Post-doc/PhD positions: Solid-state NMR studies of interactions, dynamics and function in molecular chaperones

The biomolecular NMR team at the Structural Biology Institute in Grenoble, France, (team leader Paul Schanda) has openings for post-doctoral/PhD researchers to work on elucidating interactions and dynamics in chaperone complexes, in research project funded by the European Research Council (ERC project ProtDyn2Function).

We are seeking candidates for a project that aims at characterizing molecular interactions and dynamics in complexes of large chaperones with unfolded/misfolded protein targets. In this project we use the solid- as well as solution-state NMR to probe molecular interactions between a hundreds-of-kilodalton large chaperone complex and “client” proteins, as well as structural and dynamical features of these complexes. The protein targets are already well established in our hands, and existing NMR characterizations/assignments as well as high-resolution EM data allow rapidly focusing on the biological/biophysical questions.

We are seeking highly motivated and creative candidates with a strong ability to work effectively and congenially with other group members.
For post-doctoral candidates, experience in biomolecular NMR spectroscopy, as well as protein production and purification are required. Strong background in chaperones would be an advantage, but not required. Experience in one of the following research areas are a strong plus: programming and data analysis (e.g. python programming), biochemical characterization of proteins and functional assays, spectroscopic techniques.
Applications for a PhD position are also welcome; experience in NMR spectroscopy is not mandatory for the PhD position, and the successful candidate should be highly motivated to learn state-of-the-art biomolecular solid-state NMR. Experience in one of the following research areas are a strong plus: programming and data analysis (e.g. python programming), biochemical characterization of proteins and functional assays, spectroscopic techniques.

The successful candidate will be part of a dynamic recently established research team, hosted in the Structural Biology Institute (IBS), in one of Europe’s most vibrant structural biology communities (centered around the European Synchrotron Facility, the ILL neutron source, EMBL Grenoble). Our group has access to brand-new NMR equipment for both solution- and solid-state NMR (2x600 MHz, 700, 850, 950 MHz with cryoprobes and fast-spinning MAS probes), a state-of-the-art electron microscopy facility, as well as a large panel of complementary biophysical techniques. The candidate will benefit from extensive expertise on advanced isotope labeling, solution- and solid-state NMR and biochemistry in the NMR group. Grenoble is a pleasant mid-sized city located at the heart of the French alps, with (public) transport connections to Paris (3 h), the mediterranean and the nearby mountain resorts.

Applications, containing a detailed CV, a list of publications and a clear statement of motivation, as well as names of 2-3 reference persons should be sent via e-mail to paul.schanda@ibs.fr.

Selected recent publications:
Observing the overall rocking motion of a protein in a crystal.
Ma P, Xue Y, Coquelle N, Haller JD, Yuwen T, Ayala I, Mikhailovskii O, Willbold D, Colletier JP, Skrynnikov NR, Schanda P.
Nature Communications. 2015; 6:8361

Probing transient conformational states of proteins by solid-state R(1ρ) relaxation-dispersion NMR spectroscopy.
Ma P, Haller JD, Zajakala J, Macek P, Sivertsen AC, Willbold D, Boisbouvier J, Schanda P.
Angewandte Chemie Int Ed Engl. 2014; 53(17):4312-7.

Atomic model of a cell-wall cross-linking enzyme in complex with an intact bacterial peptidoglycan.
Schanda P, Triboulet S, Laguri C, Bougault CM, Ayala I, Callon M, Arthur M, Simorre JP.
Journal of the American Chemical Society. 2014, 136(51):17852-60.