Flow cytometry resources

Available Resources

  1. LSRII-new cell analyzer with five lasers :

    1. Coherent Sapphire Solid state 488nm, 20mW laser with:

      1. 685LP, 710/50BP (PerCP-Cy5.5)
      2. 505LP, 525/50BP (Alexa488, FITC, GFP, CFSE, YFP)
      3. None, 488/10BP (SSC)
    2. Coherent COMPass YellowGreen 561nm, 25mW with:

      1. 740LP, 780/60BP (PE-Cy7, PE-Alexa 750)
      2. 685LP, 710/50BP (PE-Alexa 680, PE_Cy5.5)
      3. 635LP, 660/20BP (PE-Cy5, PE-Alexa 647, 7AAD)
      4. 600LP, 610/20BP (PI, Alexa 594, HCred, mCherry,mRFP1, PE-Texas Red, Alexa 568, PE- CF594)
      5. 570LP, 582/15BP (DsRed, PE, DsRed2, dTomato,Cy3)
         
    3. JOS Uniphase 1344p HeNe Red 633nm, 40mW with :

      1. 740LP, 780/60BP (APC-Cy7, APC-Alexa 750,APC-H7)
      2. 685LP, 710/50BP (Alexa 680, APC-Cy5.5, Alexa 700)
      3. None, 670/30BP (APC, Alexa 647, Cy5)
    4. Coherent Vioflame solid state Violet 407nm, 50mW with:

      1. 740LP, 780/60BP (Qdot 800)
      2. 685LP, 705/70BP (Qdot 705)
      3. 565LP, 610/20BP (Qdot 605, Brilliant Violet 605)
      4. 505LP, 525/50BP (Alexa 430, CFP, Qdot 565,AmCyan, Horizen V500)
      5. None, 450/50BP (Alexa 405, Cascade-Blue, PacificBlue, eFluor 450, Horizen V450, Brilliant Violet 421, CFP)
    5. lightwaveXcyte solid state UV 355nm, 60mW laser with:

      1. Includes the option of BD High Throughput Sampler (HTS).
      2. 505LP, 530/30BP (Hoechst Red, Indo1 blue)
      3. none, 450/50BP (Alexa 350, Hoechst, Indo1 violet,DAPI
         
  2. LSRII cell analyzer with three lasers:

    1. Coherent Sapphire Solid state 488nm, 20mW with:

      1. 755LP, 780/60BP (PE-Cy7)

      2. 685LP, 695/40BP (PerCP-Cy5.5, PerCP)

      3. 655LP, 670/14BP (PE-Cy5, Nile red, 7AAD)

      4. 600LP 610/20BP (PE-Texas Red, Propidiume Iodide, Diyhdroethidium, PE- CF594)

      5. 550LP 575/26BP (PE, PI, DsRed, Dil)

      6. 505LP 530/30BP (FITC, Alexa 488, GFP, SYBR green, YFP,CFSE) 

      7. None 488/10BP (SSC)

    2. JDS Uniphase HeNe 633nm, 17mW with:

      1. 755LP, 780/60BP (APC-Cy7, APC-H7, Alexa 750)

      2. 710LP, 730/45BP (Alexa 700)

      3. None, 660/20BP (APC, Cy5, DiD)

    3. Point Source Violet Solid State 407nm, 25mW with:

      1. 505LP, 525/50BP (Alexa 430, AmCyan, Horizen V500, CFP)

      2. None 450/50BP (Pacific Blue, Alexa 405, Marine Blue, CFP, coumarine Hcc2, eFluor450, Horizen V450, Brilliant Violet 421)

  3. FACSAria-FUSION with five lasers. The blue, yellow-green and red can be used either with the violet or the nUV:

    1. 488nm – Blue – 50mW (Coherent Sapphier)

      1. 655 LP, 695/40 BP - 695/40-B, PerCp-cy5.5 ,PerCP

      2. 502 LP, 530/30 BP - 530/30-B, FITC, Alexa 488, Brilliant Blue 515 , GFP, CFSE, YFP, SYBR Green

    2. 561nm - Yellow Green – 50 – mW (Coherent Sappier)

      1. 735,780/60 BP - 780/60-YG, PE-Cy7, PE-Alexa 750

      2. 736, 735 685 LP, 710/50 BP - 710/50-YG, PE-Cy5.5, PE-Alexa 680

      3. 630 LP, 670/14 BP - 670/14-YG, PE-Cy5, 7AAD, PE-Alexa 647

      4. 600 LP, 610/20 BP - 610/20-YG, PI, PE- CF594, PE-TxRed, Alexa 594, mCherry, mRFP1,Alexa 568

      5. None, 582/15 BP – 582/15-YG, PE, DsRed, dTomato, Cy3

    3. 640 nm –Red – 100mW (Coherent Obis)

      1. 755 LP, 780/60 BP - 780/60-R, APC-Cy7, APC-H7, APC-Alexa 750

      2. 690 LP, 730/45 BP - 730/45-R, Alexa 700

      3. None, 670/30 BP - 670/30-R, APC, Alexa 647, Cy5

    4. 405nm –Violet – 85mW (Point Source Iflex 2000)

      1. 750LP, 780/60 BP – 780/60-V, BV786, Qdot 800

      2. 690LP, 710/50 BP - 710/50-V, BV711, Qdot 705

      3. 630LP, 660/20 BP - 660/20-V, BV660

      4. 595LP, 610/20 BP - 610/20-V, BV605, Qdot 605

      5. 505LP, 525/50 BP - 525/50-V, AmCyan, Horizon V500, BV510, Alexa430

      6. None, 450/50 BP - 450/50-V, Pacific Blue, Horizon V450, BV421, Alexa 405, Cascade-Blue, eFluor 450, CFP, DAPI-Violet

    5. 375nm-nUV-7mW (Fabrinet)

      1. 610 LP, 670 LP - 670LP-nUV, Hoechst Red

      2. None, 450/20 BP – 450/20-nUV, DAPI-nUV, Hoechst Blue

  4. FACSAriaIII cell sorter with four lasers: 

    1. Coherent Blue 488nm 20mW with:

      1. 655 LP 695/40 BP (PerCp-cy5.5),PerCP)

      2. 502 LP 530/30 BP (FITC, CFSE, GFP, YFP, Alexa 488)

      3. None 488/10 BP (SSC)

    2. Coherent Yellow Green 561nm 40mW with:

      1. 735 LP 780/60 BP (PE-Cy7, PE-Alexa 750)

      2. 685 LP 710/50 BP (PE-Cy5.5, PE-Alexa 680)

      3. 630 LP 670/14 BP (PE-Cy5, PE-Alexa 647, 7AAD)

      4. 600 LP 610/20 BP (PI, PE-TxRed, Alexa 594, mCherry, HCred, mRFP1, PE- CF594)

      5. None 582/15 BP (PE, DsRed, Alexa 568, dTomato, DsRed2)

    3. JDSU HeNeRed 633 nm 20mW with:

      1. 755 LP 780/60 BP (APC-cy7, APC-Alexa 750) APC-H7

      2. 690 LP 730/45 BP (APC-Cy5.5, Alexa 700)

      3. None 660/20 BP (APC, Cy5, Alexa 647)

    4. Coherent Violet 407nm 50mW with:

      1. 735 LP 780/60 BP (QDOT 800)

      2. 690LP  710/50 BP (BV711, Qdot 705, eFluor 700NC,

      3. 630LP  660/20 BP (BV650, Qdot 655, eFluor 650NC, eVolve 650)

      4. 595LP 610/20 BP  (BV605, Qdot 605, eFluor 605NC, eVolve 605)

      5. 502LP 510/50 BP (Alexa 430, CFP, QD 525, AmCyan, Horizen V500, CFP)

      6. None 450/40 BP (Alexa 405, Pacific Blue, Cascade Blue, eFluor 450, Horizen V450, BV421, CFP, DAPI-violet)

  5. SORP FACSAriaII cell sorter with five lasers:

    1. UV Genesis 355nm 65mW with:

      1. 505LP 530/30 BP (Hoechst red, Indo-1 blue)

      2. None 450/50 BP (Alexa 350, Hoechst, Indo-1 (Violet), DAPI-UV)

    2. Violet CUBE 405nm 100mW with:

      1. 750LP 780/60 BP (Qdot 800)

      2. 670LP 705/70 BP (Qdot 705)

      3. 595LP 605/40 BP (Qdot 605, Brilliant Violet 605)

      4. 505LP 525/50 BP (Alexa 430, AmCyan, CFP, Qdot 525, Horizen V500, CFP)

      5. None 450/50 (Alexa 405, Cascade Blue, Pacific Blue, DAPI- Violet, CFP,Horizen V450, Brilliant Violet 421)

    3. Violet CUBE 405nm 100mW with:

      1. 750LP 780/60 BP (Qdot 800)

      2. 670LP 705/70 BP (Qdot 705)

      3. 595LP 605/40 BP (Qdot 605, Brilliant Violet 605)

      4. 505LP 525/50 BP (Alexa 430, AmCyan, CFP, Qdot 525, Horizen V500, CFP)

      5. None 450/50 (Alexa 405, Cascade Blue, Pacific Blue, DAPI- Violet, CFP,Horizen V450, Brilliant Violet 421)

    4. Blue Sapphire 488nm 100mW with:

      1. 685LP 710/50 BP (PerCp-Cy5.5)

      2. 505LP 525/50 BP (Alexa 488, FITC, GFP, CFSE, YFP)

      3. None 488/10 BP (SSC)

    5. YellowGreen Compass 561nm 110mW with:

      1. 750LP 780/60 BP (Pe-Cy7, PE-Alexa 750)

      2. 685LP 710/50 BP (PE-Alexa 680, PE-Cy5.5)

      3. 635LP 660/20 BP (PE-Cy5, PE-Alexa 647)

      4. 600LP 610/20 BP (PE-Texas Red, PI, Alexa 594, HCred,mCherry, mRFP1, PE- CF594)

      5. None 582/15 BP (DsRed, PE, DsRed2-Alexa 568, dTomato)

    6. Red CUBE 640nm 40mW with: 
      Four-way sorting and analysis of up to 16 parameters using FACSDiva software.

      1. 750LP 780 BP (APC-Cy7, APC-Alexa 750, APC-H7)

      2. 685LP 710/50 BP (Alexa 680, Alexa 700, APC-Cy5.5)

      3. None 670/14 BP (APC, Alexa 647, Cy5)

  6. FACSAriaII cell sorter with three lasers:

    1. Coherent Sapphire Solid state 488nm, 20mW with:

      1. 735LP 780/60 BP (PE-Cy7, aposense)

      2. 655LP 695/40 BP (PE-Cy5, PerCp, PerCp-Cy5.5, PI, 7AAD, Cy- Chrome)

      3. 595LP 610/20 BP (PE-Texas Red, HcRed1, Propidium Iodide, PE- CF594

      4. 550LP, 575BP (DsRed, PE, Cy3, DsRed2, RFP)

      5. 502LP 530/30 BP (Alexa 488, FITC, GFP, CFSE, SYBR Green, YFP)

      6. None 488/10 BP (SSC)

    2. JDS Uniphase HeNe 633nm, 17mW with:

      1. 735LP 780/60 BP (APC-Cy7, APC-H7, APC-Alexa 750)

      2. None 660/20 BP (APC, Alexa 647, Cy5, Did)

    3. Point Source Violet Solid State 407nm, 25mW with:

      1. 502LP 530/30 BP (Alexa 430, AmCyan.,CFP,Horizen V500)

      2. None 450/40BP (CFP, DAPI, Hoechst Blue, Marina Blue, Pacific Blue, Horizen V450, Brilliant Violet 421)

  7. ImageStreamX Flow Imager system with 5 lasers:

    1. Excitation lasers:

      1. Violet 405 nm, 175mW

      2. Blue 488 nm 100 mW

      3. Yellow-Green 561 nm, 200 mW

      4. Red 642 nm 150 mW

      5. Darkfield laser 785 nm (SSC).

    2. Emission channels (nm):

      1. 430-480: Brightfield

      2. 480-560: FITC, GFP, AF488, YFP

      3. 560-595: PE, Cy3, AF546, AF555

      4. 595-660: RFP, PI, mCherry, AF568

      5. 660-745: PerCP, PE-Cy5, PE-AF647

      6. 745-800: PE-Cy7, PE-AF750, SSC

      7. 430-505: DAPI, Hoechst, Pacific blue, CFP, A405

      8. 505-570: PacOrange, AF430, QD525

      9. 570-595: Brightfield

      10. 595-640: QD625, eFluor625

      11. 640-745: APC, Cy5, AF647, Draq5, PerCP

      12. 745-800: QD800, APC-Cy7,APC -AF750,Cy7, AF750

    3. The ImageSreamX contains:

      1. Two cameras, 12 Imaging Channels

      2. 20X,40X and 60X magnification

      3. Extended Depth of Field optics

      4. Single color brightfield

      5. Autosampler for 96 well plates

      6. Data Analysis Workstation with IDEAS software.

  8. ImageStream-NEW Flow Imager system with 5 lasers

    1. Excitation lasers:

      1. Violet 405 nm 120mW

      2. Blue 488nm 400 mW

      3. Yelow-Green 561nm 200 mW

      4. Red 642 nm 150 mW

      5. Darkfield laser 785 nm (SSC)

    2. Emission channels (nm):

      1. 430-480: Brightfield

      2. 480-560: FITC, GFPm AF488, YFP

      3. 595-660: RFP, PI, mCherry, AF568

      4. 660-745: PerCP, PE-Cy5, PE-AF647

      5. 745-800: PE-Cy7, PE-AF750, SSC

      6. 430-505: DAPI, Hoechst, Pacific blue, CFP, A405

      7. 505-570: PacOrange, AF430, QD525

      8. 570-595: Brightfield

      9. 595-640: QD625, eFluor625

      10. 640-745: APC, Cy5, AF647, Draq5, PerCP

      11. 745-800: QD800, APC-Cy7, APC-AF750, Cy7,AF750

    3. The ImageStreamX contains:

      1. Two cameras, 12 Imaging Channels

      2. 20X, 40X and 60X magnification

      3. Single color brightfield

      4. Data Analysis Workstation with IDEAS software

  9. CyTOF2 Mass Cytometer upgraded to Helios

  10. EVOS FL Auto 2 Imaging System

    1. Fully-automated inverted multi-channel fluorescence and transmitted light imaging system.

    2. Equipped with on stage incubator with CO2 and temperature control.

    3. Available lenses: 4X, 20X.

    4. Available channels - brightfield and green (GFP, FITC, AF488)

  11. Additional Equipment

    • A PC with FlowJo, FCS-express and Modfit for off line analysis.
    • An_Heraeus_Labofuge 400 Centrifuge.
    • An HERA cell Heraeus_Incubator.
    • A NUAIR Biological Safety Cabinet.
    • Floor grounding to avoid static electricity.
    • Individual air conditioning with positive pressure. Temperature maintained between 22-24C.
    • Central air pressure outlet allowing up to 80 PSI.
    • Vacuum pump.
    • Fluorescent room lighting and additional rheostat control.
    • Walls and sinks with non-reflective surfaces.
    • Safety:
      A danger sign at the entry to the laboratory.
      A red light to be turned on when lasers are aligned.
      A main disconnect for the lasers located near the operator.
      Electric locking of the room with magnetic cards to enter