PhD position on biomolecular assemblies by solid-state NMR - Bordeaux

Laboratory: http://www.loquetlab.org/

Our laboratory is fully equipped for molecular biology, protein expression and purification. We manage the NMR platform of the IECB/CBMN, including 300, 500, 600, 700 and 800MHz NMR spectrometers equipped with solid-state NMR probes.

 

Description: The IECB (Bordeaux, France) is an international research center at the interface between biology and chemistry, equipped for structural biology with NMR, mass spectrometry, X-ray crystallography and cryo-EM platforms. Our laboratory “NMR of Molecular Assemblies” is interested in structures and molecular interactions in large biological assemblies and aims at understanding the assembly mechanisms and the structure-function interplay of complex macromolecular assemblies. In particular, we develop and apply state-of-the-art solid-state NMR spectroscopy to decipher the atomic architectures and interactions of insoluble and non-crystalline protein assemblies.

 

This ERC-funded project is focused on exploring the role and the assembly mechanism of several functional amyloids involved in complex biological processes such as programmed cell death or bacterial infection. We will use solid-state NMR spectroscopy to decipher the architecture, ultimately at atomic resolution, of challenging functional amyloid assemblies. This project is performed in close collaboration with experts on the biology of these systems to fully understand the functional aspects of amyloids, integrating in vivo and structural information on these complex biological processes.

 

Advantageous expertise includes: interest in structural biology, experience in production and purification of proteins and / or experience in solid-state or solution NMR.

 

Highly motivated candidates should send applications including CV and names of two references to Antoine Loquet (a.loquet@iecb.u-bordeaux.fr) and Birgit Habenstein (b.habenstein@iecb.u-bordeaux.fr).

 

Starting date: Available

 

Selected publications:

Stanek et al., Angew Chem Int Ed Engl. 2016

Daskalov et al., Proc Natl Acad Sci U S A. 2016

Daskalov et al., PLoS Biol. 2015

Habenstein et al., Angew Chem Int Ed Engl. 2015

Habenstein, Loquet. Biophys Chem. 2016

Loquet et al., Nature 2012