Alon's lab
To understand the cell, it is essential to observe its thousands of proteins asthey are created, interact and move about the cell, and are finally degraded. For thispurpose, we are developing a novel system for seeing the positions and amounts ofthe different proteins over time in living human cells. The system is based on a libraryof cell lines (LARC), each with a different protein that is fluorescently marked andexpressed from its normal chromosomal location with its normal regulation. In thepast year, we have tagged several hundred proteins and probed biological phenomenathat are very difficult to measure in multiple proteins when using any other approach:how long cells remember, and how proteins behave over the cell cycle. We also madeimportant advances in imaging, quantifying protein behavior and library generation,towards our goal of capturing the majority of the cells' proteins in response to specific drugs.
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Oscilations of P53 and MDM2 |
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