Postdoctoral position available in the group of Beat Vögeli at the University of Colorado at Denver

We will study protein conformation and communication networks by NMR. The focus of this project is on motor adaptors in complex dynamic protein assemblies associated with the motor cytoplasmic dynein. The Vogeli lab is part of the Department of Biochemistry and Molecular Genetics located at the Anschutz Medial Campus in Aurora/Denver. Denver is an American metropolis dating to the Old West era and a jumping-off point for ski resorts in the nearby Rocky Mountains.

 

Cytoplasmic dynein 1 (‘dynein’), a multi-protein complex of 1.2 megadaltons, is the predominant microtubule minus-end-directed motor in animals and humans. Dynein participates in a wide range of cellular activities, ranging from the transport of proteins, RNA, and vesicles to nuclear migration and cell division. Specific adapter proteins link dynein to cargo and activate the motor by forming a ternary complex with dynein and its essential co-activator dynactin. Mutations in dynein, as well as in its co-factors and adapters, have been linked to multiple neurodegenerative diseases. Several adapters have been shown to interact with the C-terminal tail of dynein light intermediate chain (LIC), which is predicted to be disordered, and mutations in the interacting segments have been implicated in spinal muscular atrophy.

It is our aim to use NMR-based experiments with the LIC C-terminal tail to provide molecular insight into how the elongated, flexible scaffold engages with functionally diverse dynein adapters. This project is realized in collaboration with the group of Dr. R. Gassmann at the Institute for Molecular and Cell Biology (IBMC) in Porto, an expert in cell division mechanism.

 

NMR facilities include Varian 500 MHz, 600 MHz and 900 MHz magnets with 5 mm triple resonance solution probes at the CU Anschutz Medical Campus, and a Varian 800 MHz magnet with a 5 mm triple resonance probe at the CU Boulder Campus. Extensive facilities for protein production and purification are available and shared resource facilities operated by the Program in Structural Biology and Biochemistry include mass spectrometry, cryo-electron microscopy, and X-ray crystallography.

 

A Ph.D. degree and excellent communication skills using the English language are required. Applicants should have a strong background in biomolecular NMR spectroscopy and biochemistry with an emphasis on standard cloning and recombinant DNA methodologies, chromatography, and protein expression and purification.

 

This position reports directly to Beat Vögeli, Ph.D. Assistant Professor in the Department of Biochemistry and Molecular Genetics.

 

Salary is commensurate with skills and experience. The University of Colorado offers a full benefits package.

 

For information on applying or any specific question related to the position, contact beat.vogeli@ucdenver.edu.