PhD position in protein NMR

The Leiden Institute of Chemistry is part of the Faculty of Mathematics and Natural Sciences of Leiden University. Leiden University is a member of the League of European Research Universities.

An opening is available in the Chromatin Organization & Dynamics group of Prof. Remus Dame for a:

PhD student on the project “Conformational changes of a DNA binding environmental sensor” (M/F), (38 hours weekly)

Project description

The chromosomes of bacteria and archaea are organized and compacted into a nucleoid. This is achieved by the action of nucleoid-associated proteins that fold the genome by bending, wrapping or bridging it. In our group we investigate using a quantitative approach the mechanisms employed by the various proteins that are involved in DNA organization and compaction both from a molecular and a cellular perspective (Dame et al., PLOS Genetics, 2011; Peeters et al., Nat Rev Microbiol, 2015; Dame & Tark-Dame, Curr Opin Cell Biol, 2016).

Currently we have an open position for a *PhD student*. The proposed project is part of a recently funded multidisciplinary research programme. The goal of this sub-project is to define by advanced NMR spectroscopy how a DNA binding protein involved in bacterial chromatin organization translates physico-chemical signals and interaction with partner proteins, via changes in structure into altered DNA binding properties.

Requirements

We are looking for outstanding experimentalists with a MSc degree in (bio)chemistry, molecular biology, (bio)physics or an equivalent degree who are motivated to collaborate in a multidisciplinary environment. In particular candidates with experience in structural biology, biomolecular NMR, biochemistry, molecular biology and protein purification are invited to apply.

Location

The project will be carried out in the Chromatin Organization & Dynamics group within the section Macromolecular Biochemistry at the Leiden Institute of Chemistry. It is in close collaboration with the NMR groups of Prof. Marcellus Ubbink and Dr. Hugo van Ingen within the same research section. The COD-group is embedded within the Cell Observatory, where investigators from the departments of biology, chemistry and physics have recently come together to facilitate multi-disciplinary research, and the Centre for Microbial Cell Biology.

Conditions of employment

The contract is for four years. The monthly salary is in accordance with the Collective Labour Agreement for Dutch Universities (CAO Nederlandse Universiteiten).

Conditions of employment

We invite applications, by sending a letter of motivation, curriculum vitae and at least two professional references to Prof. Remus Dame atjobs@bacterialchromatin.nl. Applicants selected for interview will be contacted within a month after submission of their application.

For more information on our research interests and research context check our web sites:

Chromatin Organization and Dynamics group: www.bacterialchromatin.nl

Macromolecular Biochemistry: www.universiteitleiden.nl/en/science/chemistry/macbio/

Centre for Microbial Cell Biology: www.cmcb-leiden.nl

Cell Observatory: www.universiteitleiden.nl/en/research/research-facilities/science/cell-observatory