PhD position, Blackledge group IBS Grenoble

PhD position in Blackledge group at IBS Grenoble

NMR studies of intrinsically disordered proteins and their role in stress response

 

In spite of the ubiquitous nature of intrinsically disordered proteins (IDPs), the molecular mechanisms regulating their function remain poorly understood. In our group we use NMR spectroscopy to describe the conformational dynamics of IDPs as a function of their environment and to map their molecular interaction trajectories.

The successful candidate will investigate the physical role of IDPs that confer remarkable protective properties to diverse organisms in response to external stress such as temperature, dessication, extreme pressure or radiation. The project lies at the interface of biology, chemistry and physics and will combine NMR spectroscopy with fluorescence, small angle scattering and MD simulation. We are looking for candidates interested in applying physico-chemical approaches to resolve fascinating biological problems.

Interested candidates should send a cv, motivation letter and the names of two referees to martin.blackledge@ibs.fr or just contact me for more details.

Grenoble: Capital of the French Alps, Grenoble is an international scientific centre, with a strong international flavor. It is a pleasant city, situated at the foot of three mountain ranges offering many possibilities for cultural, outdoor and sporting activities throughout the year. Grenoble is close to the French riviera, Italy and Switzerland and is served by international and national airports. http://www.ibs.fr/jobs/about-grenoble/

Facilities: The IBS is situated on the European Photon and Neutron (EPN) campus together with its European partners, the EMBL (European Molecular Biology Laboratory), the ESRF (European Synchrotron Radiation Facility), and the ILL (Institute Laue-Langevin). This unique site provides access to state of the art equipment to analyze biological systems at different scales of resolution.

The IBS provides an international working environment with state-of-the-art NMR facilities, including 950, 850, 700 and 600MHz NMR spectrometers with both liquid-state cryoprobes and state-of-the-art solid state technology, and wet-lab facilities for cloning, expression and purification of proteins. Access is facilitated to a number of state-of-the-art biophysical platforms through the Integrated Structural Biology Grenoble (ISBG) (http://www.isbg.fr/spip.php?lang=en).

Blackledge research group: http://www.ibs.fr/groups/protein-dynamics-and-flexibility/?lang=en

Relevant recent publications:

An ultraweak interaction in the intrinsically disordered replication machinery is essential for measles virus function. Milles, Jensen, Lazert, Guseva, Ivashchenko, Communie, Maurin, Gerlier, Ruigrok, Blackledge Science Advances, 4, eaat7778 (2018)

Analytical Description of NMR Relaxation Highlights Correlated Dynamics in Intrinsically Disordered Proteins. Salvi, Abyzov, Blackledge. Angew Chem Int Ed Engl. 5614020-14024 (2017)

Identification of Dynamic Modes in an Intrinsically Disordered Protein Using Temperature-Dependent NMR Relaxation. Abyzov, Salvi, Schneider, Maurin, Ruigrok, Jensen, Blackledge J Am Chem Soc 138, 6240 (2016)

Visualizing the molecular recognition trajectory of an intrinsically disordered protein using multinuclear relaxation dispersion NMR. Schneider, Maurin, Communie, Kragelj, Hansen, Ruigrok, Jensen, Blackledge J Am Chem Soc 137, 1220 (2015)

Plasticity of an Ultrafast Interaction between Nucleoporins and Nuclear Transport Receptors. Milles, Mercadante, Aramburu, Jensen, Banterle, Koehler, Tyagi, Clarke, Shammas, Blackledge*, Gräter*, Lemke* Cell. 163, 734 (2015)

Direct observation of hierarchical protein dynamics. Lewandowski, Halse, Blackledge*, Emsley* Science 348, 578 (2015)

Large Scale Conformational Dynamics Control H5N1 Influenza Polymerase PB2 Binding to Importin α. Delaforge, Milles, Bouvignies, Bouvier, Boivin, Salvi, Maurin, Martel, Round, Lemke, Jensen, Hart, Blackledge J Am Chem Soc 137,15122 (2015)

-- 
Martin Blackledge

 
Group Leader
Research Director CEA
Protein Dynamics and Flexibility by NMR
Director Adjoint/Deputy Director
Institut de Biologie Structurale
CAMPUS EPN
71 avenue des Martyrs
CS10090
38044 Grenoble Cedex 9; 
France
martin.blackledge@ibs.fr
http://www.ibs.fr/groups/protein-dynamics-and-flexibility/?lang=en 
Telephone: +33 457 428 554