Transgenic & Knockout Facility

Embryonic Stem Cell Lab Transgenics & Knockouts Cryopreservation Services & IVF
Histology & Pathology Immunology Lab Microscopy & Digital Imaging Price List

The following table contains the Price List for Services at the Genetically Modified Animals Facility.
Note that all fees are in US$.

Pathology, Histology and Embryology:

Service Fee for
Weizmann Users
Price for
Academia Outside the Institute
Price for
Non-Academic Users
Notes
Autopsy of one male and one female mouse no charge $100   Includes two controls and an initial set of survey slides
Hematologic evaluation, per mouse (by an outside lab) charged directly      
Paraffin block $12 $20    
Paraffin section – unstained or HE/slide $1.20 $2    
Paraffin section – special stains upon request upon request    
Plastic block (Historesin ®) $20 $40    
Plastic section (single) $3 $4    
Histology technician/hour $50 $50    
Immunohistochemistry under review under review    
Cryostat sections under review under review    
X-ray/hour $10 $25    
Confocal microscope $10 $40 $100  
Confocal microscope with tuition $10 $80 $140  

The policy is to train scientific personnel without charge how to use equipment provided by the Facility.

Transgenic and Knockout Services:

Service Fee for
Weizmann Users
Price for
Academia Outside the Institute
Price for
Non-Academic Users
Notes
ES cell transfection reagent costs $500 + reagent costs $1,000 + reagent costs  
Transgenic mouse production $500 $2,800 $4,000 Guarantees two founders or transfers to 20 females
Production ES chimeras- aggregation $500 $2,300 $4,000 Guarantees three chimeras or transfers to 15 females
Production ES chimeras- blastocyst injection $500 $2,800 $4,000 Guarantees three chimeras or transfers to 8 females
Cryopreservation of embryos (including test thawing) $500 $2,000 $2,000 500 – 2000 embryos frozen
Cryopreservation of embryos (not including test thawing)   $1,000 $1,000 As above
IVF $350      
Congenics - superovulated $930      
Isolating embryos > 9.5d
Price per litter
$10 $20    
Isolating embryos < 9.5d
Price per litter
$20 $40    

The unit will accept only registered experiments. DNA for TG experiments must be prepared according to the handout. DNA will be investigated by the unit for concentration and quality. For targeting experiments, ES cells prepared at the ES cell lab at the GMAF are preferred. ES cells, fibroblast feeder cells, tissue culture media, growth factors and selection reagents will be supplied as well as transfection facilities. The investigator will undertake the actual transfection and selection experiment to generate clones. DNA analysis of selected clones will be carried out in the investigator's own lab.

The charge for TG and KO production is per session and includes: test of DNA purity and concentration, purchase, maintenance, hormonal injections, microinjection/aggregation, and embryo transfer to female receipients. In a typical session, microinjected or aggregated embryos are transferred to 20 pseudopregnant females. Under these conditions it is expected that at least 3 transgenic founders or chimeras will be produced. The mice are kept at the unit until the chimeras or the transgenic generation is born. Thereafter, the TG or KO mice are transferred to the regular animal rooms and the department's maintenance and purchase costs apply.