Bacteriology safety

In case of Emergency call the following number 08-934-2999

The following guidelines are general and do NOT replace the specific safety guidelines described in the instructions manual of instruments, and on the WIS safety unit web site (see links bellow):

General Guidfelines

  • All personnel should use basic laboratory protective gear. Where needed additional protective equipment should be used (see bellow).
  • In all cases a person working in the laboratory should wear clothes at all times. This should include long trousers, closed shoes, and a lab-coat.If an additional protection is needed you should follow the specific equipment’s instruction.
  • Never operate an instrument that you did not use in the past without the proper training.
  • When operating laboratory equipment, follow the safety signs (gloves, goggles, earphones), as well as the instruction card that is placed near the equipment.
  • Do NOT use an instrument if you suspect is not working properly. Notify us immediately!
  • If an instrument gets stuck, malfunctions, or breaks down, turn it off (where possible), apply a sign that describes the problem, and contact one of the personnel of the Bacteriology unit. Please don't leave the scene without informing us. The same holds when your materials get spilled/broken in any of the lab instruments (e.g. - shakers and sonicator).
  • For your and other people's safety always book/sign for the equipment that you are planning to use (Name, phone #, date, time, temperature: e.g. - shaker, sonicator).
  • The Bacteriology is considered a non radioactive, BL1 laboratory - it is therefore prohibited to bring pathogens and/or radioactive materials into the unit.
    For a list of organisms please refer to the NIH guidelines
  • It is prohibited to eat and drink in the laboratory: you should use either the kitchen or the office for that.
  • Wash your hands when you leave the lab, go to eat or drink, or have finished your daily work.

Chemicals and biological materials

  • When weighing harmful salts and chemicals use caution and the appropriate and/or additional protective gear (as described in their MSDS: e.g. - : nitrile gloves, goggles, and a mask as needed).
  • It is prohibited to throw into the normal trash liquid waste such as acids, bases, and flammables. Please dispose the different materials according to the WIS regulations (please refer to the WIS Chemical Safety web site .
  • The bacteriology is classified as BL1: It is therefore prohibited to use and grow pathogenic microorganisms, mammalian cells or tissues that are classified BL2 or more in the unit (see NIH guidelines)
  • It is prohibited to express infectivity factors including but not restricted to toxins, and virulence factors (see NIH guidelines)
  • Solid biological waste such as agar plates with bacteria, and plates with antibiotics should be collected into a biohazard bag, and subjected to autoclaving before being disposed as normal waste.
  • Liquid biological waste (media) should be decontaminated using bleach (1:10), Sodium-hypochlorite (1:10), or 0.1N NaOH for half an hour before disposing in the sink.

Spills

Minor spill

  • In case of minor spills of chemicals or biological materials try, if you can, to contain the spill.
  • If possible seal the affected area to prevent people from spreading the spill: do not leave the area before the area is marked correctly, sealed and the spill is under control.
  • If somebody got injured, or in case you have any problem, contact the safety personnel by dialing x2999, and don't forget to report the departmental office.
  • In case of an eye injury it is essential to wash the eyes immediately using an eye wash bottle, or using one of the eye jets found in the in the labs and the corridors.
  • If the spill reached the clothes, or the skin one needs to go and get an immediate and thorough shower, and change clothes.
  • If possible either decontaminate or adsorb the spill using neutral material (e.g. - using vermiculite). In case the spill is a biological material, collect the spill into a biohazard bag that should be destroyed by autoclaving.
  • Finally, for non-corrosive surfaces decontaminate the affected area using 1:10 diluted hypochlorite solution (when possible), followed by fresh water. Where not possible one should use 70% ethanol for decontamination, and further consult the WIS Safety Unit.
  • In case you are not sure how to clean or decontaminate the affected area call and consult with the safety unit personnel.

Major Spill

  • In case of a major spill isolate the area, prevent people from entering or leaving the area, and call immediately the Safety Unit at x2999.
  • In case of injury call immediately the Safety Unit at x2999, and start treating the wounded person until a paramedic arrives.
  • In case of an eye injury it is essential to wash the eyes immediately for at least 10 minutes using an eye wash bottle, or using one of the eye jets found in the in the labs and the corridors.
  • If the spill reached the clothes, or the skin one needs to go and get an immediate and thorough shower.
  • Only after completing the above, one should engage in the decontamination process.
  • To decontaminate or adsorb the spill use neutral material (e.g. - vermiculite). In case the spill is a biological material, collect the spill into a biohazard bag that should be destroyed by autoclaving.
  • Finally, for non-corrosive surfaces decontaminate the affected area using 1:10 diluted hypochlorite solution (if possible), followed by fresh water. Where not possible one should use 70% ethanol for decontamination, and further consult the WIS Safety Unit.
  • In case you are not sure how to clean or decontaminate the affected area call and consult with the Safety Unit personnel.

Autoclave SIP fermentor and hot media

  • When loading and unloading or operating the autoclave, and when working with a hot fermentor, one should use heat resistant gloves to prevent burn injuries.
  • To avoid breaking/spillage of media from bottles and flasks always use a cart to move and carry materials.

Fire

  • Always turn off the bunsen when not in use. Minimize moving around with a working bunsen.
  • It is prohibited to use fire in the kitchen.
  • In order to avoid burns and sticking of synthetic materials made of plastic or rubber, avoid using them when working with open flame (e.g. – gloves, lab coats).

Sonicator and continuous centrifuges

  • Always use earphones when operating the sonicator or the continuous centrifuges to protect your ears (it is even better combining earphones on top).
  • You must operate both instruments with goggles to protect your eyes.

Microfluidizer and SIP fermentor

  • You must operate both instruments with goggles to protect your eyes.
  • Wear gloves when using the microfluidizer.
  • To prevent spill of pressurized biological material it is prohibited to open screws or lids of the fermentor or microfluidizer while being operated, and while under pressurized conditions.