Ron Milo
Please check out my lab's website at the Weizmann institute in Israel.
e-mail: ron.milo at weizmann.ac.il
phone: +972-505-714697
You can check my website in Boston at: http://openwetware.org/wiki/User:Ronmilo
Research interests:
Plant and environmental systems biology
I want to harness the tools and approaches employed in systems biology to bear on the grand
challenges of sustainability. I am studying the efficiency of photosynthesis, trying to gain
insight about the constraints that shape its properties and the limitations on the maximal
productivity in plants and other photosynthetic organisms. In the process of studying plants
I developed a tool for the automatic measurement of hypocotyls (stems) and roots -
HypocoTool .
Dynamic proteomics
I am interested in studying the dynamics of protein levels at the single cell level.
This promises to give us a deeper understanding into biological processes.
In a research effort with Alex Sigal and other members of the Alon group at the Weizmann Institute
I am developing an experimental tool for dynamic proteomics in individual living human cells.
This method enabled us to measure the variability and temporal memory in thousands of cells for
several dozen proteins. Our approach uses a fluorescently tagged library under endogenous
regulation analyzed using time lapse microscopy and custom written image analysis tools.
How physiological adaptations affect evolutionary adaptations
A central issue in understanding natural selection is the relationship between physiological
adaptations and evolutionary adaptations. Though extensively discussed in qualitative terms,
quantitative analysis has been lacking. Under the guidance of Marc Kirschner and Michael Brenner
I am studying this relationship using hemoglobin as a model system, relying on extensive
experimental data measured for various organisms and under varying conditions.
Network motifs - building block of complex networks
To understand biological networks, together with Uri Alon and members of his lab,
we have defined "network motifs": interaction patterns that appear in a network much more
than expected in random. Network motifs help in finding functional building blocks
of complex networks. Network motifs have been found to perform information processing
tasks by studying their temporal dynamics in microorganisms.
The approach was used to classify networks into superfamilies.
BioNumbers database
This is a collaborative effort to establish a database of useful biological numbers
such as the number of ribosomes in the cell, the volume of the nucleus,
the rate of translation and transcription and many many other useful but too often hard
to find biological numbers. You can learn more about it and check out the current
version at the BioNumbers database.
Optimality in biology
A subject I am fascinated with. Here is a compilation of annotated examples I am compiling
- Optimality in biology collection.
Here is my CV.
Selected Publications:
-
Ron Milo, Jennifer H. Hou, Michael Springer, Michael P. Brenner, and Marc W. Kirschner
The relationship between evolutionary and physiological variation in hemoglobin
PNAS 2007 104: 16998-17003.
- A. Sigal*, R. Milo*, A. Cohen*, N. Geva-Zatorsky, Y. Refaeli, , T. Danon, N. Pertzov & U. Alon,
Variability and memory of protein levels in human cells.
Nature 444(7119) 643-6 (2006) [PDF]
* these authors contributed equally to this work
- A. Sigal*, R. Milo*, A. Cohen, N. Geva-Zatorsky, Y. Klein, I. Alaluf, N. Swerdlin, N. Perzov, T. Danon, Y. Liron, T. Raveh, A. E. Carpenter, G. Lahav & U. Alon,
Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins.
Nature Methods 3, 525 - 531 (2006) [PDF]
* these authors contributed equally to this work
- R. Milo, S. Itzkovitz, N. Kashtan, R. Levitt, S. Shen-Orr, I. Ayzenshtat, M. Sheffer & U. Alon,
Superfamilies of designed and evolved networks
Science, 303:1538-42 (2004) [PDF]
- R. Milo, S. Shen-Orr, S. Itzkovitz, N. Kashtan, D. Chklovskii & U. Alon,
Network Motifs: Simple Building Blocks of Complex Networks
Science, 298:824-827 (2002) [PDF]
- S. Shen-Orr, R. Milo, S. Mangan & U. Alon,
Network motifs in the transcriptional regulation network of Escherichia coli
Nature Genetics, 31:64-68 (2002) [PDF]