Chemical Services, NMR Unit High-Resolution NMR Facility

NMR Instrumentation at the High Resolution Facility

• AVANCE III-800 Bruker
• DMX-500 Bruker
• AVANCE-500 Bruker
• AVANCE III-400 Bruker
• AVANCE III-300 Bruker

In addition, the NMR Unit comprises an imaging laboratory equipped with a Bruker AV III-400 widebore spectrometer used for NMR microscopic imaging, a 4.7 Tesla horizontal bore Biospec system (Bruker, AVANCE III) as well as a 9.4 Tesla Biospec used mainly for imaging experiments.
Solid state NMR is done at the Department of Chemical Physics.

AVANCE III-800 Bruker

The pumped DRX-800 NMR spectrometer located at the Ruthie & Samy Cohn building, was installed on December 1998. The Bruker DRX-800 console system was recently upgraded to AVIII system. It provides access to the highest magnetic field currently available of commercial spectrometers in Israel, enabling state-of-the-art high-resolution experiments for macromolecular structure determination. This four-channel instrument is used mainly for specialized research, including 3D and 4D NMR and biologically oriented work. It has facilities for CIDNP experiments, variable temperature studies, pulse shaping, 'X' and 'Y'-nucleus continuous-wave or broadband decoupling. It can perform a wide range of modern gradient-selected experiments, including automatic deuterium gradient shimming, diffusion studies etc.
Its accessories include:
A new, 5mm Triple-Resonance Inverse CryoProbe, TCI, (1H, 13C, 15N) equipped with Z-gradients and with an Automatic Tuning and Matching (ATM), used mainly for protein studies.
A 5mm Triple-resonance Inverse TXI probe (1H, 13C, 15N) equipped with z gradient, and
A 5mm multi-nuclear QXI probe with x, y and z gradients (1H, 15N, 13C, 31P).

DMX-500 Bruker Avance

The 500 MHz spectrometer that has an Oxford magnet resides at the western Wolf building. The Bruker AMX-500 console system was upgraded during 1996 to the "Avance DMX" version. It is used mainly for specialized research, including 2D NMR and biologically oriented work. This three-channel instrument has facilities for CIDNP experiments, variable temperature studies, pulse shaping, 'X' and 'Y'-nucleus continuous-wave or broadband decoupling. It can perform a wide range of modern gradient-selected experiments, including automatic deuterium gradient shimming, diffusion studies etc. It is equipped with the following probeheads:

• A new, 5mm Triple-Resonance Inverse CryoProbe, TXI, (1H, 13C, 15N) equipped with Z-gradients, used mainly for protein studies (Bruker)
• A 5mm Triple-Resonance Inverse probe, TXI, (1H, 13C, 15N) equipped with Z-gradients, used mainly for protein studies (Nalorac)
• A selective 5 mm proton probe
• A 5mm dual probe (1H, 13C)
• A 10mm multinuclear, Broad Band (BB) probe (109Ag to 31P)
• A 10mm automatic QNP probe, switchable by computer for 1H, 15N, 31P and 13C nuclei
• A 4mm 13C CP-MAS probe.

AV-500 Bruker Avance

The UltraShield Bruker AV-500 spectrometer was installed at the Sieff building (Organic Chemistry department) in 2003. It mainly serves scientists in the Department of Organic Chemistry and provides a modern facility for their analyses. This instrument has 3 channels and facilities for diffusion experiments, and includes six unique probes:

• A micro, 2.5mm Triple-Resonance Inverse TXI probe,(1H, 13C, 15N) equipped with Z-gradients
• A 5mm multinuclear, Broad Band probe in the low frequency range (BBO), 109Ag to 31P, equipped with an automatic tuning and matching (ATM) as well as Z-gradients
• A 5mm Inverse Triple-resonance probehead in multinuclear version, TBI (1H, 31P, BB) with Z-gradient. The BB range is 109Ag-31P.
• A 5mm multinuclear (109Ag-31P) Inverse probe (BBI) that includes z-gradient, an automatic tuning and matching (ATM) as well as a special tuning for 103Rh.
• A 5mm Switchable Quad probe (QNP) for 19F,31P,13C-1H-2H nuclei.
• A solid-state CP-MAS probe.

Values of pulse lengths calibrated for different nuclei on the various probes of this spectrometer are given in this site for the Weizmann Institute users.

AVANCE III-400 Bruker

The Bruker AV III-400 spectrometer located at the Sieff building (Organic Chemistry department) mainly serves scientists in the Department of Organic Chemistry and provides a modern facility for their analyses. This instrument has facilities for diffusion experiments, and includes nine probes:

• A new 5mm multinuclear (109Ag-31P, and 19F), Inverse probe (BBFI) that includes Z-gradients as well as an automatic tuning and matching (ATM)
• A new 5mm multinuclear Broad Band (109Ag-31P) probe in the low frequency range (BBO) equipped with an automatic tuning and matching (ATM) and Z-gradients.
• A 5mm proton-selective probe with highest sensitivity for biological systems and 2D measurements
• A 5mm multinuclear, Broad Band (BB) probe (103Rh to 31P)
• A 5mm automatic QNP probe, switchable by computer for 1H, 19F, 31P and 13C nuclei
• A 5mm Triple-resonance probehead in multinuclear version, TBI (1H, 31P, BB) with Z-gradient
• A 5mm multinuclear (109Ag-31P), Inverse probe (BBI) that includes Z-gradients
• A 10mm multinuclear Broad Band (BB) probe in the low frequency range, covering the range 103Rh to 31P
• A 10mm multinuclear Broad Band probe in the low frequency range (BBO), 39K-193Ir.

Values of pulse lengths calibrated for different nuclei on the various probes of this spectrometer are given in this site for the Weizmann Institute users.

AVANCE III- 300 Bruker

The low-field, UltraShield NMR instrument is used primarily for routine identification and standard work with small organic molecules. This two-channel spectrometer is located at the Sieff building (Organic Chemistry department). This is an open-access facility offered mainly to members of the Organic Chemistry department. It is equipped with the following probeheads:

• A 5mm automatic QNP probe, switchable by computer for 1H, 19F, 31P and 13C nuclei
• A 5mm multinuclear, Broad Band (BB) probe (109Ag-31P)