Transgenic & Knockout Facility

Embryonic Stem Cell Lab Transgenics & Knockouts Cryopreservation Services & IVF
Histology & Pathology Immunology Lab Microscopy & Digital Imaging

Cryopreservation of Mouse Strains

Cryopreservation of mouse strains serves two important functions: The cryopreservation service preserves mouse lines by sperm freezing.

At least 20 straws of sperm from three male mice are stored in two separate locations (note; males are sacrificed for sperm collection). Quality control of frozen sperm is assessed by thawing one straw of frozen sperm for IVF, with wild-type oocytes. The resulting embryos are transferred to pseudopregnant females to ensure they produce viable offspring, at least till E14.5. Embryo tissue samples are provided to the client for PCR verification of the strain.

Ordering

In order to preserve a line the following is required:

Contact Golda Damari (3221) with details of your order.

Important: When transferring the cages of male mice for freezing, the order number must be clearly written on the cage label.

In the case of complicated genotypes (> one mutation), or if there is strain information we should be aware of, please contact me before initiating the order.

In the event of a waiting list for freezing, we appreciate your patience and cooperation.

In Vitro Fertilization (IVF)

In vitro fertilization may be used as an alternative in lines with breeding difficulties. Contact:

Dr. Rebecca Haffner
Phone: 2719
Email: rebecca.haffner@weizmann.ac.il