reactivities shared by two unrelated proteins - the potential role of promiscuity in enzyme evolution." Protein Sci. 10: 2600-2607.

• Hollfelder, F., Kirby, A.J. & Tawfik, D.S. (2001) "On the magnitude and specificity of medium effects in enzyme-like catalysts." J. Org. Chem. 66:5866-5874.

• Griffiths, A.D. & Tawfik, D.S. (2000) "Man-made enzymes – from design to in vitro compartmentalisation." Curr Opin Biotech. 11:338-353.

• Lindner, A.B., Eshhar, Z. & Tawfik, D.S. (1999) "Conformational changes affect binding and catalysis by ester hydrolysing antibodies." J. Mol. Biol. 285:421-430.

• Tawfik, D.S. & Griffiths, A.D. (1998) "Man-made cell-like compartments for molecular evolution." Nature Biotechnology 16:652-656.

• Hollfelder, F., Kirby, A.J. & Tawfik, D.S. (1996) "Off-the-shelf proteins that rival tailor-made antibodies as catalysts." Nature 383:60-63.