Whats New

2016
05
Oct

DasBox small scale multi fermentation system (75-200ml).

The Genomic Repository Unit is happy to announce the introduction of a second DasBox small scale multi fermentation system (75-200ml). This unit is very similar to the already installed system, with an extra feature: Off Gas analyzer.

The Off Gas analyzer permits to measure the levels of CO2 and O2 in the exhaust of the bioreactors, which can be used to calculate the rates of oxygen and carbon dioxide consumption/production, and from it to calculate the net growth rates.

This system, as the previous unit, is suitable for a many applications, among which the most common are:

  • Replicate fermentation experiments.
  • Develop upstream processes (USP: using Batch & Fed-Batch fermentations).
  • Long term fermentations using chemostat or turbidostat modes (e.g. – for evolution of cell lines).
  • Long/short term competition experiments.

For more information feel free to contact Dr. Ghil Jona at 08-9346026, and Dikla Levi at 08-9346027

 

2015
04
Feb

Blue Pippin

The platform features the ability for targeted size selection of DNA fragments (90bp-50kb) with minimal effort for the purpose of Next-Generation Sequencing. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination. The Blue Pippin can also use SDS-agarose gel cassettes to collect targeted protein sizes from complex samples for biomarker or protein modification studies.

For more information feel free to contact Dr. Dudu Pilzer or Muriel Chemla at 08-9344435

2014
05
Nov

DasBox

We are happy to announce the introduction of DasBox, a small scale multi fermentation system (75-200ml), that permits to grow in parallel up to 4 cultures of microorganisms (e.g. - bacteria & yeast). The system is very sophisticated and enables to measure and regulate over a dozen distinct variables, using an array of either simple or complex conditional functions that can be induced manually or based on the output values from the different measured variables.

This system is suitable for a many applications, among which the most common are:

  • Replicate fermentation experiments.
  • Develop upstream processes (USP: using Batch & Fed-Batch fermentations).
  • Long term fermentations using chemostat or turbidostat modes (e.g. – for evolution of cell lines).
  • Long/short term competition experiments.

For more information feel free to contact Dr. Ghil Jona at 08-9346026

2014
28
Oct

Irradiation news

The Biological Services unit strives to offer its clients the most innovative, progressive services.

In order to keep up with the changes in the irradiation field, early August 2014 the biological services unit has purchased a state of the art X-Ray biological irradiator (suitable  both for small animal and specimen irradiation) , the XRAD 320 by Precision X-Ray (PXI).

The facility enables a wide range of experimental investigations, particularly in the field of biology and plant genetics. Almost anything can be irradiated, from all types of test tubes, cells  and seeds and plants etc. The unit is located in the X-Ray building.

2014
16
Jul

FPLC machine

The antibody unit is going to purchase a new FPLC machine. The AKTA start, which is use for protein purification: tagged proteins and antibodies. We would like to offer a new service: Antibody purification on protein A or G.
If you have medium of monoclonal antibody or polyclonal antibodies in serum, we can purified the antibody either on protein A or G column.

For more details feel free to contact Dr. Orith Leitner at 08-9342139.

 
2014
18
Jun

CyTOF™ Mass Cytometer

The CyTOF™ Mass Cytometer analyzes single cells, labeled with stable heavy metal isotopes (instead of fluorescent stains), using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The instrument offers the specificity, dynamic range and quantitative capability of mass spectrometry in a format that is familiar to Flow Cytometry users. Thus, the CyTOF™ combines the advantages of single cell high speed analysis common to Flow Cytometry with the ability to resolve over 100 metal probes with minimal signal overlap common to Atomic Mass Spectroscopy, thereby providing researchers with an unparalleled ability to phenotypically and functionally profile cells from normal and diseased states.

For more information feel free to contact Dr. Ayala Sharp or Eitan Ariel at 08-934-2721 / 2235

 
2014
18
Jun

Fluidigm C1™

We are happy to announce purchase of the Fluidigm C1™ Single-Cell AutoPrep System to the Microarrays unit. C1 performs single cell isolation, allows prompt and reliable individual cells isolation and process for genomic analysis. It can isolate cells, extract RNA, and then reverse transcribe and preamplify mRNA transcripts automatically to detect and analyze cell activity. http://www.Fluidigm.com/C1system.htm

For more information feel free to contact Dr. Dudu Pilzer or Muriel Chemla at 08-9344435

   
2013
29
Dec

Karyotyping Service

The recent SCC&TCCU addition is Complete Multicolor Karyotyping & FISH System (HiSKY) from ASI. Karyotyping is the process of pairing and ordering all the chromosomes of an organism, thus providing a genome-wide snapshot of an individual's chromosomes.
ASI HiSKY system enables robust color karyotype analysis of normal and genetically modified embryonic stem cells (ESCs) as well as induced pluripotent stem cells (iPSCs). It allows study of chromosomes from different species: human, mouse, rat, cattle and many others. It is also especially advantageous in various cancer-related studies for diagnostics and evaluation of cellular genomic integrity.

For more information feel free to contact Dr. Elena Ainbinder at 08-9346539

 
2013
29
Dec

Large Scale Cell Expansion Service

The latest SCC&TCCU acquisition is GE Healthcare Life Sciences' ReadyToProcess WAVE 25 bioreactor system. The WAVE 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 L. The system is based on a well known rocking technology that provides mixing and aeration to the culture, which is maintained in an inflated, disposable Cellbag™ bioreactor cultivation chamber. The ReadyToProcess WAVE 25 is the newest modification of Wave bioreactor system where the rocking technology is enhanced with features such as advanced sensors and intelligent control strategies. System operation is easily managed via the intuitive and user-friendly UNICORN™ system control software. The system is designed for fast installation and convenient handling, and delivers a reliable and accurate performance suitable for research aims.

For more information feel free to contact Dr. Elena Ainbinder at 08-9346539

 
2013
22
Dec

BioFlo115

We are happy to announce the latest addition in the Bacteriology unit – BioFlo115 fermentor. We have recently introduced to the Bacteriology unit a new small scale fermentor, the BioFlo115. This fermentor along with its three siblings enables us to run fully controlled, fermentations between 0.5 and 100 liters (0.5-2 lit mini runs, through 5-10.5 lit runs, and 40-100 lit pilot runs).
We also supply process development to find the best conditions for growing and expressing your protein of interest in bacteria and yeast, whether in standard culture media, or in defined and/or specialized that can support the growth of high cell density cultures (up to optical densities of 30-70ODs).

For more details on High Density fermentations, and Upstream Process Development, please contact
Dr. Ghil Jona, at 08-9346026

 
2013
22
Dec

ORFeome collection

The Genomic Repository unit is happy to announce that we have recently received the human ORFeome collection version 5.1. This collection comprises of over 16,000 human ORF clones in a Gateway Entry vector. The ORFs can be either transferred to one of the various human or bacterial Gateway expression vectors, or amplified using PCR for conventional or RF cloning. On the plasmid bank web site you can search for vectors and different E. coli bacterial strains from the large stock.

 
2013
14
Dec

Shimadzu PPSQ- 31A protein sequencer

We are happy to announce the addition of the Shimadzu PPSQ- 31A protein sequencer to the Biological Mass Spectrometry Laboratory.Using automated Edman Degradation Sequencing, also known as Gas Phase Sequencing, we can work on samples in solution or from proteins that have been separated using SDS-PAGE and blotted onto PVDF membranes. N-terminal sequencing can also be used for de novo sequencing of proteins.

The sequencer will:

  • Sequentially break down the peptide or protein into its constituent amino acids from the N-terminus side of the sample
  • Derivatize and separate the produced amino acids by reverse-phase high pressure liquid chromatography
  • Visualize the amino acids by UV detection for each cycle of Edman chemistry

For more information feel free to contact Tevi Mehlman at 08-9344451