Basic Services|Fish

Background

The zebrafish (Danio rerio) is a tropical freshwater fish Native to the Himalayan region. It is currently the most used aquatic animal as a model organism.
As a model biological system, the zebrafish possesses numerous advantages for scientists. Its genome has been fully sequenced, and it has well-understood, easily observable and testable developmental behaviours. Its embryonic development is very rapid, and its embryos are relatively large, robust, and transparent, and able to develop outside their mother. Furthermore, well-characterized mutant strains are readily available.
Other advantages include the species' nearly constant size during early development, which enables simple staining techniques to be used, and the fact that its two-celled embryo can be fused into a single cell to create a homozygous embryo. The zebrafish is also demonstrably similar to mammalian models and humans in toxicity testing.

The Zebrafish unit

The zebrafish unit is located at Candiotti building.
Fish are being housed in recirculating systems in which water recirculates through mechanical, biological, and chemical filters and UV lights. 10% of the water is removed daily. The unit includes three animal rooms that contain 36 racks of 50 tanks each, a quarantine room with two additional racks and lab rooms for the scientists.
Reverse Osmosis water is being supplemented automatically by dosing pumps with salts and sodium bicarbonate to reach conductivity of 500µS and pH of 7.5. The water temperature is being kept at 28°C.

The team: Gabriella Almog, Roy Hofi, Anna Tatarin, Alla Glozman

Attending veterinarian: Noa Stettner

Publications

  • Akiva A, Malkinson G, Masic A, Kerschnitzki M, Bennet M, Fratzl P, Addadi L, Weiner S, Yaniv K.
    On the pathway of mineral deposition in larval zebrafish caudal fin bone.
    Bone. 2015 Jun;75:192-200. PMID: 25725266
  • Biran J, Tahor M, Wircer E, Levkowitz G.
    Role of developmental factors in hypothalamic function. Review.
    Front Neuroanat. 2015 Apr 21;9:47. PMID: 25954163
  • Avraham-Davidi I, Grunspan M, Yaniv K.
    Lipid signaling in the endothelium. Review.
    Exp Cell Res. 2013 May 15;319(9):1298-305. PMID: 23328305
  • Blechman J, Levkowitz G.
    Alternative Splicing of the Pituitary Adenylate Cyclase-Activating Polypeptide Receptor PAC1: Mechanisms of Fine Tuning of Brain Activity.
    Front Endocrinol (Lausanne). 2013 May 21;4:55. PMID: 23734144
  • Avraham-Davidi I, Ely Y, Pham VN, Castranova D, Grunspan M, Malkinson G, Gibbs-Bar L, Mayseless O, Allmog G, Lo B, Warren CM, Chen TT, Ungos J, Kidd K, Shaw K, Rogachev I, Wan W, Murphy PM, Farber SA, Carmel L, Shelness GS, Iruela-Arispe ML, Weinstein BM, Yaniv K.
    ApoB-containing lipoproteins regulate angiogenesis by modulating expression of VEGF receptor 1.
    Nat Med. 2012 Jun;18(6):967-73. PMID: 22581286
  • Amir-Zilberstein L1, Blechman J, Sztainberg Y, Norton WH, Reuveny A, Borodovsky N, Tahor M, Bonkowsky JL, Bally-Cuif L, Chen A, Levkowitz G.
    Homeodomain protein otp and activity-dependent splicing modulate neuronal adaptation to stress.
    Neuron. 2012 Jan 26;73(2):279-91. PMID: 22284183
  • Ben Shoham A, Malkinson G, Krief S, Shwartz Y, Ely Y, Ferrara N, Yaniv K, Zelzer E.
    S1P1 inhibits sprouting angiogenesis during vascular development.
    Development. 2012 Oct;139(20):3859-69. PMID: 22951644