Abstract: An approach enabling the acquisition of 2D nuclear magnetic resonance (NMR) spectra within a single scan has been recently proposed. A promising application opened up by this novel ÒultrafastÓ data acquisition format concerns the monitoring of chemical transformations as they happen, in real-time. The present paper illustrates some of this potential with two examples: i) the following of an H/D exchange processes occurring upon dissolving a protonated protein in D2O and then subjecting the resulting solution to NMR observation; ii) the real-time in situ tracking of a transient Messeinheimer complex forming upon rapidly mixing two organic reactants inside the NMR observation tube. The first of these measurements involved the acquisition of train of 2D 1H-15N HSQC NMR spectra separated by ca. 4 seconds; following an initial dead-time, this allowed us to monitor the kinetics of hydrogen exchange in Ubiquitin at a site-resolved level. The second approach enabled us to observe, within ca. two seconds after the triggering of the reaction, a competition between thermodynamic and kinetic controls via changes in a series of 2D TOCSY patterns. The real-time dynamic experiments hereby introduced thus add to an increasing family of fast characterization techniques based on 2D NMR; their potential and limitations are briefly discussed.