Protocols, Reporter genes and Probes for molecular imaging

Multimodal molecular imaging

• Bimodal magnetic resonance and optical imaging of extracellular matrix remodelling by orthotopic ovarian tumours  Bochner F., Fellus-Alyagor L., Ketter D., Golani O., Biton I. & Neeman M. (2020) British Journal of Cancer.  

• Intravital imaging of vascular anomalies and extracellular matrix remodeling in orthotopic pancreatic tumors  Bochner F., Mohan V., Zinger A., Golani O., Schroeder A., Sagi I. & Neeman M. (2020)International Journal of Cancer. 146, 8, p. 2209-2217 

• Imaging Insulin Secretion from Mouse Pancreas by MRI Is Improved by Use of a Zinc-Responsive MRI Sensor with Lower Affinity for Zn2+ Ions  Martins A. F., Jordan V. C., Bochner F. et_al. (2018) Journal of the American Chemical Society. 140,50, p. 17456-17464

• Whole Organ Blood and Lymphatic Vessels Imaging (WOBLI)  Oren R., Fellus-Alyagor L., Addadi Y. et_al. (2018) Scientific Reports. 8, 1412. 

• Multimodal Correlative Preclinical Whole Body Imaging and Segmentation  Akselrod-Ballin A., Dafni H., Addadi Y., Biton I., Avni R., Brenner Y. & Neeman M. (2016) Scientific Reports. 6, 27940.

• A Novel Intravital Imaging Window for Longitudinal Microscopy of the Mouse Ovary Bochner F., Fellus-Alyagor L., Kalchenko V. (., Shinar S. & Neeman M. (2015) Scientific Reports. 5,12446.

Ferritin: Development and application of ferritin as a reporter gene for MRI

• Genetic manipulation of iron biomineralization enhances MR relaxivity in a ferritin-M6A chimeric complex
  Radoul M., Lewin L., Cohen B., Oren R., Popov S., Davidov G., Vandsburger M. H., Harmelin A., Bitton R., Greneche J., Neeman M. & Zarivach R. (2016) Scientific Reports. 6, 26550.
• Ovarian Carcinoma: Quantitative Biexponential MR Imaging Relaxometry Reveals the Dynamic Recruitment of Ferritin-expressing Fibroblasts to the Angiogenic Rim of Tumors
  Vandsburger M. H., Radoul M., Addadi Y., Mpofu S., Cohen B., Eilam R. & Neeman M. (2013) Radiology. 268, 3, p. 790-801
• Ferritin as a reporter gene for MRI: chronic liver over expression of h-ferritin during dietary iron supplementation and aging
  Ziv K., Meir G., Harmelin A., Shimoni E., Klein E. & Neeman M. (2010) NMR in Biomedicine. 23, 5, p. 523-531
  Accepted link
• Ferritin nanoparticles as magnetic resonance reporter gene
  Cohen B., Ziv K., Plaks V., Harmelin A. & Neeman M. (2009) Wiley Interdisciplinary Reviews-Nanomedicine And Nanobiotechnology. 1, 2, p. 181-188
• Ferritin as an endogenous MRI reporter for noninvasive imaging of gene expression in C6 glioma tumors
  Cohen B., Dafni H., Meir G., Harmelin A. & Neeman M. (2005) Neoplasia. 7, 2, p. 109-117
• Ferritin effect on the transverse relaxation of water: NMR microscopy at 9.4 T
  Gottesfeld Z. & Neeman M. (1996) Magnetic Resonance in Medicine. 35, 4, p. 514-520

Transglutaminase

• Magnetic Resonance Imaging Reveals Distinct Roles for Tissue Transglutaminase and Factor XIII in Maternal Angiogenesis During Early Mouse Pregnancy.
  Cohen G, Hadas R, Stefania R, Pagoto A, Ben-Dor S, Kohen F, Longo D, Elbaz M, Dekel N, Gershon E, Aime S, Neeman M.
• Arterioscler Thromb Vasc Biol. 2019 Aug;39(8):1602-1613. doi: 10.1161/ATVBAHA.119.312832. Epub 2019 Jun 13.
  PMID:31189431
• Novel MRI and fluorescent probes responsive to the Factor XIII transglutaminase activity
  Tei L., Mazooz G., Shellef Y., Avni R., Vandoorne K., Barge A., Kalchenko V., Dewhirst M. W., Chaabane L., Miragoli L., Longo D., Neeman M. & Aime S. (2010) Contrast Media & Molecular Imaging. 5, 4, p. 213-222
• Development of magnetic resonance imaging contrast material for in vivo mapping of tissue transglutaminase activity
  Mazooz G., Mehlman T., Lai T., Greenberg C., Dewhirst M. & Neeman M. (2005) Cancer Research. 65, 4, p. 1369-1375

Hyaluronan

• Hyaluronan Nanoparticles Selectively Target Plaque-Associated Macrophages and Improve Plaque Stability in Atherosclerosis
  Beldman T. J., Senders M. L., Alaarg A., Perez-Medina C., Tang J., Zhao Y., Fay F., Deichmoller J., Born B., Desclos E., van der Wel d. W. N. N., Hoebe R. A., Kohen F., Kartvelishvily E., Neeman M., Reiner T., Calcagno C., Fayad Z. A., de Winther W. M. P. J., Lutgens E., Mulder W. J. M. & Kluza E. (2017) ACS Nano. 11, 6, p. 5785-5799
• Peritoneal Adhesion and Angiogenesis in Ovarian Carcinoma Are Inversely Regulated by Hyaluronan: The Role of Gonadotropins
  Tzuman Y., Sapoznik S., Granot D., Nevo N. & Neeman M. (2010) Neoplasia. 12, 1, p. 51-60
• Magnetic resonance imaging visualization of hyaluronidase in ovarian carcinoma
  Shiftan L., Israely T., Cohen N., Frydman V., Dafni H., Stern R. & Neeman M. (2005) Cancer Research. 65, 22, p. 10316-10323
• Kinetic analysis of hyaluronidase activity using a bioactive MRI contrast agent
  Shiftan L. & Neeman M. (2006) Contrast Media & Molecular Imaging. 1, 3, p. 106-112
• Hyaluronic acid as an anti-angiogenic shield in the preovulatory rat follicle
  Tempel C., Gilead A. & Neeman M. (2000) Biology of Reproduction. 63, 1, p. 134-140

biotin-BSA-GdDTPA

• Labeling fibroblasts with biotin-BSA-GdDTPA-FAM for tracking of tumor-associated stroma by fluorescence and MR imaging
  Granot D., Kunz-Schughart L. & Neeman M. (2005) Magnetic Resonance in Medicine. 54, 4, p. 789-797
• Overexpression of vascular endothelial growth factor 165 drives peritumor interstitial convection and induces lymphatic drain: Magnetic resonance imaging, confocal microscopy, and histological tracking of triple-labeled albumin
  Dafni H., Israely T., Bhujwalla Z., Benjamin L. & Neeman M. (2002) Cancer Research. 62, 22, p. 6731-6739

Avidin chase

• Modulation of the pharmacokinetics of macromolecular contrast material by avidin chase: MRI, optical, and inductively coupled plasma mass spectrometry tracking of triply labeled albumin
  Dafni H., Gilead A., Nevo N., Eilam R., Harmelin A. & Neeman M. (2003) Magnetic Resonance in Medicine. 50, 5, p. 904-914