New Strategies for cloning and expression of protein complexes in E. coli and insect cells

22nd - 27th, November 2009
Weizmann Institute of Science
Rehovot, Israel

Saturday - November 21st
19:00 -   Get-together off campus

Sunday - November 22nd
07:30 - 08:30   Breakfast at the San Martin Guesthouse
08:30 - 08:40   Welcome by the Head of the ISPC Prof. Joel L. Sussman and by the organizers Dr. Tamar Unger and Dr. Yoav Peleg
08:40 - 09:25   Strategies in DNA cloning (Yoav Peleg)
09:25 - 12:30   Session I: Cloning Restriction Free (RF)/ LDC and mutagenesis, Baculovirus infection
12:30 - 13:45   Lunch at the Jubilee Plaza Restaurant
13:45 - 14:30   Protein co expression in E. coli (Tamar Unger)
14:30 - 17:00   Session II: Cloning (continued) and transformation for protein expression
18:30 -   Transportation from San Martin Guesthouse to Jaffa for Tour and Dinner

Monday - November 23rd
07:30 - 08:30   Breakfast at the San Martin Guesthouse
08:30 - 10:00   Protein co expression in eukaryotic expression systems (Tamar Unger and Yoav Peleg)
10:00 - 12:30   Session III: Small scale expression in E. coli, Cloning continue
12:30 - 13:45   Lunch at the Jubilee Plaza Restaurant
13:45 - 14:30   Protein crystallization: a never ending story (Orly Dym)
14:30 - 16:30   Session IV: DNA and protein analyses tools (at the Bioinformatics Center)
16:30 - 17:30   Presentations by participants' (10 minutes each)
17:30 - 18:00   Visit to the crystallization lab at the ISPC
19:00 -   Beer, Beer and more Beer at the Dublin Pub, Rabin Science Park, Rehovot.

Tuesday - November 24th
07:30 - 08:30   Breakfast at the San Martin Guesthouse
08:30 - 09:15   Proteopedia "Life in 3D" (Jaime Prilusky)
09:15 - 12:30   Session V: Cloning, colony PCR
12:30 - 13:45   Lunch at the Jubilee Plaza Restaurant
13:45 - 14:30   Biophysical methods for detection and analysis of protein protein interaction (Gideon Schreiber)
14:30 - 16:30   Session VI: Cloning, analysis of PCR results
16:30 - 17:30   Presentations by participants' (10 minutes each)
17:30 -   Free evening

Wednesday - November 25th
07:30 - 08:30   Breakfast at the San Martin Guesthouse
08:30 - 09:15   Intrinsically unstructured proteins, IUPs (Joel Sussman)
09:15 - 12:30   Session VII: Protein extraction and purification from E. coli and insect cells
12:30 - 13:45   Lunch at the Jubilee Plaza Restaurant
13:45 - 15:30   Session VIII: Protein purification (continued)
16:00 -   Transportation to Jerusalem, tour by night including dinner.

Thursday - November 26th
07:30 - 08:30   Breakfast at the San Martin Guesthouse
08:30 - 09:15   Molecular Theater 3D in motion (Harry Greenblatt)
09:15 - 12:30   Session IX: SDF PAGE and Western blot analyses
12:30 - 13:45   Lunch at the Jubilee Plaza Restaurant
13:45 - 14:30   ISPC: From gene to 3D structure (Shira Albeck)
14:30 - 16:30   Session X: Western blot analyses (continued)
19:00 -   Farewell dinner

Friday - November 27th
07:30 - 08:30   Breakfast at the San Martin Guesthouse
08:30 - 10:00   Discussion and Summary (Tamar Unger and Yoav Peleg)
10:00 -   Checkout and Departure

sponsors

ISPC
ISPC
SPINE
SPINE