INSTRUMENTATION

Autoclave

Tuttnauer 3870 EL autoclave is used in order to prevent contamination of viruses, bacteria or fungi on equipment or in liquids (media, buffer…) can be prepared ether by filtration, trough a 0.22 μm filter, or by using the autoclave. Sterilizing by autoclave is done with steam at 121oC in a pressure chamber, for approximately 20 minutes. 

Shaker incubators

Several shaker incubators allow growth of bacteria at different temperatures, leading to different growth rates. Biological materials (proteins, DNA...) are usually produced in bacteria. Control of growth rate may be very important in the production of certain proteins. 

Sonicator

A Sonics Vibra-Cell VC 750 sonicator uses ultrasonic vibrations for disruption of cell membranes. The unit has several different probes, for diverse sample volume and sonication amplitudes: Stepped microtip, Tapered microtip, 13mm and Cup Horns. Cell lysis is a first step in purifying specific contents produced in cells. 

Centrifuges

A variety of centrifuges is available in the unit, for different centrifugation speed and sample volume. 

Polymerase Chain Reaction (PCR) instruments

The unit has an Eppendorf Mastercycler Gradient and an Eppendorf Mastercycler Personal PCR apparatuses. PCR amplifies exact DNA sequences. It can also be used to create specific changes in DNA sequences (site directed mutagenesis) and insert DNA sequences into specific sites (restriction free cloning). A Real Time or quantitative PCR instrument (Applied Biosystems StepOnePlus) allows quantification of DNA template in the PCR mixture. Differential RNA/DNA expression is done by following in real time DNA amplification. 

Fast protein liquid chromatography (FPLC)

The AKTA Avant 25 is the latest-generation FPLC. This system’s automated and easy-to-use protein scanning capabilities enables us to create protein-purification methods or perfect existing ones. The AKTA Avant 25 and AKTA Basic systems are used for protein purification. Required proteins can be separated from the cell lysate by FPLC. Using columns that separate proteins according to their size, charge, hydrophobicity… a protein can be purified from the protein mixture. The unit holds a wide variety of chromatographic columns.

Gel Electrophoresis Devices

Nucleic acids and proteins can be separated according to their size using gel electrophoresis. This assay shows whether the sample contains the desired product and how pure it is. Gel electrophoresis can also be used for purifying specific nucleic acids.

Spectrophotometers

Assay transmittance (or reflectance) of light through a sample at specified wavelengths. The unit has a Cary 300 Bio spectrophotometer with different accessories. The unit’s NanoDrop is a spectrophotometer that can measure transmittance of a very small volume (1.5 μl).

Microplate Reader

The BioTek Synergy HT is a Multi-Mode Microplate reader. It measures transmittance, fluorescence and luminescence in microplate (96-well, 384-well…) format. The plate reader is equipped with two injectors, allowing automated injection of material to start the reaction, thus increasing accuracy of measurements especially in kinetic mode. 

Fluorescence Laser Scanner

Typhoon FLA 9500 Biomolecular Imager is a versatile, state-of-the-art laser scanner suited for applications such as sensitive and quantitative measurements of multiplex fluorescence, Western blots, and radioisotopic labels as well as digitization of colorimetric stains. It scans at a resolution of up to 10 μm useing three lasers (473nm, 532nm, 635nm) and matching filters (>510nm, >575nm, >665nm, 520-540nm, 560-580nm).

Radioactive Workstation

The only lab in the Perlman building in which radioactive work (32P and 35S) is permitted is the NanoBio unit. Even though many procedures have switched from radioisotope labeling to fluorescence labeling, there are still instances in which radioisotope-labeled samples are the best option (especially when working on surfaces). 

Cell Disrupter

TS Series Cell Disrupter is used to disrupt the membrane of cells by passing them through a narrow valve under high pressure. By controlling the pressure it is possible to break different membranes in cells or different cell types. In this way you can separate different components of the cell (e.g. for E. coli you can differentiate between, Release of DNA, Cytoplasmic Protein, Inclusion Bodies, Membrane protein/material) or break bacterial, fungal, plant or animal cells.