Introduction
The Genomics Unit at the INCPM provides next-generation sequencing services using the Illumina technology platform and currently Illumina sample preparation kits. We offer a modular but all-inclusive service starting from project design consultation over sample preparation, sequencing and data analysis (in collaboration with the bioinformatics unit).
Our expertise will be to provide a panel of “home-made” sample preparation protocols, which allow for higher flexibility and reduced sample input while significantly reducing overall sequencing costs compared to commercially available kits. In addition to the sequencing service there will be an ongoing effort to develop new applications and protocols to serve the needs of the scientific and medical community.
Genomics Unit transition period
Please realize that, with regards to the Genomics Unit, we are in a transition period necessary to train new staff, build new labs from scratch in the Solar Tower building, while at the same time working on historical as well as new projects and processes.
Services and Prices
Sample Preparation
We currently provide sample preparation using Illumina sample preparation kits. Please find the application and prices in the table below:
| Application | Price/Sample [NIS] |
|---|---|
| DNA-Seq | 1200 |
| RNA-Seq | 1200 |
| ChIP-Seq | 1800 |
| smallRNA-Seq | 1200 |
Sequencing Runs
Sequencing runs are performed on the Illumina HiSeq2000. Please see the run options and prices in the table below:
| Run Description | Run mode | Price /Lane [NIS] |
|---|---|---|
| Single reads 50bp | SR 50 | 4500 |
| Single reads 100bp | SR 100 | 6215 |
| Paired End reads 2* 50 bp | PE 2×50 | 7380 |
| Paired End reads 2* 100 bp | PE 2×100 | 10500 |
Sample Submission Criteria
DNA
The DNA sample to be processed should be highly pure, having an OD260/280 ratio between 1.8 and 2.0.
RNA
Please submit your total RNA sample in solution resuspended in DEPC-treated RNase-free water.
Application specific requirements
Pleasefind the application specific requirements below. If you have questions regarding sample submission please contact the lab.
| Application | Input | Amount [ μg] | Volume [ μl] | Concentration [ng/ μl] |
|---|---|---|---|---|
| DNA-Seq | gDNA | 2 | 50 | 40 |
| RNA-Seq | total RNA | 1 | 50 | 20 |
| ChIP-Seq | ChIP enriched DNA | 0.02 | 20 | 1 |
| smallRNA-Seq | total RNA | 1 | 50 | 20 |
Run Schedules
Short read runs are performed at a minimal frequency of once every two weeks. If a flow cells become full before the end of two weeks, an earlier run will be scheduled. Paired end read runs are performed at a minimal frequency of every month. Similarly, higher frequency will be based on demand.
For specific schedules – please visit our schedule page.
NGS sequencing Lab
Levine Building, 2nd floor, room 202
Tel: 08-934 4907
NGS R&D lab
Currently located at Stone building – room 502
Tel: 08-934 5168