Lectures & Events

Rapid mass spectrometry investigation of overproduced proteins from crude samples

Analysis of intact proteins by native mass spectrometry has emerged as a powerful tool for obtaining insight into subunit diversity, post-translational modifications, stoichiometry, structural arrangement, stability, and overall architecture. Typically, such an analysis is performed following protein purification procedures, which are time consuming, costly, and labor intensive. As this technology continues to move forward, advances in sample handling and instrumentation have enabled the investigation of intact proteins in crude samples, offering rapid analysis and improved conservation of the biological context. This emerging approach is expected to impact many scientific fields, including biotechnology, pharmaceuticals, and clinical sciences. In my talk I will discuss the information that can be retrieved by such experiments as well as the applicability of the method by presenting the characterization of engineered proteins, drug binding, antibody specificity and protein-protein interactions. Department of Biomolecular Sciences

Chemistry colloquium

Department of Biomolecular Sciences, WIS

Chemistry colloquium

Department of Plant & Environmental Sciences, WIS