Postdoctoral position in bimolecular NMR in the Laboratory of Structural Chemistry and Biology at Gif sur Yvette, France

A Post-doc position is available for 12 months in NMR, at the Laboratory of Structural Chemistry and Biology (http://www.icsn.cnrs-gif.fr/spip.php?article1180) of the Institute of Chemistry of Natural Compounds (ICSN) in Gif-sur-Yvette, France.

The project aims at understanding the mechanisms of action of PiggyMac (Pgm), a domesticated piggyBac transposase (PB) that is involved in the ciliate Paramecium programmed genome rearrangements. Pgm (1065 amino acids) is constituted of three distinct domains, i) the catalytic domain essential for DNA excision, ii) Pgm like PB and all the PB-like transposases carry a short cysteine-rich (CR) region, iii) the three hundred last residues of Pgm, are predicted to adopt a coiled-coil structure which is a specific innovation of ciliates. The objectives of the project are to over-express (15N/13C–labelled) in E.coli and purify Pgm domains essential for its activities in order to perform structural analyses of the fragments alone and in interaction with their substrates.

The candidate will be involved in a research collaborative project, supported by the French Agence Nationale de la recherche (ANR). This project will be done in collaboration with the group of Mireille Bétermier (Programmed genome rearrangements) (http://www.i2bc.paris-saclay.fr/spip.php?rubrique81&lang=en).

See for our example recent publications in the field:

1- Morellet N, Li X, Wieninger SA, Taylor JL, Bischerour J, Moriau S, Lescop E, Bardiaux B, Mathy N, Assrir N, Bétermier M, Nilges M, Hickman AB, Dyda F, Craig NL, Guittet E. Sequence-specific DNA binding activity of the cross-brace zinc finger motif of the piggyBac transposase. Nucleic Acids Res. 2018 Jan 27. doi: 10.1093/nar/gky044.

2- Dubois E, Mathy N, Régnier V, Bischerour J, Baudry C, Trouslard R, Bétermier M. Multimerization properties of PiggyMac, a domesticated piggyBac transposase involved in programmed genome rearrangements. Nucleic Acids Res. 2017 Apr 7;45(6):3204-3216. doi: 10.1093/nar/gkw1359.

The candidate should have a strong interest in structural biology, with a strong expertise in liquid-state NMR spectroscopy, good skills in protein biochemistry and molecular biology and expertise in protein structure determination.

Our group is located at the Institute of Chemistry of Natural Compounds (ICSN) in Gif-sur-Yvette (45mn by train from the center of Paris) in a research campus mainly devoted to chemistry and biology (http://www.dr4.cnrs.fr/spip.php?article126). The campus is part of the “Campus Paris Saclay” (https://www.universite-paris-saclay.fr/en), that gathers more than 10000 researchers from all research area, among which a strong research community in Structural Biology centered in the CNRS (French National Scientific Research Center) campus in Gif-sur-Yvette, Paris 11 university in Orsay, the CEA centre of Saclay in Gif and the French national synchrotron facility SOLEIL in Gif.

Our laboratory is particularly well equipped with 4 NMR high field spectrometers ranging from 600 to 950MHz equipped with state-of-the-art cryoprobes for solution state protein NMR, all the equipment for protein production and purification and complementary biophysical techniques.

To apply, candidates should send their application by e-mail to nelly.morellet@cnrs.fr.

Applications must include a CV, a covering letter, and the names and contact details of at least two references.

 

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Nelly Morellet
Laboratoire de Chimie et Biologie Structurales
Institut de Chimie des Substances Naturelles - CNRS
1, avenue de la Terrasse
91190 Gif-sur-Yvette
France
Tél: 33-1-69-82-37-62
Fax: 33-1-69-82-37-84