76100 Rehovot, Israel
telephone: +972 (8) 934-2003
Members of the group:
Eyal Shimoni, Ph.D. email@example.com Yossi Englander firstname.lastname@example.org Daphna Frenkiel email@example.com Rivka Goobes firstname.lastname@example.org Rinat Sak email@example.com Nathan Zauberman firstname.lastname@example.org [Homepage] Smadar Zeidman email@example.com
DNA Organization and Survival Under StressA. Chromatin in starved cells
B. Chromatin organization in human sperm cells and fertility
- High-resolution cryo-electron microscopy and electron tomography are used to investigate the structural changes that occur within cells following exposure to harsh environmental conditions and starvation. The aims of these studies are to understand the morphological factors that allow survival under stress.
- Using electron crystallography and single-particle reconstruction techniques, we are investigating the structures of proteins that are involved in DNA protection against drugs and oxidative agents, and hence enhance survival.
DNA organization within sperm cells ('normal' and those obtained from patients treated with chemotherapy) and its effects upon fertility are being investigated.
Click here for list of all publications since 2000 (including DOIs)
A. Minsky, Structural aspects of DNA repair: the role of restricted diffusion (MicroReview), Molecular Microbiology, in press [pre-assigned DOI] D. Simberg, D. Danino, Y. Talmon, A. Minsky, ME. Ferrari, CJ. Wheeler, Barenholz Y. Phase behavior, DNA ordering and size instability of cationic lipoplexes: Relevance to optimal transfection activity. J LIPOSOME RES 13 (1): 86-87 2003
D. Frenkiel-Krispin, J. Englander, E. Shimoni, M. Eisenstein, E. Bullitt, A. Minsky, Wolf SG. How DNA is packed in bacterial spores: 3D cryo-electron microscopy of the SspC-DNA helical complex. MOL BIOL CELL 13: 2093 NOV 2002
R. Goobes, N. Kahana, O. Cohen, Minsky A. Metabolic buffering exerted by macromolecular crowding on DNA-DNA interactions: Origin and physiological significance. BIOCHEMISTRY-US 42 (8): 2431-2440 MAR 4 2003 [Read online]
S. Levin-Zaidman, J. Englander, E. Shimoni, AK. Sharma, KW. Minton, Minsky A. Ringlike structure of the Deinococcus radiodurans genome: A key to radioresistance?. SCIENCE 299 (5604): 254-256 JAN 10 2003 [Read online]
A. A. Herskovits, E. Shimoni, A. Minsky, Bibi E Accumulation of endoplasmic membranes and novel membrane-bound ribosome-signal recognition particle receptor complexes in Escherichia coli. J CELL BIOL 159 (3): 403-410 NOV 11 2002 [Read online]
D. Frenkiel-Krispin, Minsky A. Biocrystallization: a last-resort survival strategy in bacteria ASM NEWS 68 (6): 277-283 JUN 2002
R. Goobes, O. Cohen, Minsky A. Unique condensation patterns of triplex DNA: physical aspects and physiological implications. NUCLEIC ACIDS RES 30 (10): 2154-2161 MAY 15 2002 [Read online]
A. Minsky, E. Shimoni, Frenkiel-Krispin D. Stress, order and survival. NAT REV MOL CELL BIO 3 (1): 50-60 JAN 2002 [Read online]
R. Goobes, Minsky A. Thermodynamic aspects of triplex DNA formation in crowded environments. J AM CHEM SOC 123 (50): 12692-12693 DEC 19 2001 [Read online]
D. Simberg, D. Danino, Y. Talmon, et al. Phase behavior, DNA ordering, and size instability of cationic lipoplexes - Relevance to optimal transfection activity J BIOL CHEM 276 (50): 47453-47459 DEC 14 2001 [Read online]
R. Goobes and A. Minsky. Contextual equilibrium effects in DNA molecules. J. Biol. Chem. 276 (19), 16155-16160 (2001).
D. Frenkiel-Krispin, S. Levin-Zaidman, E. Shimoni, S. G. Wolf, E. J. Wachtel, T. Arad, S. E. Finkel, R. Kolter and A. Minsky. Regulated phase transitions of bacterial chromatin: a non-enzymatic pathway for generic DNA protection. EMBO J. 20, 1184 (2001).[read online]
S. Levin-Zaidman, D. Frenkiel-Krispin, E. Shimoni, I. Sabanay, S. G. Wolf and A. Minsky. Ordered intracellular RecA-DNA assemblies: a potential site of in-vivo RecA-mediated activities. Proc. Natl. Acad. Sci. USA 97, 6791 (2000).[read online]
S. G. Wolf, D. Frenkiel, T. Arad, S. E. Finkel, R. Kolter and A. Minsky. DNA protection by stress-induced biocrystallization. Nature 400, 83 (1999).[read online]
N. J. Zuidam, Y. Barenholz and A. Minsky. Chiral DNA packaging in DNA-cationic liposome assemblies. FEBS Lett. 457, 419 (1999).[read online]
A. Minsky. The chiral code: from DNA primary structures to quaternary assemblies. Chiralty 10, 405 (1998).[read online]
A. Minsky, R. Ghirlando and Z. Reich. Nucleosomes: a solution to a crowded intracellular environment. J. Theor. Biol. 188, 379 (1997).[read online]
S. Levin-Zaidman, Z. Reich, E. Wachtel and A. Minsky, Flow of structural information between four DNA conformational levels. Biochemistry 35, 2985 (1996). [read online]
Z. Reich, O. Schramm, V. Brumfeld and A. Minsky, Chiral discrimination in DNA-peptide interactions involving chiral DNA mesophases: a geometric analysis. J. Am. Chem. Soc. 118, 6345 (1996). [read online]
Z. Reich, E. Wachtel and A. Minsky, In vivo quantitative characterization of intermolecular interactions. (Communication). Biol. Chem. 270, 7045 (1995).
Z. Reich, E. Wachtel and A. Minsky, Liquid-crystalline mesophases of plasmid DNA in bacteria: Regulation of DNA supramolecular organization by supercoiling. Science 264, 1460 (1994).
M. Zemser, M. Friedman, J. Katzhendler, L. L. Greene, A. Minsky and S. Gorinstein, Relationship between functional properties and structure of ovalbumin. Protein Chemistry 13, 261 (1994).
Z. Reich, E. Wachtel, R. de-Roos and A. Minsky, Protein-independent spontaneous packaging of long DNA molecules into ordered phases. (Communication to the Editor). J. Am. Chem. Soc. 116, 7905 (1994).
Z. Reich, S. Levin-Zaidman, S. B. Gutman, T. Arad and A. Minsky, Supercoiling-regulated liquid crystalline packaging of topologically-constrained, nucleosome-free DNA molecules. Biochemistry 33, 14177 (1994).