10. Laboratory Practice and Technique

The most important element of containment is the strict adherence to standard microbiological practices and techniques. Persons working with infectious agents or potentially infected materials should be aware of the potential hazards, and must be trained until proficient in the practices and techniques required for handling such material safely. The director or the person in charge of the laboratory is responsible for providing or arranging for the appropriate training of personnel.

Each laboratory should operate according to a biosafety or operations manual which identifies the hazards that will or may be encountered, and which specifies practices and procedures designed to minimize or eliminate the risks. Personnel should be advised of the special hazards and should be required to read the manual and follow the required practices and procedures. A scientist trained and knowledgeable in the appropriate laboratory techniques, safety procedures, and hazards associated with handling infectious agents should direct laboratory activities.

When standard laboratory practices are not sufficient to control the hazard associated with a particular agent or laboratory procedure, additional measures may be needed. The laboratory director is responsible for selecting additional safety practices, which must be in keeping with the hazard associated with the agent or procedure.

Laboratory personnel, safety practices and techniques must be supplemented by the appropriate facility design and engineering features, safety equipment, and management practices.