Advanced Services|Transgenics & Knockouts

TG banner

Genetically engineered mouse models are essential tools for elucidating the mammalian genome and as models for human disorders. The Transgenic and Knockout Unit is dedicated to producing genetically modified mice via transgenic and gene targeting methodologies, using state of the art techniques. Our services include the production, maintenance, rederivation, genotyping, importing, and archiving of genetically modified mice.

 

Core services:

  • Generation of gene-targeted knockout and knock-in mouse lines using CRISPR/Cas9 technologies,
    via direct injection into one-cell mouse embryos

  • Generation of transgenic mice, via DNA microinjection into one-cell embryos.

  • Generation of gene-targeted knockout and knock-in mouse embryonic stem (ES) cell lines

  • Generation of gene-targeted knockout and knock-in mice via ES morula aggregation or blastocyst injection

  • Line archiving - sperm and embryo cryopreservation

Support services:

Assisted fertility

  • IVF

  • Artificial insemination

  • Ovary transfer

Preimplantation embryos

  • Embryo flushing for: lentiviral infection, establishing new cell lines, genotyping, embryo transfer etc

Mouse surgical manipulations

  • Vasectomy, ovariectomy, blood collection, IV injections etc.

Import/Export of New Strains

  • Import/export of new strains from fresh preimplantation embryos

  • Import/export new strains from cryopreserved embryos

  • Import/export new strains from cryopreserved sperm

Rederivation of mouse lines

  • Rederivation via embryo transfer or in vitro fertilization using sperm

Stem Cells

  • Chimera formation from targeted ES lines from ES consortia

  • De novo establishment of new ES lines

  • Tetraploid aggregation to investigate maternal effect mutations

Consultation

  • Consultation, technical support and training