Voltage imaging of fast neural computations

Voltage imaging is an emerging technology that enables optical recording of membrane potentials and spiking activities of neurons at millisecond timescale. Light-sheet microscopy in behaving larval zebrafish holds the potential for expanding this technology to a brain-wide scale and elevating the level of understanding of neural computations during animal behaviors.

Based on the emergence of a bright, photostable voltage indicator in 2019,  we have been developing optical, genetic and computational methods for advancing this technology in collaboration with other laboratories.

Related literature

1. Bohm, Wang & Kawashima.
   "Voltage imaging in zebrafish using high-speed light-sheet microscopy"
   Neurophotonics. (2026)
2. Kawashima et al.
   "Voltage imaging reveals circuit computations in the raphe underlying serotonin-mediated motor vigor learning"
   Neuron. (2025) 
3. Abdelfattah*, Kawashima* et al.
   "Bright and photostable chemigenetic indicators for extended in vivo voltage imaging"
   Science. (2019)