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Dafna Geblinger |
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The effect of substrate properties on the structure, dynamics and activity of the resorption apparatus of osteoclasts |
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Bone is composed of collagen fibrils mineralized by carbonated apatite crystals that grow in the gap regions of the fibers. During maturation, mineralization increases, resulting in changes in the chemical and mechanical properties of bone. Bone is a dynamic tissue in the sense that it is constantly being repaired and remodeled by osteoclasts (bone-degrading cells) and osteoblasts (bone-depositing cells). Excessive or deficient activity of osteoclasts can lead to pathological conditions such as osteoporosis and osteopetrosis, respectively.
Since the primary role of osteoclasts is to degrade bone, it is conceivable that their activity is regulated by signals induced by the surface. The signals governing osteoclast adhesion and activation, followed by cessation of resorptive activity and migration to a new site, are not clear yet.
We examine the structure, dynamics and activity of the osteoclast adhesion and resorption apparatus on different substrates. Determining the properties of the surface that osteoclasts can sense, and examining the way these properties affect the structure and function of the osteoclast resorption apparatus, could lead to better understanding of osteoclast function in general as well as in pathological conditions.
This work is conducted in collaboration with Prof. Lia Addadi Dept. of Structural Biology, The Weizmann Institute of Science.
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Osteoclast on calcite. Mechanical removal of the osteoclast, using micromanipulation, reveals a resorption pit. The resorption pit under the cell reveals mineral spikes, typical for acid dissolution of calcite. Notice the organic matrix left on the calcite spikes. |
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