Crystallisation and Structure Determination Publications

  1. Ben-Nissan G., Belov M. E., Morgenstern D., Levin Y., Dym O., Arkind G., Lipson G., Makarov A. A. & Sharon M. (2017). Triple-Stage Mass Spectrometry Unravels the Heterogeneity of an Endogenous Protein Complex.  Analytical Chemistry. 2017 Apr , 89 (8):4708-4715.
  2. Smock R. G., Yadid I., Dym O., Clarke J. & Tawfik D. (2016). De Novo Evolutionary Emergence of a Symmetrical Protein Is Shaped by Folding Constraints.  Cell. 2016 Jan , 164 (3):476-486.
  3. Bednarczyk D., Dym O., Prabahar V., Peleg Y., Pike D. H. & Noy D. (2016). Fine Tuning of Chlorophyll Spectra by Protein-Induced Ring Deformation.  ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 2016 Jun , 55 (24):6901-6905.
  4. Goldenzweig A., Goldsmith M., Hill S. E., Gertman O., Laurino P., Ashani Y., Dym O., Unger T., Albeck S., Prilusky J., Lieberman R. L., Aharoni A., Silman I., Sussman J., Tawfik D. & Fleishman S. J. (2016). Automated Structure- and Sequence-Based Design of Proteins for High Bacterial Expression and Stability.  Molecular Cell. 2016 Jul , 63 (2):337-346.
  5. Dym O., Song W., Felder C., Roth E., Shnyrov V., Ashani Y., Xu Y., Joosten R. P., Weiner L., Sussman J. & Silman I. (2016). The impact of crystallization conditions on structure-based drug design: A case study on the methylene blue/acetylcholinesterase complex.  Protein Science. 2016 Jun , 25 (6):1096-1114.
  6. Zimhony O., Schwarz A., Raitses Gurevich G. M., Peleg Y., Dym O., Albeck S., Burstein Y. & Shakked Z. (2015). AcpM, the Meromycolate Extension Acyl Carrier Protein of Mycobacterium tuberculosis, Is Activated by the 4 '-Phosphopantetheinyl Transferase PptT, a Potential Target of the Multistep Mycolic Acid Biosynthesis.  Biochemistry. 2015 Apr , 54 (14):2360-2371.
  7. Udi Y., Grossman M., Solomonov I., Dym O., Rozenberg H., Moreno V., Cuniasse P., Dive V., Garcia Arroyo A. A. & Sagi I. (2015). Inhibition Mechanism of Membrane Metalloprotease by an Exosite-Swiveling Conformational Antibody.  Structure. 2015 Jan , 23 (1):104-115.
  8. Sobolev V., Edelman M., Dym O., Unger T., Albeck S., Kirma M. & Galili G. (2013). Structure of ALD1, a plant-specific homologue of the universal diaminopimelate aminotransferase enzyme of lysine biosynthesis.  Acta Crystallographica Section F-Structural Biology And Crystallization Communications. 2013 Feb , 69 :84-89.
  9. Xu Y., Li M., Greenblatt H., Chen W., Paz A., Dym O., Peleg Y., Chen T., Shen X., He J., Jiang H., Silman I. & Sussman J. (2012). Flexibility of the flap in the active site of BACE1 as revealed by crystal structures and molecular dynamics simulations.  Acta Crystallographica Section D-Biological Crystallography. 2012 Jan , 68 :13-25.
  10. Khersonsky O., Kiss G., Roethlisberger D., Dym O., Albeck S., Houk K. N., Baker D. & Tawfik D. (2012). Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.  Proceedings of the National Academy of Sciences of the United States of America. 2012 Jun , 109 (26):10358-10363.
  11. Sela-Passwell N. A., Kikkeri R., Dym O., Rozenberg H., Margalit R., Arad-Yellin R., Eisenstein M., Brenner O., Shoham T., Danon T., Shanzer A. & Sagi I. (2012). Antibodies targeting the catalytic zinc complex of activated matrix metalloproteinases show therapeutic potential.  Nature Medicine. 2012 Jan , 18 (1):143-147.
  12. Cohen S., Albeck S., Ben-Dov E., Cahan R., Firer M., Zaritsky A. & Dym O. (2011). Cyt1Aa Toxin: Crystal Structure Reveals Implications for Its Membrane-Perforating Function.  Journal of Molecular Biology. 2011 Nov , 413 (4):804-814.
  13. Karanicolas J., Com J. E., Chen I., Joachimiak L. A., Dym O., Peck S. H., Albeck S., Unger T., Hu W., Liu G., Delbecq S., Montelione G. T., Spiegel C. P., Liu D. R. & Baker D. (2011). A De Novo Protein Binding Pair By Computational Design and Directed Evolution.  Molecular Cell. 2011 Apr , 42 (2):250-260.
  14. Li M., Greenblatt H. M., Dym O., Albeck S., Pais A., Gunanathan C., Milstein D., Degani H. & Sussman J. (2011). Structure of Estradiol Metal Chelate and Estrogen Receptor Complex: The Basis for Designing a New Class of Selective Estrogen Receptor Modulators.  Journal of Medicinal Chemistry. 2011 May , 54 (10):3575-3580.
  15. Khersonsky O., Roethlisberger D., Wollacott A. M., Murphy P., Dym O., Albeck S., Kiss G., Houk K. N., Baker D. & Tawfik D. (2011). Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution.  Journal of Molecular Biology. 2011 Apr , 407 (3):391-412.
  16. Grossman M., Tworowski D., Dym O., Lee M., Levy Y., Murphy G. & Sagi I. (2010). The Intrinsic Protein Flexibility of Endogenous Protease Inhibitor TIMP-1 Controls Its Binding Interface and Affects Its Function.  Biochemistry. 2010 Jul , 49 (29):6184-6192.
  17. Unger T., Dym O., Albeck S., Jacobovitch Y., Bernehim R., Marom D., Pisanty O. & Breiman A. (2010). Crystal structure of the three FK506 binding protein domains of wheat FKBP73: evidence for a unique wFK73_2 domain.  Journal of Structural and Functional Genomics. 2010 Jun , 11 (2):113-23.
  18. Yadid I., Kirshenbaum N., Sharon M., Dym O. & Tawfik D. (2010). Metamorphic proteins mediate evolutionary transitions of structure.  Proceedings of the National Academy of Sciences of the United States of America. 2010 Apr , 107 (16):7287-7292.
  19. Khersonsky O., Rothlisberger D., Dym O., Albeck S., Jackson C. J., Baker D. & Tawfik D. (2010). Evolutionary Optimization of Computationally Designed Enzymes: Kemp Eliminases of the KE07 Series.  Journal of Molecular Biology. 2010 Mar , 396 (4):1025-1042.
  20. Goihberg E., Peretz M., Tel-Or S., Dym O., Shimon L., Frolow F. & Burstein Y. (2010). Biochemical and Structural Properties of Chimeras Constructed by Exchange of Cofactor-Binding Domains in Alcohol Dehydrogenases from Thermophilic and Mesophilic Microorganisms.  Biochemistry. 2010 Mar , 49 (9):1943-1953.