Postdoc and PhD positions in structural biology of Wnt signaling, CEITEC, Czech Republic, Tripsianes Group

Postdoc and PhD positions open up immediately
Project information:
We apply structural biology methods in order to gain a mechanistic view of CK1ε action in the Wnt signalling pathways. CK1ε represents an attractive therapeutic target but currently two key steps in the CK1ε-mediated Wnt signal transduction are unclear: (PostDoc Project) how CK1ε gets activated and/or engages target proteins in response to Wnt signal and (PhD project) how CK1ε phosphorylates its key substrate DVL and what are the consequences of DVL phosphorylation.
Our preliminary data suggest that we can efficiently apply methods of integrated structural biology to (i) probe the DVL conformational landscape using in vitro and in vivo FRET sensors coupled to SAXS and CryoEM, (ii) understand the (auto)phosphorylation regulatory mechanisms of CK1ε, (iii) analyse by NMR the functional consequences of DVL phosphorylation and (iv) monitor DVL phosphorylation by real-time NMR under controlled cellular conditions.
Both positions are part of a multidisciplinary project that combines (i) cellular and molecular biology, (ii) proteomic analysis, (iii) biochemistry and structural biology, and received generous funding in a very competitive grant scheme.
CEITEC is a vibrant work place with excellent colleagues and infrastructure comparable to the best institutions in Europe for: (i) cryo-electron microscopy (Titan Krios with phase plate, Falcon3, and K2 direct electron detectors, Talos Arctica equipped with Falcon3 direct electron detector, Tecnai F20, FIB/SEM Versa3D, and vitrification robot), (ii) X-ray crystallography equipped with the BioSAXS arm on the Rigaku diffraction system, (iii) high-field NMR (500 MHz, 600 MHz, 2 × 700 MHz, 850 MHz, and 950 MHz equipped with high-sensitivity cryogenically cooled probeheads), (iv) biointeractions (SPR, ITC, DSC, MST, DSF, AUC, CD).
Requirements:
We are looking for highly motivated researchers with a strong background or interest in structural biology. Hands-on experience with protein expression and purification is prerequisite. Experience with biomolecular NMR is a plus.
Starting Date:
Immediately or upon agreement.
Employment Conditions:
Salary that will be based on the candidate’s qualifications and experience.
Contact:
Please send application (including CV, short statement of research background/interests and the contact information of at least two references) or queries by email to: kostas.tripsianes@ceitec.muni.cz

Kostas Tripsianes, PhD | CEITEC - Central European Institute of Technology | Masaryk University | Kamenice 5/A35/1S081, CZ-62500 Brno | phone: 00420 549 49 6607