|Embryonic Stem Cell Lab||Transgenics & Knockouts||Cryopreservation Services & IVF|
|Histology & Pathology||Immunology Lab||Microscopy & Digital Imaging|
Contact: Dr. Rebecca Haffner
ES cells, mouse embryo fibroblast (MEF) feeder cells, tissue culture media, growth factors and selection reagents will be supplied as well as all necessary tissue culture and transfection facilities. Transfection and selection for recombinant clones will be carried out by the investigator. DNA analysis of selected clones will be carried out in the investigator's own lab.
Early passage, germ line proven RI ES cells from (129/Sv x 129/Sv-CP)F1mice, http://www.mshri.on.ca/nagy/r1.htm, established by Andeas Nagy are available. These cells are competent for aggregation and blastocyst injection.