DNA sequencing about

The DNA Sequencing unit provides automated dideoxy sequencing based on dideoxy nucleotides that have been chemically modified to fluoresce under UV light (four different fluorescent colors for the four dideoxy nucleotides A, G, C, T).

Automated DNA sequencing is done using a 3730 DNA Analyzer from ABI, based on capillary electrophoresis. All users receive a colored densitometric scan which displays four different colored peaks corresponding to the different bases. Double-stranded DNA plasmids, BACs, single-stranded DNA, and PCR fragments are suitable for sequencing.