About the service

The Chemical Analysis and Mass spectrometry Laboratory provides training, consultation, and method development for measurement, separation, and isolation of a wide range of organic compounds by Mass Spectrometry, elemental analysis, amino acid analysis, Raman, Micro Raman, Micro Infra-Red (IR) spectroscopy, CD spectroscopy and Thermo Gravimetric Analysis (TGA).

We provide molecular weight deterimination and elucidating the structure of organic compounds up to 4000 AMU including labile metal complexes, and for polymers, peptides and proteins up approx. 60,000 AMU. Detection limit approx. 50 pg.

The Mass Spectroscopy section is equipped with 4 different spectrometers:

  • Micromass ZQ mass spectrometer used primarily for routine identification and standard work with small organic molecules.
  • Field Desorption (FD) for identification and accurate mass determination of volatile and labile metal complexes.
  • Maldi for determining high mass polymers, peptides, DNA.
  • Inductively Coupled Plasma Mass Spectrometry (ICP MS) provides analysis for a variety of elements, and with a sensitivity of a few ppb (ug/lit), depending on the analyte.

Elemental Analyses determines the amount of C, H, and N in organic or inorganic compounds.

The Amino Acid Analyzer provides qualitative and quantitative analysis of protein and peptide hydrolyzates. Detection range of 100-3000 pmoles, using OPA and FMOC pre-column derivatization, monitoring at UV, using reverse phase separation. Detection range of 5-3000 pmole using AccQ. Tag pre-column derivatization and monitoring of fluorescent emission.

We provide Raman measurements and micro IR transmission and reflection measurements.

  • FT Raman using 1064 µm 1.5W Laser excitation.
  • Micro Raman provides micro Raman measurements, using 633 nm laser excitation.

The ThermoGravimetric Analysis (TGA) provides material characterization by monitoring a substance’s mass as a function of temperature or time as the sample specimen is subjected to a controlled temperature program in a controlled atmosphere.
 

The Zetasizer system provides measurement of size, molecular weight, mobility and improved sensitivity of zeta potential on dispersed particles and molecules in solution.
Malvern’s Zetasizer Nano ZSP uses DLS (Dynamic Light Scattering) to measure size of colloids, nanoparticles and molecules ranging 0.3nm-10microns,  zeta potential range of > +/-500mV and a Molecular weight range of <1000Da - 2 x 107Da.

Ilit Cohen Ofri
Ilit Cohen Ofri
Head
Tel. 08-934-6956