Biology
- Which projects require approval before the work begins?
- Bacteria, viruses, fungi, parasites or their products which require biosafety level 2 or higher containment
(See CDC BMBL http://www.cdc.gov/od/ohs/biosfty/bmbl/bmbl-1.htm.)- Non-exempt recombinant DNA research
- Human gene transfer-IBC approval must be obtained from each institution at which recombinant DNA material will be administered to human subjects
(See Appendix M of the NIH guidelines for recombinant DNA: http://www4.od.nih.gov/oba/rac/guidelines/guidelines.html.)
- The BSC has a UV lamp. Can I use it?
The use of UV lamps is NOT recommended in BSCs. UV lamp switches should be integrated so that the UV lamp is off when the BSC is in use. If left on while working, UV light can burn the cornea(s) and skin. Users should clean UV lamps weekly to remove dust and dirt and test effectiveness them periodically to ensure germicidal effectiveness.Click here for the UV Fact Sheet.
Chemistry
- What is an escape mask and how is it used?
- How does one work safely with Ethidium bromide?
- Have the MSDS ready in the lab.
- Use nitrile gloves.
- Discard waste in separate labeled containers to departmental chemical waste collection area.
Waste Disposal