An ERC-funded 3.5-year PhD position on solid-state and solution NMRof protein complexes in the Lewandowski group at University of Warwick, Coventry, UK

PhD position is available in the group of Dr Józef Lewandowski in Chemistry Department at the University of Warwick, Coventry, UK with a start date in Oct 2015. Application deadline 30 June 2015.

Description

This PhD project involves applications of solution and solid-state NMR to study structure and dynamics of protein complexes. In particular, emphasis will be placed on working with complexes from polyketide synthases, which are multicomponent enzymatic assembly lines for a range of useful natural products, for example, antibiotics that are effective against certain multidrug resistant infections. The aim is to use the obtained biophysical insights to facilitate rational engineering of polyketide synthases for synthetic biology applications.

The project will involve overexpression of isotopically labeled proteins, characterization of biomolecular systems by solution and solid-state NMR as well as other biophysical techniques and to lesser extent NMR method development.

This PhD project is a part of larger project involving several interdisciplinary collaborations providing an excellent opportunity for exposure to a wide range of biophysical, molecular and synthetic biology methods.

Warwick Chemistry is one of the top UK Chemistry Departments (further details at : http://www2.warwick.ac.uk/fac/sci/chemistry/). The project will be conducted at the Warwick Laboratory for Magnetic Resonance (http://www2.warwick.ac.uk/fac/sci/physics/research/condensedmatt/nmr/), which is a world-class biological and material science magnetic resonance set up hosting 7 academic staff from Physics and Chemistry and housing a suite of 8 solid-state NMR spectrometers (from 100 to 850 MHz), 2 dynamic nuclear polarization (DNP) spectrometers (94 and 200/400 GHz) and advanced EPR instrumentation. A wide range of magic angle spinning probes is available at the laboratory including number of 1.3 mm probes (spinning frequencies up to 67 kHz) and 0.8 mm probe (spinning frequencies up to 100 kHz). Solution NMR facilities include 500, 600 and 700 MHz spectrometers (http://www2.warwick.ac.uk/fac/sci/chemistry/research/facilities/nmr/). The project will also benefit from access to a soon-to-arrive dedicated 700 MHz solid-state NMR spectrometer and a Chemical Biology Research Facility providing cutting edge infrastructure for organic synthesis, molecular biology, protein chemistry, microbiology, radiochemistry and biophysical/biochemical analysis.

More info on the Lewandowski group can be found at: http://www2.warwick.ac.uk/fac/sci/chemistry/research/lewandowski/lewandowskigroup
The start date is Oct 2015.

Funding

This studentship provides 3.5 years of full funding for UK/EU students. Current stipend rate is £14210 pa.

Requirements

Motivated applicants should have (or be about to receive) an honours degree (at least 2.1 in the UK system or equivalent) in chemistry, physics or biology. Excellent writing and communication skills in English are required.

How to apply?

Motivated candidates should send a CV and cover letter to Dr Józef Lewandowski at j.r.lewandowski@warwick.ac.uk

Application deadline is 30 June 2015.