Kam's lab
High-resolution light-microscopy and high-throughput screening are two essential methodologies for characterizing cellular phenotypes. Optimally combining these methodologies in cell-based screening to test detailed molecular and cellular responses to multiple perturbations constitutes a major challenge. We developed a screening microscope platform that automatically acquires and interprets sub-micron resolution images at fast rates. In parallel to hardware development, including optics, electronics and computerized control, we assembled a powerful set of computerized analysis programs, in order to process Terabytes of the acquired image data, quantify multiple subcellular features and statistically compare their distributions in treated vs. control cells. The new computer cluster presently installed in our floor will be used for the analysis of the data. This system allow designing high throughput screening (HTS) experiments involving thousands of live cell samples, and is being applied to a number of projects at the Weizmann Institute. The results yield direct information about cellular processes and molecular mechanisms mediating complex cellular functions.
Automated microscope and robotic system
for sample preparation and high-throughput
screening
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