Nuclear
Magnetic Resonance (NMR)
Nuclear
Magnetic Resonance (NMR)(Person in Charge: Tali Scherf )
The Nuclear Magnetic Resonance (NMR) Unit comprises five High-Resolution NMR spectrometers ranging from 300-800 MHz, as well as a 400 MHz widebore spectrometer and two horizontal bore Biospec systems used mainly for imaging.
The new low-field NMR instrument (Bruker AVANCE III-300) is used primarily for routine identification and standard work with small organic molecules. Equipped with the QNP (5 mm) probe for 1H, 13C, 19F, and 31P measurements.
The new Bruker Avance III-400 spectrometer, which mainly serves scientists in the Department of Organic Chemistry, includes nine different probes (5 and 10 mm). The new BBI (5 mm) multinuclear, broad band (109Ag -31P and 19F) inverse probe, equipped with an automatic tuning and matching (ATM) as well as Z-gradients; a new BBO (5 mm) multinuclear, broad band probe in the low-frequency range (109Ag to 31P), equipped with an automatic tuning and matching (ATM) as well as Z-gradients; a selective 1H dedicated (5 mm) probe (high sensitive); a QNP (5 mm) probe, switchable by computer for 1H, 13C, 19F, and 31P nuclei; a BB (5 mm) broad band probe; a TBI (5 mm) Inverse with Z-gradient; a TXI (5 mm) Inverse (triple-channel) with Z-gradient; a BB (10 mm) multinuclear probe covering the range 107Ag to 31P; a BB (10 mm) low-frequency probe (39K -193Ir).
A Bruker AV-500 spectrometer serves mainly scientists in the Department of Organic Chemistry and provides a modern facility for their analyses. This instrument has three channels and is equipped with six uniqe probes: a micro, 2.5mm Triple-Resonance Inverse TXI probe,(1H, 13C, 15N) equipped with Z-gradients; a 5mm multinuclear, Broad Band probe in the low frequency range (BBO), 109Ag to 31P,equipped with an automatic tuning and matching (ATM) as well as Z-gradients; a 5mm Inverse Triple-resonance probehead in multinuclear version, TBI (1H, 31P, BB) with Z-gradient. The BB range is 109Ag-31P; a 5mm multinuclear (109Ag-31P) Inverse probe (BBI) that includes z-gradient, an automatic tuning and matching (ATM) as well as a special tuning for 103Rh; and a new CP-MAS probe.
The Bruker DMX-500 is mainly used for specialized research, including 2D NMR and biologically oriented work. The instrument is equipped with dedicated NMR probes for 1H, 2H, 13C, 15N, and 31P measurements, as well as for "inverse" experiments, and a 13C CP-MAS probe. It has a new, 5mm Triple-Resonance Inverse CryoProbe, TXI, (1H, 13C, 15N) equipped with Z-gradients
The 800 MHz high-resolution spectrometer (Bruker) provides access to the highest magnetic field currently available in Israel, enabling state-of-the-art high-resolution multi-dimensional experiments for macromolecular structure determination. The old DRX console was recetly upgraded to a new AVIII800 console. Its accessories include a multi-nuclear TXI probe with z gradient ( 15N, 13C, 1H, 5mm), a multi-nuclear QXI probe with x, y and z gradients ( 15N, 13C, 31P, 1H, 5mm) and a new, 5mm Triple-Resonance Inverse CryoProbe, TCI, (1H, 13C, 15N) equipped with Z-gradients as well as with an automatic tuning and matching (ATM) device.
In addition, a Bruker 400-Avance III widebore spectrometer is used for NMR microscopic imaging. Spectroscopic capabilities include 1H and broad band multi nuclei probes and an automatic QNP probe, switchable by computer. Imaging is provided by two systems: a microscopy probe includes actively shielded gradients (up to 200 G/cm) with 5 mm rf coils for 1H, 1H/ 13C and 1H/ 31P. A mini-imaging probe with actively shielded gradients (up to 150 G/cm) includes several 1H RF coils, with diameters between 4 and 30 mm. The spectrometer is used mainly for research in biology and for non-invasive physiological and metabolic measurements of small samples.
The Biospec laboratory contains two horizontal-bore imaging spectrometer: a BioSpec (Bruker) based on a 4.7 Tesla magnet with a 30 cm horizontal bore diameter. The system was upgraded during 1996 to the "Advance DBX" version with fully broadband dual-channel operation, self-shielded gradients and an assortment of resonators and surface coils with active coil detuning for crossed-coil operation. It has two interchangeable shielded X,Y,Z gradients sets, four receiver channels and parallel imaging capabilities, several 1H, 13C, 19F imaging probes (linear resonators), several 1H surface coils with various diameters (id-10, 20cm) and monitoring options of vital signs (respiration, ECG and rectal temperature). The system performs NMR spectroscopy and imaging experiments on animals, plant systems and other large and heterogeneous samples and specimens. It is being used by researchers from the Chemistry and Biology faculties for investigating tumors implanted in mice, models for angiogenesis of tumor blood vessels, spinal cord vessels, spinal cord damage and its treatment in rats, and characterizing flow and transport in three-dimensional porous media and rock fracture models.
Since March 2007, an additional horizontal-bore imaging spectrometer, Bruker Avance-III 94/20 , was installed in the new 'Mamtak' (Center for Preclinical Research) building. The magnet has a field strength of 9.4 Tesla and an accessible bore of 20 cm. It has two interchangeable shielded X,Y,Z gradients sets, four receiver channels and parallel imaging capabilities, several 1H, 13C, imaging probes (linear and quadrature resonators), several 1H surface coils with various diameters (id-10, 20cm), several multi-nuclear surface coils, body temperature control (circulating heater water) and monitoring options of vital signs (respiration, ECG and rectal temperature).