CryoSTEM tomography of vitrified cells

CryoSTEM tomography (CSTET) is an alternative to the better-know cryoTEM tomography, for which thickness limitations are several hundred nanometers. 

We can probe 3D structures of cells up to around 1 micron in thickness.

This cryoSTEM tomogram shows T4 phage viruses attacking an E. coli bacterium.


The movie below shows a series of tilted cryoSTEM images of a human fibroblast cell



The movie below is stepping through the 3D volume that is reconstructed from the tilt series above. The scalebar is 400 nm.