Ludmila Frolov - CV and Publications

Research

     RNA performs fundamental biological functions when organisms such as bacterial and viral pathogens replicate. Developing small molecules that can interact with bacterial RNA and interrupt cellular activities is a promising path for drug design. Among the natural products that recognize RNA specifically, the class of aminoglycoside (AMG) antibiotics stands out. AMGs exert their biological functions by binding to prokaryotic ribosomal RNA and interfering with protein translation, resulting in bacterial cell death. The decoding site, a small internal loop within the 16S rRNA, is the molecular target for the naturally occurring AMG antibiotics.

     The key element of my research is the development of bio-recognition interfaces comprised of functional receptor (thiol- or disulfide-derivatized aminoglycoside and guanidinoglycoside molecules) monolayers on gold island films, providing label-free optical transducers capable of monitoring receptor-analyte interactions. The system will be applied to the study of the interactions of aminoglycoside antibiotics and their derivatives with the ribosomal decoding RNA site, as well as the interactions of guanidinoglycoside molecules with cell surface glycosaminoglycans and mammalian cells for future usage as biosensor.

CV

EDUCATION

2003-2007 Ph.D. student in the direct Ph.D. program Department of Biochemistry,
Faculty of the Life Sciences,Tel Aviv University, Israel

Thesis advisor: Prof. C. Carmeli
Thesis title: "Self-assembly of oriented monolayer from photosystem I on solid gold surface"
Summa Cum Laude

2001-2003 M.Sc. Department of Biochemistry,
Faculty of the Life Sciences, Tel Aviv University, Israel, Supervisor: Prof. C. Carmeli

1995-1999 B.Sc. School of Chemistry, Faculty of the Exact Sciences, University of Buryatia, Russia

EMPLOYMENT EXPERIENCE

2009-present Postdoctoral fellow Department of of Materials and Interfaces, Faculty of Chemistry, Weizmann Institute of Science, Rehovot, Israel, Supervisor: Prof. I. Rubinstein
"Direct Observation of Aminoglycoside-RNA Interactions by Localized Surface Plasmon Resonance Spectroscopy"

2007-2009 Research Associate Department of Biochemistry, & Institute for Nanoscience and Nanotechnology Tel Aviv University, Supervisor, Prof. C. Carmeli, "Design and construction of new bio- photovoltaic devices".

2003-2007 Teaching assistant, Department of Biochemistry, Tel Aviv University.

2002-2003 Laboratory assistant Department of Biochemistry, Tel Aviv University Laboratory.

 

 MAJOR FIELDS OF TRAINING AND RESEARCH EXPERINCE

Molecular biology and genetics techniques: PCR, plasmid construction and analysis, site-genetic, RNA synthesis.
Biochemical techniques: Isolation of membrane proteins, affinity chromatography, ion exchange, immunoprecipitation, dialysis, gradient fractionation, western blots, biochemical assays.
Spectroscopy: Time resolves laser spectroscopy, X-ray absorption spectroscopy, UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR).
Microscopy: Atomic Force Microscope (AFM), Kelvin Probe Force Microscope (KPFM), optical microscope, High-Resolution Scanning Electron Microscopy (HRSEM)
Surface chemistry: mono (multi) layers fabrication, self-assembly, spin-coating, electrochemical deposition, nano-particle hybrid system.
Metals deposition: e-beam vacuum evaporator (VST).
Electrical characterization: Current-Voltage measurement probe station.
Data and image processing.

ACADEMIC AWARDS

  1. (2009) Clore Postdoctoral Fellowship, Weizmann Institute
  2. (2007) Scholarship for excellence in teaching and research, from the Life Sciences Faculty, Tel Aviv University.
  3. (2007) Mena Travel Fellowship for promising Ph.D. students.
  4. (2004) Scholarship for excellence in teaching and research, from the Life Sciences Faculty, Tel Aviv University.

PUBLICATONS

  1. Frolov, L.; Rosenwaks, Y.; Carmeli, C.; Carmeli, I. Fabrication of Photo-Electronic Device by Direct Chemical Binding of the Photosynthetic Reaction Center Protein to Metal Surfaces. Adv. Mater. 2005, 17, 2434-2437.
  2. Frolov, L.; Carmeli, I.; Carmeli, C.; Richter S. Photovoltaic Activity of Photosystem I-Based Self-Assembled Monolayer. J. Am. Chem. Soc. 2007, 129, 12352-12353.
  3. Frolov, L.; Wilner O.; Carmeli, C.; and Carmeli I Fabrication of Oriented Multilayers of Photosystem I Proteins on Solid Surfaces by Auto-Metallization. Adv. Mater. 2007, 20,2, 263-266.
  4. Carmeli, I.; Mangold M.; Frolov, L.; Zebli B.; Richter S.; Holleitner A. A Photosynthetic Reaction Center Covalently Bound to Carbon Nanotubes. Adv. Mater. 2007, 19, 3901-3905
  5. Frolov, L.; Rosenwaks, Y.; Richter, S.; Carmeli, C.; and Carmeli, I. Photoelectric Junctions Between GaAs and Photosynthetic Reaction Center Protein. J. Phys. Chem. C 2008, 112,13426 – 13430
  6. Sepunaru Lior; Tsimberov Irena; Frolov Ludmila; Carmeli Chanoch; Carmeli Itai; Rosenwaks Yossi Picosecond electron transfer from photosynthetic reaction center protein to GaAs. Nano Lett. 2009; 9 (7): 2751-2755.
  7. Frolov, L.; Dix, A.; Tor, Y.; Tesler, A.B.; Chaikin, Y.; Vaskevich, A.; Rubinstein, I.; Direct Observation of Aminoglycoside–RNA Binding by Localized Surface Plasmon Resonance Spectroscopy. Anal. Chem. 2013, 85, 2200-2207.

PATENTS

  1. PCT/IL2006/000241, FABRICATION OF PHOTO-ELECTRONIC DEVICE BY DIRECT CHEMICAL BINDING OF THE PHOTOSYNTHETIC REACTION CENTER PROTEIN TO METAL SURFACES. Carmeli, I, Carmeli, C., and Frolov, L. 2005.
  2. PCT/IL2007/001045, OPTOELECTRONIC DEVICE AND METHOD OF FABRICATING THE SAME,(Metal Based). Carmeli, I, Carmeli, C., Richter, S. and Frolov, L. 2006.
  3. PCT/IL2007/001046OPTOELECTRONIC DEVICE AND METHOD OF FABRICATING THE SAME (Semiconductor Based). Carmeli, Carmeli, C., Rosenwaks, Y., I. and Frolov, L. 2007.
  4. Provisional 2007108-00-00, OPTOELECTRONIC DEVICE AND METHOD OF MANUFACTURING SAME (Enhancement). A. Govorov, I. Carmeli, C. Carmeli, L. Frolov, S. Richter, Y.Rosenwaks, 2007.
     
Last Updated: 04/19/2024 - 16:13 | mariano.susman@weizmann.ac.il