Intravital 2-photon Movies

  • Three-dimensional reconstruction of the inguinal lymph node, showing YFP-CD11c DCs (green) and the collagen in the capsule (blue)
  • OVA-specific (cyan) and control (blue) T cells move among DCs (Green) around the high endothelial venules in a live lymph node
  • T cells specific for OVA (yellow) cluster within a DC network (green) after antigen challenge
  • DCs (green) traversing a B cell follicle (blue cells) and "probing" the perifollicular region
  • T cells (blue) and DCs (green) moving among blood vessels in the lymph node
  • Three-dimensional reconstruction based on the data from the previous movie
  • GFP+ T cells, flow among endogenous lymphocytes in an efferent lymphatic, note lymphatic valve at lower right
  • Tracking the patrolling movement of dermal DCs
  • A single dermal DC crawling in the dermis
  • Tracking a single T cell in the lymph node
  • Tracking multiple T cells