Ultrafast Optics Group

Nonlinear Microscopy

We are studying new modalities of optical microscopy based on interaction of matter with short intense optical pulses. These techniques in general offer optical sectioning and live imaging without the need for labeling or fixing the specimens.

We have developed third-harmonic generation (THG) microscopy, which resolve structural information via spatial changes of the nonlinear susceptibility. We also employed techniques based on coherent control and optical pulse shaping for molecular imaging via coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS).

We developed techniques that use short pulse manipulation for microscopy application. Temporal focusing microscopy uses the controlled compression of the pulse to achieve scanningless nonlinear microscopy. We also study the interaction of short pulses with scattering environment such as biological tissue.

Below are our publications in the field nonlinear microscopy and spectroscopy.

	“Focusing and compression of ultrashort pulses through scattering media”, O. Katz, E. Small, Y. Bromberg and Y. Silberberg, Nature Photonics 5, 372-377,(2011) PDF “Single-pulse stimulated Raman scattering spectroscopy”, H. Frostig, O. Katz, A. Natan and Y. Silberberg, Opt. Lett.36, 1248-1250,(2011) PDF
	“Single-beam coherent Raman spectroscopy and microscopy via spectral notch shaping” O. Katz, J. M. Levitt, E. Grinvald, and Y. Silberberg Optics Express, p. 22693 (2010) PDF “Compressive Fourier Transform Spectroscopy“ O. Katz, J. M. Levitt, Y. Silberberg, arXiv:1006.2553 PDF
	“Two-dimensional phase-only spatial light modulators for dynamic phase and amplitude pulse shaping”, E. Frumker and Y. Silberberg J. Modern Opt. 56, 2049-2054, (2009) PDF ''Single-pulse standoff nonlinear Raman spectroscopy'', A. Natan, O. Katz, S. Rosenwaks and Y. Silberberg, Proceedings of Ultrafast Phenomena XVI, Springer Series in Chemical Physics , Vol. 92 (2009). PDF “Compressive ghost imaging”, O. Katz, Y. Bromberg and Y Silberberg, Appl. Phys. Lett. 95, 131110 (2009). PDF “Quantum Coherent Control for Nonlinear Spectroscopy and Microscopy” Yaron Silberberg, Annu. Rev. Phys. Chem. 2009.60 (2009) PDF “Ghost imaging with a single detector” Yaron Bromberg, Ori Katz and Yaron Silberberg, Phy. Rev. A 79, 053840 (2009) PDF
	“Shaped Femtosecond Pulses for Remote Chemical Detection”, O. Katz, A. Natan, S. Rosenwaks and Y. Silberberg, Opt. Phot. News Dec 2008, 47 . PDF “Standoff detection of trace amounts of solids by nonlinear Raman spectroscopy using shaped femtosecond pulses” O. Katz, A. Natan, S. Rosenwaks, and Y. Silberberg, Appl. Phys. Lett. 92, 171116 (2008). PDF "Phase and amplitude pulse shaping with two-dimensional phase-only spatial light modulators", Eugene Frumker and Yaron Silberberg, J. Opt. Soc. Am. B 24, 2940 (2008).PDF
	"The 3D structure of the tectorial membrane determined by second-harmonic imaging microscopy", R. Gueta, E. Tal, Y. Silberberg Y, I. Rousso, J. Struct. Bio. 159, 103 (2007). PDF "Femtosecond pulse shaping using a two-dimensional liquid-crystal spatial light modulator" E. Frumker and Y. Silberberg, Opt. Lett. 32, 1384 (2007) PDF "Broadband Sum-Frequency Generation as an efficient two-photon detector for Optical Tomography”, A. Pe’er, Y. Bromberg, B. Dayan, Y. Silberberg and A. A. Friesem, Opt. Express. 15, 8760 (2007). PDF "Dentin micro-architecture using harmonic generation microscopy", R. Elbaum, E. Tal, A.I. Perets, D. Oron, D. Ziskind, Y. Silberberg, H.D. Wagner, J. Dentistry,35 150 (2007) PDF
	"Transformation from an ultrashort pulse to spatiotemporal speckle by a thin scattering surface" E. Tal and Y. Silberberg, Opt. Lett. 31 3529-3531 PDF “Full control of the spectral polarization of ultrashort pulses”, L. Polachek , D. Oron, Y. Silberberg Opt. Lett. 31 631-633 (2006). PDF
	“Efficient polarization gating of high-order harmonic generation by polarization-shaped ultrashort pulses”, Dan Oron, Yaron Silberberg, Nirit Dudovich, and David M. Villeneuve, Phys. Rev. A 72, 063816 (2005) PDF “Femtosecond pulse-shape modulation at nanosecond rates”, E. Frumker, E. Tal, and Y. Silberberg, Opt. Lett. 30, 2796, (2005) PDF “Harmonic generation with temporally focused ultrashort pulses”, D. Oron and Y. Silberberg, J. Opt. Soc. Am. B22, 2660 (2005). PDF “Spatiotemporal coherent control using shaped, temporally focused pulses” D. Oron and Y. Silberberg, Opt. Express 13, 9903 (2005). PDF “Improved depth resolution in video-rate line-scanning multiphoton microscopy using temporal focusing”, Tal E, Oron D, Silberberg Y, Opt. Lett. 30, 1686-1688 (2005). PDF “Scanningless Depth-resolved Microscopy”, D. Oron, E. Tal and Y. Silberberg, Opt. Express 13 , 1428 (2005). PDF
	“Quantum Lithography by Coherent Control of Classical Pulses” A. Pe’er, B. Dayan, M. Vucelja, Y. Silberberg and A. A. Friesem, Opt. Express. 12, 6600 (2004). PDF “Third Harmonic generation with Cylindrical Gaussian Beams”, D. Oron and Y. Silberberg, J. Opt. Soc. Am. B 21 , 1964 (2004). PDF “All-optical processing in coherent nonlinear spectroscopy ”, D. Oron, N. Dudovich and Y. Silberberg, Phys Rev. A. 70 , 023415 (2004). PDF “Femtosecond pulse-shape modulation at kilohertz rates”, E.Frumker,D.Oron,D.Mandelik,and Y.Silberberg, Opt. Lett. 29 , 890 (2004) PDF “Confocal microscopic imaging of fast UV-laser photolysis of caged compounds” , Eduard Korkotian, Dan Oron, Yaron Silberberg, and Menahem Segal, J.Neurosc. Meth. 133 , 153 (2004). PDF
	“Depth-resolved multiphoton polarization microscopy by third-harmonic generation”, Dan Oron,Eran Tal,and Yaron Silberberg, Opt. Lett. 28 (2003). PDF “Depth-resolved structural imaging by Third-Harmonic generation microscopy”, D. Oron, D. Yelin, E. Tal, S. Raz, R. Fachima and Y. Silberberg, J. Structural Biol. (2003). PDF “Multiphoton plasmon-resonance microscopy,” D. Yelin, D. Oron, S. Thiberge, E. Moses, and Y. Silberberg, Opt. Express 11, 1385-1391 (2003). PDF “Femtosecond Phase-and-Polarization Control for Background-Free Coherent anti-Stokes Raman Spectroscopy”, D. Oron, N. Dudovich and Y. Silberberg, Phys. Rev. Lett. 90 , 213902 (2003). PDF “Single-pulse coherent anti-Stokes CARS spectroscopy in the fingerprint spectral region”, N. Dudovich, D. Oron and Y. Silberberg, J. Chem. Phys. 118, 9208 (2003). PDF
	“Single-pulse Phase-Contrast Nonlinear Raman Spectroscopy”, D. Oron, N. Dudovich and Y. Silberberg, Phys. Rev. Lett. 89 , 273001 (2002). PDF “Single-pulse Phase-Contrast Nonlinear Raman Spectroscopy”, D. Oron, N. Dudovich and Y. Silberberg, Phys. Rev. Lett. 89 , 273001 (2002). PDF “Single-pulse Coherently Controlled Nonlinear Raman Spectroscopy and Microscopy”, N. Dudovich, D. Oron, and Y. Silberberg, Nature 418 , 512 (2002). PDF “Third-Harmonic microscopy with a titanium-sapphire laser” , D. Yelin, D. Oron, E. Korkotian, M. Segal, and Y. Silberberg, Appl. Phys B (2002). PDF “Quantum Control of Coherent anti-Stokes Raman Processes”, D. Oron, N. Dudovich, D. Yelin and Y. Silberberg, Phys. Rev. A 65 , 043408 (2002). PDF “Narrow-Band Coherent Anti-Stokes Raman Signals from Broad-Band Pulses ” , D. Oron, N. Dudovich, D. Yelin and Y. Silberberg, Phys. Rev. Lett. 88 , 63004-1 (2002). PDF
	“Third Harmonic Microscopy” , D. Yelin and Y. Silberberg, Microscopy and Analysis, Nov. 2000.
	“Laser scanning third-harmonic-generation microscopy in biology,” D. Yelin and Y. Silberberg, Opt. Express, 5, 169 (1999). PDF “Coherent Quantum Control of Multiphoton Transitions by Shaped Ultrashort Optical Pulses”, D. Meshulach and Y. Silberberg, Phys. Rev. A., 60, 1287 (1999). PDF “Phase-Matched Third-Harmonic Generation in a Nematic Liquid Crystal Cell”, D. Yelin, Y. Barad, J. S. Patel and Y. Silberberg, Phys. Rev. Lett., 82, 3046-3049, (1999). PDF “Depth-Resolved Imaging of Nematic Liquid Crystals by Third Harmonic Microscopy”, D. Yelin, Y. Barad, J. S. Patel and Y. Silberberg, Appl. Phys. Lett., 74, 3107 (1999). PDF
	“Coherent Quantum Control of Two-Photon Transitions by a Femtosecond Laser Pulse” , D. Meshulach and Y. Silberberg, Nature, 396, 239-242 (1998). PDF
	“Measurement of Ultrashort Optical Pulses by Third Harmonic Generation”, D. Meshulach, Y. Barad, and Y. Silberberg, J. Opt. Soc. Am. B, 14, 2122 (1997). PDF “Nonlinear Scanning Laser Microscopy by Third Harmonic Generation”, Y. Barad, E. Eisenberg, M. Horowitz, and Y. Silberberg, Appl. Phys. Lett. 70, 922 (1997). PDF