BIOINFORMATICS<-->STRUCTURE
Jerusalem, Israel, November 17-21, 1996

Abstract

Computer analysis of nucleotide sequences from the family of interspersed repetitive elements MER1. Another oligonucleotide primer for the PCR assay of human DNA

A.A.Ptitsyn (1), E.I.Jantsen (2), M.L.Filipenko (1), O.A. Baturina (1), A.I.Muravlev (1), N.P.Mertvetsov (1)

(1) Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Lavrentjev Prospect 10, Novosibirsk 630090, Russia
(2) Novosibirsk institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Lavrentjev Prospect 8, Novosibirsk 630090, Russia

ptitsyn@mailhost.bionet.nsk.su

We have explored a possibility of using oligonucleotides from the family MER1 of interspersed repeats as PCR primers for amplification of human genomic DNA. Those we are now recommending have been selected from release EMBL databank of nucleotide sequences by means of computer analysis. Computer selection is based on the distance measure between oligonucleotides, adopted for the PCR model. Computer program uses a dynamic algorithm and able to select distinguishing long oligonucleotides in whole EMBL subbases (like human and rodent sets) in resonable time. We have also demonstrated the use of one of the oligonucleotides in specific amplification of human DNA .

Back to the Abstract Index.