Angiogenesis as a marker for dominant follicle selection

Liat Fellus

The selection of the dominant follicle is said to be in correlation with the formation of a richer vasculature structure in comparison with other subordinate follicles, which exposes it to higher gonadotropins levels. Using intravital microscopy we trace follicles from early stages to ovulation in vivo, and try to assess structural and functional differences in their forming vasculature.

BSA-ROX labeling of large follicles. Live imaging of mouse ovary using stereomicroscope . Red marks the contrast agent at time o after injection, green marks the same contrast agent 30 minutes after injection. At time 0 BSA-ROX marks the vasculature, but as a function of time it accumulates in
the large follicle`s antrum.