14. Detailed Biosafety Level 1 Requirements (BL1)

Standard Microbiological Practices. No Human Pathogens Access

• Keep the laboratory doors closed when experiments are in progress.
• Report any evidence of rodent or insect infestations.

Personal Protection

• Do not eat, smoke, drink, or apply cosmetics.
• Wear gloves, lab coats as appropriate.
• Remove gloves, lab coats when leaving to non-lab areas.
• Wash hands after completing experimental procedures and before leaving the laboratory.

Work Practices

• Do not mouth pipette. Use mechanical pipetting devices.
• Minimize the use of needles.
• Design procedures to MINIMIZE aerosol formation.
• Keep work surfaces uncluttered to facilitate spill clean-up.

Decontamination Procedures

• Disinfect laboratory work surfaces daily, and immediately after a spill.
• Contaminated liquid or solid waste must be docontaminated and disposed of, according to waste disposal regulations. (Refer to Section 19.)

Examples of BLl work

• E coli K12
• Continuous culture of many established cell lines