RNA emerges in recent years as a dominant mechanism for regulation of biological processes at all levels.
The aim of this course is to explore advanced topics in RNA-based regulation.
A strong focus is put on microRNA biochemistry, target selection and developmental impact, small RNA based regulation in prokaryotes including riboswitches, sRNA, and CRISPR.
The course will be substantiated by a set tutorials and a test, endowing 2.5 credit points.
Introduction to microRNA and the Classic Works of Ambros and Ruvkun.
Posttranscriptional Processing of microRNA.
microRNA:Target Pairing and RISC Function.
miRNA Target Genomics.
microRNA Function in Vertebrate Development.
On miRNA in Plants.
OncomiRs and Tumor Suppressor miRNAs.
Small Bacterial RNAs -CRISPR.
Additional Topics Discussed.
Alternative Splicing.
Nonsense Mediated RNA Decay.
RNA Editing.
RNA Secondary Structure.
Bacterial ncRNAs and Riboswitches.