DNA Sequencing

The DNA Sequencing unit provides sequencing analysis for all interested scientists. Hundreds of DNA samples are received and performed per day from scientists throughout Israel. We run cycle sequencing reactions in the presence of a dideoxy nucleotides which have been chemically modified to fluoresce under UV light (four different fluorescent colors for the four dideoxy nucleotides A, G, C, T).

Automated DNA sequencing is done using a 3730 DNA Analyzer from ABI, based on capillary electrophoresis. All users receive a colored densitometric scan which displays four different colored peaks corresponding to the different bases. Double-stranded DNA plasmids, BACs, single-stranded DNA, and PCR fragments are suitable for sequencing.

Contact

Rivi Ascarelli-Goell
Email: rivi.ascarelli-goell@weizmann.ac.il
Telephone: +972-8-934-3688/4249

More Information

All users from outside the Weizmann Institute of Science must have an active service agreement when ordering from DNA Sequencing. Visit the Biological Services homepage to find the service agreement and directions for filling it out. Then click on the link on the next line to place your order.

[Order] processing of DNA samples

W.I.S users

Access your DNA Sequencing data on susanc. Enter your Unix User Name and Password.

Other users

Access your DNA Sequencing data on noys. Enter your UserId and Password. If you do not yet have a UserId call Irit at 08-934-2470.

Click FAQ for more information about susanc and noys.


Instructions for accessing your seqfiles
Information of how to prepare your samples for proccessing