Structural Proteomics Unit

 

Zvi Livneh, Head
The Maxwell Ellis Professorial Chair of Biomedical Research

The Structural Proteomics Unit was formally established in 2010 within the Biochemistry Faculty. It hosts The Israel Structural Proteomics Center (ISPC) (http://www.weizmann.ac.il/ISPC), that was founded in 2002 as a special unit within the Faculty of Chemistry and with the support of the Ministry of Science as a Core Center for Knowledge and Service that would be concerned with protein production and structure determination. Its principal mission is to work closely with the scientific community, so as to provide a service for producing proteins and/or solving their structures. Targets are selected by the individual investigators. To this end, the ISPC has developed high throughput methodologies for cloning, expression, purification, crystallization, structure determination and structure analysis. As a result, the ISPC has had a significant impact on a large number of projects carried out by scientists at the Weizmann, as well as elsewhere. The center accepts purified proteins for crystallization, as well as providing highly purified proteins over-expressed in E. coli, P. pastoris, insect cells and human cells for crystallization and subsequent structure determination, as well as for biochemical and biophysical studies. During the past eight years, the ISPC has worked on over 300 different protein targets provided by more than 70 scientists, and solved the 3D structures of over 70 individual proteins and complexes. In order to be capable of handling effectively so many different projects in parallel, the ISPC has established a Laboratory Information Management System (LIMS), implemented state-of-the-art robotics into the workflow, and hired a staff of highly qualified personnel. The ISPC is continuously engaged in the development of new methodologies, as well as in the implementation of state-of-the-art technologies at all stages of the pipeline. The ISPC has developed extensive international collaborations, being a senior node of the EC Vth Framework Integrated Project, SPINE (Structural Proteomics in Europe (http://www.ebi.ac.uk/euprojects/index.html#spi) followed, more recently, by the VIth Framework SPINE2-Complexes Project (http://www.spine2.eu), which focused on solving 3D structures of protein complexes. It is becoming clearer that the future direction of high-throughput structural proteomics is towards solution of higher-order structures and to tight synergy with the biologists, a path that was taken by the ISPC from the very outset and the goal the European Infrastructure Project Instruct (http://www.instruct-fp7.eu), in which the ISPC serves as a core center.

Staff

Professor

Zvi Livneh1, Ph.D., Weizmann Institute of Science, Rehovot, Israel
       The Maxwell Ellis Professorial Chair of Biomedical Research

Associate Staff Scientists

Orly Dym, Ph.D., Weizmann Institute of Science, Rehovot, Israel
Yoav Peleg, Ph.D., The Hebrew University of Jerusalem, Jerusalem, Israel

Administrator

Maanit Zibziner

1Department of Biological Chemistry