PhD Position protein NMR at Leiden University

The Leiden Institute of Chemistry (Leiden University) has an opening for a

PhD student (4 year track) on Enzyme-substrate interactions: Understanding catalysis of glycosidases at Ubbink group at the Leiden Institute of Chemistry

Project description

GH11 glycosidases are important enzymes in nature and for biotechnology. The aim of the project is to develop an approach to establish the conformation of covalent substrate intermediates in an active site and link it to the dynamics of these enzymes. Substrate analogues enriched with stable isotopes will be synthesized to enable the observation of substrate nuclei in the background of the enzyme, using nuclear magnetic resonance (NMR) and extract the conformation of the covalently bound sugar. NMR will also be used to study the effects of the adduct on enzyme dynamics with a combination of relaxation methods and paramagnetic NMR tools. The project will result in a general approach to study ligand conformations inside enzymes and yield a mechanistic description of catalysis of the GH11 family enzymes.

Selection criteria

- Master’s degree in Chemistry, Biochemistry, Biophysics or equivalent;

- Good to excellent proficiency in English;

- Ability to work independently in a multidisciplinary environment;

- Some experience with organic synthesis is required;

- Experience with NMR on proteins is a plus;

- The PhD research needs to start before December 2018.

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