Two postdoctoral positions with John Markley

Two postdoctoral positions are available immediately in the group of Professor John Markley in the Biochemistry Department, University of Wisconsin-Madison, to work on structure-function investigations concerning the biosynthesis of mitochondrial iron-sulfur proteins.
The studies involve a combination of NMR spectroscopy, small angle X-ray scattering, X-ray crystallography, calorimetry, and other biochemical/biophysical approaches. We collaborate with staff scientists at the National Magnetic Resonance facility at Madison (NMRFAM) and the Collaborative Crystallography Core (CCC).
Preference will be given to candidates with experience in biomolecular NMR spectroscopy and handling of protein samples. Experience in the use of an anaerobic chamber to handle air-sensitive proteins would be a plus.
Recent publications
Cai K, Frederick RO, Dashti H, Markley JL. Architectural Features of Human Mitochondrial Cysteine Desulfurase Complexes from Crosslinking Mass Spectrometry and Small-Angle X-Ray Scattering. Structure. 2018;26(8):1127-1136. doi: 10.1016/j.str.2018.05.017.
Cai K, Frederick RO, Tonelli M, Markley JL. Interactions of iron-bound frataxin with ISCU and ferredoxin on the cysteine desulfurase complex leading to Fe-S cluster assembly. J Inorg Biochem. 2018;183:107-116. doi: 10.1016/j.jinorgbio.2018.03.007.
Cai K, Frederick RO, Tonelli M, Markley JL. ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin. Biochemistry. 2018;57(9):1491-500. doi: 10.1021/acs.biochem.7b01234.
Tsvetkov P, Detappe A, Cai K, Keys HR, Brune Z, Ying W, Thiru P, Reidy M, Kugener G, Rossen J, Kocak M, Kory N, Tsherniak A, Santagata S, Whitesell L, Ghobrial IM, Markley JL, Lindquist S, Golub TR. Mitochondrial metabolism promotes adaptation to proteotoxic stress. Nat Chem Biol. 2019;15(7):681-689. doi: 10.1038/s41589-019-0291-9.
Interested candidates should contact John Markley ( and arrange for two letters of reference to be sent directly to me:


-- Milo
National Magnetic Resonance Facility at Madison
      An NIH-Supported Resource Center

W. Milo Westler, Ph.D.

NMRFAM Director

Distinguished Scientist
Adjunct Professor
Department of Biochemistry
University of Wisconsin-Madison
DeLuca Biochemistry Laboratories
433 Babcock Drive
Rm B160D
Madison, WI USA 53706-1544
PHONE: (608)-263-9599
FAX: (608)-263-1722