About the service

The X-ray Crystallography Laboratory of the Weizmann Institute is both a service and user facility. It is well equipped for the single crystal diffraction experiments needed for structural biology and chemistry research. We apply a variety of experimental methods to these investigations.

Small Molecules

As each crystal is unique, we plan each experiment strategy to the individual sample and offer expertise in the following areas:

  1. Air sensitive crystals
  2. Unusually small crystals
  3. Range of temperature data collection from 90-500K
  4. Disorder or twinning
  5. Absolute structure determinations, including all-light atom structures
  6. Crystal morphologies
  7. Database searches and analysis for similar structures or packing motifs

A structure determination of molecular crystals will typically involve the following:

  1. Crystal Sample inspection under the polarizing microscope
  2. Determination of unit cell parameters, crystal system and space group
  3. Structure solution and refinement
  4. Creation of tables in CIF and other formats
  5. Publication-ready molecular and packing plots

The measurements of organic and organometallic materials are performed using a Rigaku XtaLabPro diffractometer with both Cu and Mo sources and equipped with a Dectris Pilatus S200 detector.  Also available is a Bruker Kappa ApexII CCD diffractometer utilizing Mo radiation. Data collection is typically collected at 100K temperatures using an Oxford Cryostream systems. This yields higher quality data and helps to protect air sensitive crystals from decay. In cases when phase transitions may be taking place, or if required, measurements may be made between 90K-500K.

Macromolecular  Crystallography

For the Structural Biologists, the X-ray laboratory is a user facility. Macromolecular crystallographic measurements are made on a R-Axis IV++ systems. The image plate detectors are mounted on a MX007HF generator equipped with VariMax optics HF This system is outfitted with a 2-theta stage allowing high-resolution data collection. The  system is equipped with Oxford cryostream cooling systems for LN, low-temperature measurements. Also available in the laboratory are UV/Visible light-microscopes for sample inspection and mounting as well as a digital camera for crystal photography.

Training is available for users, so that they can perform their own experiments.

Linda  J. W. Shimon
Linda J. W. Shimon
Head
Tel. 08-934-6563, 050-832-5575